Overview

  • Product name

    Anti-IZUMO1 antibody [125] - BSA and Azide free
    See all IZUMO1 primary antibodies
  • Description

    Rat monoclonal [125] to IZUMO1 - BSA and Azide free
  • Host species

    Rat
  • Tested applications

    Suitable for: IP, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Tissue, cells or virus corresponding to Mouse IZUMO1. Mouse sperm

  • Positive control

    • WT mouse sperm lysate; mouse testis and sperm lysates; human testis and sperm lysates; mouse spermatozoa.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituents: 50% PBS, 50% Glycerol

    filter sterilized
  • Concentration information loading...
  • Purity

    Ion Exchange Chromatography
  • Clonality

    Monoclonal
  • Clone number

    125
  • Isotype

    IgG2a
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab211626 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/100.
WB 1/1000.
ICC/IF 1/250.

Target

  • Function

    Involved in the fertilization process. May be involved in the fusion of the sperm with the egg.
  • Tissue specificity

    Sperm-specific (at protein level). Detectable on sperm surface only after the acrosome reaction.
  • Sequence similarities

    Belongs to the Izumo family.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
  • Post-translational
    modifications

    N-glycosylated. Glycosylation is not essential for fusion and for proper protein trafficking in sperm.
    Phosphorylated.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ61440 antibody
    • IZUM1_HUMAN antibody
    • IZUMO antibody
    • Izumo sperm egg fusion 1 antibody
    • Izumo sperm-egg fusion protein 1 antibody
    • Izumo1 antibody
    • MGC34799 antibody
    • OBF antibody
    • Oocyte binding/fusion factor antibody
    • Sperm specific protein izumo antibody
    • Sperm-specific protein izumo antibody
    see all

Images

  • All lanes : Anti-IZUMO1 antibody [125] - BSA and Azide free (ab211626) at 1/1000 dilution

    Lane 1 : WT mouse sperm lysate
    Lane 2 : IZUMO1 Knock out mouse sperm lysate

    Lysates/proteins at 10 µg per lane.
  • All lanes : Anti-IZUMO1 antibody [125] - BSA and Azide free (ab211626) at 1/1000 dilution

    Lane 1 : mouse testis lysate
    Lane 2 : mouse sperm lysate

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab97057) at 1/10000 dilution


    SDS Page 10-20% gradient gel.

  • All lanes : Anti-IZUMO1 antibody [125] - BSA and Azide free (ab211626) at 1/100 dilution

    Lane 1 : human testis lysate
    Lane 2 : human sperm lysate

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab97057) at 1/10000 dilution
  • Immunofluorescent analysis of mouse spermatozoa labeling IZUMO1 using ab211626 at 4 μg/mL. An Alexa Fluor 488 conjugated anti-rat IgG was used as secondary antibody.

References

ab211626 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab211626.
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