Anti-JAK1 antibody (ab47435)
Key features and details
- Rabbit polyclonal to JAK1
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-JAK1 antibody
See all JAK1 primary antibodies -
Description
Rabbit polyclonal to JAK1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat
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Immunogen
Synthetic non - phosphopeptide (Human) from around the phosphorylation site of Tyrosine 1022
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab47435 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use a concentration of 1 µg/ml.
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| IHC-P |
Use at an assay dependent concentration.
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| WB |
1/500 - 1/1000. Detects a band of approximately 132 kDa (predicted molecular weight: 132 kDa).
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| Notes |
|---|
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ICC/IF
Use a concentration of 1 µg/ml. |
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IHC-P
Use at an assay dependent concentration. |
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WB
1/500 - 1/1000. Detects a band of approximately 132 kDa (predicted molecular weight: 132 kDa). |
Target
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Function
Tyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway. Kinase partner for the interleukin (IL)-2 receptor. -
Tissue specificity
Expressed at higher levels in primary colon tumors than in normal colon tissue. The expression level in metastatic colon tumors is comparable to the expression level in normal colon tissue. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.
Contains 1 FERM domain.
Contains 1 protein kinase domain.
Contains 1 SH2 domain. -
Domain
Possesses two phosphotransferase domains. The second one probably contains the catalytic domain (By similarity), while the presence of slight differences suggest a different role for domain 1.
The FERM domain mediates interaction with JAKMIP1. -
Cellular localization
Endomembrane system. Wholly intracellular, possibly membrane associated. - Information by UniProt
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Database links
- Entrez Gene: 3716 Human
- Entrez Gene: 84598 Rat
- Omim: 147795 Human
- SwissProt: P23458 Human
- Unigene: 207538 Human
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Alternative names
- JAK 1 antibody
- JAK 1A antibody
- JAK 1B antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-JAK1 antibody (ab47435)ICC/IF image of ab47435 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47435, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Western blot - Anti-JAK1 antibody (ab47435)All lanes : Anti-JAK1 antibody (ab47435) at 1/500 dilution
Lane 1 : MCF7 cell extract treated with EGF
Lane 2 : MCF7 cell extract treated with EGF with peptide
Predicted band size: 132 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JAK1 antibody (ab47435)Immunohistochemical staining of paraffin embedded human breast carcinoma tissue using ab47435 at dilution of 1/50-1/100. Right panel treated with peptide and left panel untreated
Protocols
Datasheets and documents
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Datasheet download
References (28)
ab47435 has been referenced in 28 publications.
- Bie C et al. Insulin-Like Growth Factor 1 Receptor Drives Hepatocellular Carcinoma Growth and Invasion by Activating Stat3-Midkine-Stat3 Loop. Dig Dis Sci N/A:N/A (2021). PubMed: 33559791
- Wang K et al. Glutathione S-transferase ? 1 promotes the proliferation, migration and invasion, and inhibits the apoptosis of non-small cell lung cancer cells, via the JAK/STAT3 signaling pathway. Mol Med Rep 23:N/A (2021). PubMed: 33236161
- Alves-Silva T et al. Interleukin-6 deficiency modulates testicular function by increasing the expression of suppressor of cytokine signaling 3 (SOCS3) in mice. Sci Rep 11:11456 (2021). PubMed: 34075113
- Zhou Y et al. MiR-769-5p inhibits cancer progression in oral squamous cell carcinoma by directly targeting JAK1/STAT3 pathway. Neoplasma 67:528-536 (2020). PubMed: 32064884
- Sun Z et al. NHE1 Mediates 5-Fu Resistance in Gastric Cancer via STAT3 Signaling Pathway. Onco Targets Ther 13:8521-8532 (2020). PubMed: 32904684
