Anti-JAGN1 antibody (ab237735)
Key features and details
- Rabbit polyclonal to JAGN1
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-JAGN1 antibody -
Description
Rabbit polyclonal to JAGN1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow -
Immunogen
Recombinant fragment corresponding to Human JAGN1 aa 1-100.
Database link: Q8N5M9 -
Positive control
- WB: HL-60 whole cell lysate. IHC-P: Human colon cancer and adrenal gland tissues. ICC/IF: A549 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity greater than 95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab237735 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/2000. Predicted molecular weight: 21 kDa.
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ICC/IF |
1/50 - 1/200.
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IHC-P |
1/20 - 1/200.
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Notes |
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WB
1/500 - 1/2000. Predicted molecular weight: 21 kDa. |
ICC/IF
1/50 - 1/200. |
IHC-P
1/20 - 1/200. |
Target
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Function
May be required for endoplasmic reticulum organization. -
Sequence similarities
Belongs to the jagunal family. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 539101 Cow
- Entrez Gene: 84522 Human
- Entrez Gene: 67767 Mouse
- Entrez Gene: 502872 Rat
- Omim: 616012 Human
- SwissProt: Q2NKY9 Cow
- SwissProt: Q8N5M9 Human
- SwissProt: Q5XKN4 Mouse
see all -
Alternative names
- FLJ14602 antibody
- GL009 antibody
- JAGN 1 antibody
see all
Images
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Anti-JAGN1 antibody (ab237735) at 1/500 dilution + HL-60 (human promyelocytic leukemia cell line) whole cell lysate
Secondary
Goat polyclonal to rabbit at 1/10000 dilution
Predicted band size: 21 kDa -
Paraffin-embedded human colon cancer tissue stained for JAGN1 using ab237735 at 1/100 dilution in immunohistochemical analysis.
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Paraffin-embedded human adrenal gland tissue stained for JAGN1 using ab237735 at 1/100 dilution in immunohistochemical analysis.
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A549 (human lung carcinoma cell line) cells stained for JAGN1 (green) using ab237735 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab237735 has not yet been referenced specifically in any publications.