Western blot abreview for Anti-JAB1 antibody

Excellent
Abreviews
Application
Western blot
Loading amount
35 µg
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Sample
Mouse Cell lysate - whole cell (HEK293, HCT116, U2OS and Mouse Embryonic Fibroblas)
Specification
HEK293, HCT116, U2OS and Mouse Embryonic Fibroblas
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: 5% milk in TBS tween (0.2% tween)
Dilution
1/2500

Secondary antibody

Name
Non-Abcam antibody was used: Pierce Anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
ECL
Bands
Specific: 35 kDa Non-specific: 50 kDa
Exposure
10 second(s)
Positive control
Lane 1 is HEK293 lysate, lane 2 is HCT116 lysate, lane 3 is U2OS lysate and lane 4 is Mouse Embryonic Fibroblast lysate (all 35 micrograms whole cell lysate)

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Submitted Dec 19 2013

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