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Cardiovascular Angiogenesis Inhibitors
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Jagged1 antibody [EPR4290] - BSA and Azide free (ab184785)

  • Datasheet
  • Certificate of Compliance
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Western blot - Anti-Jagged1 antibody [EPR4290] - BSA and Azide free (ab184785)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Jagged1 antibody [EPR4290] - BSA and Azide free (ab184785)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Jagged1 antibody [EPR4290] - BSA and Azide free (ab184785)
  • Anti-Jagged1 antibody [EPR4290] - BSA and Azide free (ab184785)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4290] to Jagged1 - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Human

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Overview

  • Product name

    Anti-Jagged1 antibody [EPR4290] - BSA and Azide free
    See all Jagged1 primary antibodies
  • Description

    Rabbit monoclonal [EPR4290] to Jagged1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC/IF
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human Jagged1 aa 1150-1250 (C terminal). The exact sequence is proprietary.

  • Positive control

    • HepG2 and NIH:OVCAR-3 cell lysate. Bladder cancer tissue. HeLa cells.
  • General notes

    ab184785 is the carrier-free version of ab109536. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab184785 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.20
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR4290
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Inhibitors
    • Neuroscience
    • Neurology process
    • Notch Pathway
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Stem Cells
    • Signaling Pathways
    • Notch
    • Surface Molecules
    • Stem Cells
    • Hematopoietic Progenitors
    • Surface Molecules

Associated products

  • Alternative Versions

    • Anti-Jagged1 antibody [EPR4290] (ab109536)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant human Jagged1 protein (Fc Chimera Active) (ab108575)

Applications

Our Abpromise guarantee covers the use of ab184785 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 134 kDa.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Function

      Ligand for multiple Notch receptors and involved in the mediation of Notch signaling. May be involved in cell-fate decisions during hematopoiesis. Seems to be involved in early and late stages of mammalian cardiovascular development. Inhibits myoblast differentiation (By similarity). Enhances fibroblast growth factor-induced angiogenesis (in vitro).
    • Tissue specificity

      Widely expressed in adult and fetal tissues. In cervix epithelium expressed in undifferentiated subcolumnar reserve cells and squamous metaplasia. Expression is up-regulated in cervical squamous cell carcinoma. Expressed in bone marrow cell line HS-27a which supports the long-term maintenance of immature progenitor cells.
    • Involvement in disease

      Defects in JAG1 are the cause of Alagille syndrome type 1 (ALGS1) [MIM:118450]. Alagille syndrome is an autosomal dominant multisystem disorder defined clinically by hepatic bile duct paucity and cholestasis in association with cardiac, skeletal, and ophthalmologic manifestations. There are characteristic facial features and less frequent clinical involvement of the renal and vascular systems.
      Defects in JAG1 are a cause of tetralogy of Fallot (TOF) [MIM:187500]. TOF is a congenital heart anomaly which consists of pulmonary stenosis, ventricular septal defect, dextroposition of the aorta (aorta is on the right side instead of the left) and hypertrophy of the right ventricle. This condition results in a blue baby at birth due to inadequate oxygenation. Surgical correction is emergent.
    • Sequence similarities

      Contains 1 DSL domain.
      Contains 15 EGF-like domains.
    • Developmental stage

      Expressed in 32-52 days embryos in the distal cardiac outflow tract and pulmonary artery, major arteries, portal vein, optic vesicle, otocyst, branchial arches, metanephros, pancreas, mesocardium, around the major bronchial branches, and in the neural tube.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession P78504 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 182 Human
      • Entrez Gene: 16449 Mouse
      • Entrez Gene: 29146 Rat
      • Omim: 601920 Human
      • SwissProt: P78504 Human
      • SwissProt: Q9QXX0 Mouse
      • SwissProt: Q63722 Rat
      • Unigene: 224012 Human
      • Unigene: 22398 Mouse
      • Unigene: 88804 Rat
      see all
    • Alternative names

      • AGS antibody
      • AHD antibody
      • AWS antibody
      • CD 339 antibody
      • CD339 antibody
      • CD339 antigen antibody
      • Headturner antibody
      • hJ1 antibody
      • Htu antibody
      • Jag 1 antibody
      • Jag1 antibody
      • JAG1_HUMAN antibody
      • Jagged 1 antibody
      • Jagged1 (Alagille syndrome) antibody
      • Jagged1 antibody
      • JAGL1 antibody
      • MGC104644 antibody
      • OTTHUMP00000030278 antibody
      • Protein jagged-1 antibody
      • Ser 1 antibody
      • Ser1 antibody
      • Serrate 1 antibody
      • Slalom antibody
      see all

    Images

    • Western blot - Anti-Jagged1 antibody [EPR4290] - BSA and Azide free (ab184785)
      Western blot - Anti-Jagged1 antibody [EPR4290] - BSA and Azide free (ab184785)
      All lanes : Anti-Jagged1 antibody [EPR4290] (ab109536) at 1/10000 dilution

      Lane 1 : Wild-type HAP1 whole cell lysate
      Lane 2 : JAG1 knockout HAP1 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 134 kDa
      Observed band size: 180 kDa
      why is the actual band size different from the predicted?



      Lanes 1 - 2: Merged signal (red and green). Green - ab109536 observed at 180 kDa. Red - loading control, ab9484, observed at 37 kDa.

      ab109536 was shown to recognize in wild-type HAP1 cells as signal was lost at the expected MW in JAG1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and JAG1 knockout samples were subjected to SDS-PAGE. Ab109536 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109536).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Jagged1 antibody [EPR4290] - BSA and Azide free (ab184785)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Jagged1 antibody [EPR4290] - BSA and Azide free (ab184785)

      Immunohistochemical staining of paraffin embedded human kidney using purified ab109536 at a dilution of 1/300. A pre-diluted HRP polymer for rabbit IgG was used as the secondary and the sample was counter stained with hematoxylin. 

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109536).

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Jagged1 antibody [EPR4290] - BSA and Azide free (ab184785)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Jagged1 antibody [EPR4290] - BSA and Azide free (ab184785)

      Immunohistochemical staining of Jagged1 in paraffin-embedded bladder cancer tissue using unpurified ab109536 at a dilution of 1/250.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109536).

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Anti-Jagged1 antibody [EPR4290] - BSA and Azide free (ab184785)
      Anti-Jagged1 antibody [EPR4290] - BSA and Azide free (ab184785)

    Protocols

    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (2)

    Publishing research using ab184785? Please let us know so that we can cite the reference in this datasheet.

    ab184785 has been referenced in 2 publications.

    • Yoshida R  et al. The pathological significance of Notch1 in oral squamous cell carcinoma. Lab Invest 93:1068-81 (2013). Human . PubMed: 23938602
    • Yang W  et al. Ptpn11 deletion in a novel progenitor causes metachondromatosis by inducing hedgehog signalling. Nature 499:491-5 (2013). Mouse . PubMed: 23863940

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