Product nameAnti-JAK1 antibody
See all JAK1 primary antibodies
DescriptionRabbit polyclonal to JAK1
SpecificityDetects endogenous levels of JAK1 protein
Tested applicationsSuitable for: ICC/IF, IHC-P, ELISA, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
Synthetic non - phosphopeptide (Human) from around the phosphorylation site of Tyrosine 1022
- Human breast carcinoma tissue. Extracts from MCF7 cells.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg+2 and Ca+2
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAffinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen
Our Abpromise guarantee covers the use of ab47435 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|IHC-P||Use at an assay dependent concentration.|
|WB||1/500 - 1/1000. Detects a band of approximately 132 kDa (predicted molecular weight: 132 kDa).|
FunctionTyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway. Kinase partner for the interleukin (IL)-2 receptor.
Tissue specificityExpressed at higher levels in primary colon tumors than in normal colon tissue. The expression level in metastatic colon tumors is comparable to the expression level in normal colon tissue.
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.
Contains 1 FERM domain.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
DomainPossesses two phosphotransferase domains. The second one probably contains the catalytic domain (By similarity), while the presence of slight differences suggest a different role for domain 1.
The FERM domain mediates interaction with JAKMIP1.
Cellular localizationEndomembrane system. Wholly intracellular, possibly membrane associated.
- Information by UniProt
- JAK 1 antibody
- JAK 1A antibody
- JAK 1B antibody
ICC/IF image of ab47435 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47435, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-JAK1 antibody (ab47435) at 1/500 dilution
Lane 1 : MCF7 cell extract treated with EGF
Lane 2 : MCF7 cell extract treated with EGF with peptide
Predicted band size: 132 kDa
Immunohistochemical staining of paraffin embedded human breast carcinoma tissue using ab47435 at dilution of 1/50-1/100. Right panel treated with peptide and left panel untreated
This product has been referenced in:
- Chen X et al. MicroRNA-214 inhibits the proliferation and invasion of lung carcinoma cells by targeting JAK1. Am J Transl Res 10:1164-1171 (2018). Read more (PubMed: 29736209) »
- Zhao L et al. Long non-coding RNA HULC promotes UVB-induced injury by up-regulation of BNIP3 in keratinocytes. Biomed Pharmacother 104:672-678 (2018). WB ; Human . Read more (PubMed: 29803927) »