Overview

  • Product name

  • Description

    Rabbit polyclonal to IZUMO1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WB, Functional Studiesmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    This product was produced with the following immunogens:
    Synthetic peptide corresponding to Human IZUMO1 aa 34-47 conjugated to Keyhole Limpet Haemocyanin (KLH) (Cysteine residue).
    Sequence:

    KSLEKDYLPGHLDA


    Database link: Q8IYV9

    Synthetic peptide corresponding to Human IZUMO1 aa 337-350 conjugated to Keyhole Limpet Haemocyanin (KLH) (Cysteine residue).
    Sequence: TQVPKEKATDSRQQ
    Database link: Q8IYV9

    Synthetic peptide within Human IZUMO1 aa 227-284 conjugated to Keyhole Limpet Haemocyanin (KLH) (Cysteine residue). The exact sequence is proprietary.
    Sequence: ATTESSISLQPLQ
    Database link: Q8IYV9

  • Positive control

    • Human sperm lysate; mouse sperm lysate; human sperm; mouse testis tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.1% Sodium azide
  • Purity

    Whole antiserum
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab211623 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/300.
IHC-P 1/100. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
WB 1/1000.
Functional Studies Use at an assay dependent concentration.

Inhibition of sperm fusion with egg

Target

  • Function

    Involved in the fertilization process. May be involved in the fusion of the sperm with the egg.
  • Tissue specificity

    Sperm-specific (at protein level). Detectable on sperm surface only after the acrosome reaction.
  • Sequence similarities

    Belongs to the Izumo family.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
  • Post-translational
    modifications

    N-glycosylated. Glycosylation is not essential for fusion and for proper protein trafficking in sperm.
    Phosphorylated.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ61440 antibody
    • IZUM1_HUMAN antibody
    • IZUMO antibody
    • Izumo sperm egg fusion 1 antibody
    • Izumo sperm-egg fusion protein 1 antibody
    • Izumo1 antibody
    • MGC34799 antibody
    • OBF antibody
    • Oocyte binding/fusion factor antibody
    • Sperm specific protein izumo antibody
    • Sperm-specific protein izumo antibody
    see all

Images

  • Anti-IZUMO1 antibody (ab211623) at 1/1000 dilution + human sperm lysate at 20 µg
  • Anti-IZUMO1 antibody (ab211623) at 1/1000 dilution + mouse sperm lysate at 10 µg


    10-20% SDS Page gradient gel.

  • Immunohistochemical analysis of formalin fixed and paraffin embedded mouse testis labeling IZUMO1 using ab211623 at 1/100 dilution followed by secondary detection usin an Alexafluor 488 goat anti rabbit (H&L) IgG at 1/1000 dilution (green). Nuclear counterstain: DAPI (blue). 

  • Immunofluorescent analysis of human sperm labeling IZUMO1 using ab211623 at 1/100 dilution. An Alexa Fluor594 conjugated anti-rabbit IgG at 1/1000 dilution was used as secondary antibody.

References

ab211623 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab211623.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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