Overview

  • Product name

  • Description

    Rabbit polyclonal to JAB1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Cow, Pig, Xenopus laevis, Zebrafish, Xenopus tropicalis
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-198 of Human JAB1.

  • Positive control

    • Mouse brain, HeLa whole cell lysates; HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab137326 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 38 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Probable protease subunit of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of the SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. In the complex, it probably acts as the catalytic center that mediates the cleavage of Nedd8 from cullins. It however has no metalloprotease activity by itself and requires the other subunits of the CSN complex. Interacts directly with a large number of proteins that are regulated by the CSN complex, confirming a key role in the complex.
  • Sequence similarities

    Belongs to the peptidase M67A family. CSN5 subfamily.
    Contains 1 MPN (JAB/Mov34) domain.
  • Domain

    The JAMM motif is essential for the protease activity of the CSN complex resulting in deneddylation of cullins. It constitutes the catalytic center of the complex.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • 38 kDa Mov34 homolog antibody
    • COP9 (constitutive photomorphogenic) homolog subunit 5 antibody
    • COP9 constitutive photomorphogenic homolog subunit 5 antibody
    • COP9 signalosome complex subunit 5 antibody
    • COP9 signalosome subunit 5 antibody
    • Cop9 subunit 5 antibody
    • COPS 5 antibody
    • cops5 antibody
    • CSN 5 antibody
    • CSN5 antibody
    • CSN5_HUMAN antibody
    • JAB 1 antibody
    • Jun activation domain binding protein 1 antibody
    • Jun activation domain binding protein antibody
    • Jun activation domain-binding protein 1 antibody
    • MGC3149 antibody
    • MOV 34 antibody
    • MOV34 antibody
    • MOV34 family, 38-KD member antibody
    • SGN 5 antibody
    • SGN5 antibody
    • Signalosome subunit 5 antibody
    see all

Images

  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells labelling JAB1, using ab137326 (green) at a 1/100 dilution. Cell nuclei were stained with Hoechst 33342 (blue).
  • Anti-JAB1 antibody (ab137326) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size: 38 kDa



    10% SDS Page
  • Anti-JAB1 antibody (ab137326) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size: 38 kDa



    10% SDS Page

References

ab137326 has not yet been referenced specifically in any publications.

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