Overview

  • Product name
  • Description
    Rabbit polyclonal to Jagged1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide: N-KASRGNDRNRIVLPFS conjugated to KLH, corresponding to amino acids 110-125 of Human Jagged1

Properties

Applications

Our Abpromise guarantee covers the use of ab7771 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-P 1/100.
WB 1/500. Detects a band of approximately 150 kDa (predicted molecular weight: 134 kDa). 150 kDa in mouse liver lysate and a 75 kDa band in human brain and kidney lysates.

Target

  • Function
    Ligand for multiple Notch receptors and involved in the mediation of Notch signaling. May be involved in cell-fate decisions during hematopoiesis. Seems to be involved in early and late stages of mammalian cardiovascular development. Inhibits myoblast differentiation (By similarity). Enhances fibroblast growth factor-induced angiogenesis (in vitro).
  • Tissue specificity
    Widely expressed in adult and fetal tissues. In cervix epithelium expressed in undifferentiated subcolumnar reserve cells and squamous metaplasia. Expression is up-regulated in cervical squamous cell carcinoma. Expressed in bone marrow cell line HS-27a which supports the long-term maintenance of immature progenitor cells.
  • Involvement in disease
    Defects in JAG1 are the cause of Alagille syndrome type 1 (ALGS1) [MIM:118450]. Alagille syndrome is an autosomal dominant multisystem disorder defined clinically by hepatic bile duct paucity and cholestasis in association with cardiac, skeletal, and ophthalmologic manifestations. There are characteristic facial features and less frequent clinical involvement of the renal and vascular systems.
    Defects in JAG1 are a cause of tetralogy of Fallot (TOF) [MIM:187500]. TOF is a congenital heart anomaly which consists of pulmonary stenosis, ventricular septal defect, dextroposition of the aorta (aorta is on the right side instead of the left) and hypertrophy of the right ventricle. This condition results in a blue baby at birth due to inadequate oxygenation. Surgical correction is emergent.
  • Sequence similarities
    Contains 1 DSL domain.
    Contains 15 EGF-like domains.
  • Developmental stage
    Expressed in 32-52 days embryos in the distal cardiac outflow tract and pulmonary artery, major arteries, portal vein, optic vesicle, otocyst, branchial arches, metanephros, pancreas, mesocardium, around the major bronchial branches, and in the neural tube.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AGS antibody
    • AHD antibody
    • AWS antibody
    • CD 339 antibody
    • CD339 antibody
    • CD339 antigen antibody
    • Headturner antibody
    • hJ1 antibody
    • Htu antibody
    • Jag 1 antibody
    • Jag1 antibody
    • JAG1_HUMAN antibody
    • Jagged 1 antibody
    • Jagged1 (Alagille syndrome) antibody
    • Jagged1 antibody
    • JAGL1 antibody
    • MGC104644 antibody
    • OTTHUMP00000030278 antibody
    • Protein jagged-1 antibody
    • Ser 1 antibody
    • Ser1 antibody
    • Serrate 1 antibody
    • Slalom antibody
    see all

Images

  • Formalin-fixed paraffin embedded IHC of normal human cervial tissues (40x magnification) stained with ab7771 (1/100 dilution). The slides were counterstained with hematoxylin

  • Staining of Human corneal epithelial cells with anti-Jagged1 (ab7771, 1/500). The Jagged1 (green staining) is localised to the cytoplasm and cell nucleus and is consistent with reports in the literature. The nucleus is stained with Bis benzimide (1/500, blue staining).

  • Formalin-fixed paraffin embedded IHC of human cervical cancer tissues (40x magnification) stained with ab7771 (1/100 dilution). The slides were counterstained with hematoxylin
  • ab7771 Rabbit polyclonal to Jagged1 (1/500) with secondary Goat anti-rabbit IgG antibody ab6721 (1/5000)
    Exposure time: 1 minute. Expected molecular weight: 134 kDa

    Lanes 1 to 3 and 5: 20µg of cell lysate per lane
    Lane 4: 20 µl of 1x LDS loading buffer

    Lane 1: Human Brain tissue lysate
    Lane 2: Human Kidney tissue Lysate
    Lane 3: Human liver tissue lysate
    Lane 4: 1x LDS loading buffer
    Lane 5: Mouse liver lysate

     

References

This product has been referenced in:
  • Luo X  et al. Inhibition of notch signaling pathway temporally postpones the cartilage degradation progress of temporomandibular joint arthritis in mice. J Craniomaxillofac Surg 46:1132-1138 (2018). Read more (PubMed: 29779621) »
  • Tripathi R  et al. Jagged-1 induced molecular alterations in HPV associated invasive squamous cell and adenocarcinoma of the human uterine cervix. Sci Rep 8:9359 (2018). Read more (PubMed: 29921897) »
See all 38 Publications for this product

Customer reviews and Q&As

1-10 of 16 Abreviews or Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Pig Tissue lysate - whole (Newborn kidney)
Gel Running Conditions
Reduced Denaturing
Loading amount
40 µg
Specification
Newborn kidney
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Mar 01 2018

Abcam has not validated the combination of species/application used in this Abreview.
Application
Flow Cytometry
Sample
Mouse Cell (Skin)
Permeabilization
No
Specification
Skin

Abcam user community

Verified customer

Submitted Nov 20 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Sample
Human Cell (Breast cancer cell line)
Specification
Breast cancer cell line
Permeabilization
Yes - 0.1% Triton-X in PBS
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Aug 16 2013

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH 9
Sample
Cow Tissue sections (Liver)
Specification
Liver
Permeabilization
Yes - Triton X
Fixative
Formaldehyde

Dr. Daniel Murta

Verified customer

Submitted Jun 07 2013

Answer

Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from the Anti-Jagged1 antibody (ab7771) .

I would like to reassure you that this antibody is tested and covered by our 6 month guarantee for the applications IHC-FoFr, ICC/IF, IHC-P, WB, IHC-Fr with mouse and human samples. In the event that a product is not functioning in the tested applications and species cited on the product data sheet, we will be pleased to provide a credit note, free of charge replacement or refund.

I would like to investigate this particular case further for you, and also obtain more information for our quality monitoring records. In order to proceed with this, I have enclosed a technical questionnaire below. I would appreciate if you could complete this. It will help you put the information we require together very easily. If I understood you right you have had problems in obtaining good results in WB? At the end of this email I attached the WB questionnaire. If you had problems with another application please let me know and I will send you the suitable questionnaire.

I would appreciate if you could also provide the image in another format. Regrettably I am not able to open it .

Thank you for your time and cooperation. We look forward to receiving the completed questionnaire.

Questionnaire:

Order Details
Antibody code:

Problem
Choose: Non-specific band Multiple bands No signal or weak signal High background

Lot number

Purchase order number
or preferably Abcam order number:



General Information
Antibody storage conditions (temperature/reconstitution etc)


Description of the problem (high background, wrong band size, more bands, no band etc.)


Sample (Species/Cell extract/Nuclear extract/Purified protein/Recombinant protein etc.)


Sample preparation (Buffer/Protease inhibitors/Heating sample etc.)


Amount of protein loaded


Electrophoresis/Gel conditions (Reducing or Non-reducing gel, % of the gel etc.)


Transfer and blocking conditions (Buffer/time period, Blocking agent etc.)


Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)


Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)


Detection method (ECL, ECLPlus etc.)


Positive and negative controls used (please specify)



Optimization attempts (problem solving)
How many times have you tried the Western?



Have you run a "No Primary" control?
Yes No

Do you obtain the same results every time?
Yes No
e.g. are the background bands always in the same place?


What steps have you altered?


Additional Notes:


Image:
We would appreciate if you are able to provide an image (including molecular weight markers) which would help us to asess the results.

Read More

Answer

I have heard back for the supplying lab.

The IHC data we have on the datasheet were contributed by their endusers. Unfortunately, they do not have a specific protocol available and all conditions must be user optimized.

I am sorry to not have more detailed information in that regard.

However, our in-house IHC-P protocol can be found here:

https://www.abcam.com/index.html?pageconfig=resource&rid=11384

I hope that this will be of help.

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https://www.abcam.com/abreviews

Read More

Answer

Thank you for your reply and the image.

In the image I am not quite able to tell where the nuclei or potential plasma membranes are as there is no reference point. Does the image reflect just 1 cell or a line up of cells?
Would you have an image with DAPI staining of the nuclei (or any other counter stain) together with the Jagged1 staining? That would help a lot at least to know where the cells are located.

Here is the link to the Human protein Atlas for Jagged1
http://www.proteinatlas.org/ENSG00000101384
for the subcellular localization this link shows you in green where the protein is localized: http://www.proteinatlas.org/ENSG00000101384/subcellular

I have not yet heard back from the lab and will follow up with them as well.

I look forward to hear back from you.

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https://www.abcam.com/abreviews

Read More

Answer

Thank you for contacting us.

I am checking with the lab regarding the protocol, but could you please send me in the meantime an image showing the staining you are obtaining?

I look forward to your reply and helping you further.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement of ab7771 from lot GR14057-8. This is the same parent lot as the antibody you tried, just aliquoted later (-8). We will ship this on Monday for delivery on Tuesday. To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Read More

Answer

Thank you for taking time tocontact us. I am sorry to hear that this antibody is not providing satisfactory results.

Are the samples you are staining the same as you have tried previously?

What dilutions of the antibody have you tried?

The antibody has lost specificity while it was stored. Do you see high background now, or no staining at all? Would you be able to send images of the previous stainings and new results?

In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund.

I hope this information is helpful, and I thank you for your cooperation.

Read More

1-10 of 16 Abreviews or Q&A

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