Product nameAnti-IWS1 antibody - N-terminal
See all IWS1 primary antibodies
DescriptionRabbit polyclonal to IWS1 - N-terminal
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Sheep, Rabbit, Goat, Cow, Dog, Pig, Saccharomyces cerevisiae, Chimpanzee, Drosophila melanogaster, Rhesus monkey, Gorilla, Common marmoset, Orangutan, Bat
- HeLa, 293T, Jurkat, mouse TCMK-1 and mouse NIH3T3 cell lysates
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab195309 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/1000 - 1/5000. Predicted molecular weight: 92 kDa.|
Sequence similaritiesBelongs to the IWS1 family.
Contains 1 TFIIS N-terminal domain.
- Information by UniProt
- DKFZp761G0123 antibody
- FLJ10006 antibody
- FLJ14655 antibody
Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: ab195309 used for IP at 6 µg per reaction. For blotting immunoprecipitated IWS1, ab195309 was used at 0.4 µg/mL.
All lanes : Anti-IWS1 antibody - N-terminal (ab195309) at 0.4 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : Mouse TCMK-1 cell lysate
Lane 5 : Mouse NIH3T3 cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 92 kDa
Exposure time: 30 seconds
ab195309 has not yet been referenced specifically in any publications.