- Datasheet
- Specific References (6)
- Protocols
Overview
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Product nameAnti-JAK1 antibody
See all JAK1 primary antibodies -
DescriptionRabbit polyclonal to JAK1
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Host speciesRabbit
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SpecificityDetects endogenous levels of JAK1 protein
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Tested applicationsSuitable for: ICC/IF, IHC-P, ELISA, WBmore details
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Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
Synthetic non - phosphopeptide (Human) from around the phosphorylation site of Tyrosine 1022
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Positive control
- Human breast carcinoma tissue. Extracts from MCF7 cells.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesAffinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab47435 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 1 µg/ml. | |
| IHC-P | Use at an assay dependent concentration. | |
| ELISA | 1/10000. | |
| WB | 1/500 - 1/1000. Detects a band of approximately 132 kDa (predicted molecular weight: 132 kDa). |
Target
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FunctionTyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway. Kinase partner for the interleukin (IL)-2 receptor.
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Tissue specificityExpressed at higher levels in primary colon tumors than in normal colon tissue. The expression level in metastatic colon tumors is comparable to the expression level in normal colon tissue.
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Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.
Contains 1 FERM domain.
Contains 1 protein kinase domain.
Contains 1 SH2 domain. -
DomainPossesses two phosphotransferase domains. The second one probably contains the catalytic domain (By similarity), while the presence of slight differences suggest a different role for domain 1.
The FERM domain mediates interaction with JAKMIP1. -
Cellular localizationEndomembrane system. Wholly intracellular, possibly membrane associated.
- Information by UniProt
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Database links
- Entrez Gene: 3716 Human
- Entrez Gene: 16451 Mouse
- Entrez Gene: 84598 Rat
- Omim: 147795 Human
- SwissProt: P23458 Human
- SwissProt: P52332 Mouse
- Unigene: 207538 Human
- Unigene: 289657 Mouse
see all -
Alternative names
- JAK 1 antibody
- JAK 1A antibody
- JAK 1B antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JAK1 antibody (ab47435)Immunohistochemical staining of paraffin embedded human breast carcinoma tissue using ab47435 at dilution of 1/50-1/100. Right panel treated with peptide and left panel untreated -
Western blot - Anti-JAK1 antibody (ab47435)All lanes : Anti-JAK1 antibody (ab47435) at 1/500 dilution
Lane 1 : MCF7 cell extract treated with EGF
Lane 2 : MCF7 cell extract treated with EGF with peptide
Predicted band size: 132 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-JAK1 antibody (ab47435)ICC/IF image of ab47435 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47435, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
References
This product has been referenced in:
- Chen X et al. MicroRNA-214 inhibits the proliferation and invasion of lung carcinoma cells by targeting JAK1. Am J Transl Res 10:1164-1171 (2018). Read more (PubMed: 29736209) »
- Zhao L et al. Long non-coding RNA HULC promotes UVB-induced injury by up-regulation of BNIP3 in keratinocytes. Biomed Pharmacother 104:672-678 (2018). WB ; Human . Read more (PubMed: 29803927) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"