Overview

  • Product name
  • Description
    Rabbit polyclonal to JAK1
  • Host species
    Rabbit
  • Specificity
    Detects endogenous levels of JAK1 protein
  • Tested applications
    Suitable for: ICC/IF, IHC-P, ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic non - phosphopeptide (Human) from around the phosphorylation site of Tyrosine 1022

  • Positive control
    • Human breast carcinoma tissue. Extracts from MCF7 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab47435 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
IHC-P Use at an assay dependent concentration.
ELISA 1/10000.
WB 1/500 - 1/1000. Detects a band of approximately 132 kDa (predicted molecular weight: 132 kDa).

Target

  • Function
    Tyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway. Kinase partner for the interleukin (IL)-2 receptor.
  • Tissue specificity
    Expressed at higher levels in primary colon tumors than in normal colon tissue. The expression level in metastatic colon tumors is comparable to the expression level in normal colon tissue.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.
    Contains 1 FERM domain.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
  • Domain
    Possesses two phosphotransferase domains. The second one probably contains the catalytic domain (By similarity), while the presence of slight differences suggest a different role for domain 1.
    The FERM domain mediates interaction with JAKMIP1.
  • Cellular localization
    Endomembrane system. Wholly intracellular, possibly membrane associated.
  • Information by UniProt
  • Database links
  • Alternative names
    • JAK 1 antibody
    • JAK 1A antibody
    • JAK 1B antibody
    • JAK-1 antibody
    • JAK1 antibody
    • JAK1_HUMAN antibody
    • JAK1A antibody
    • JAK1B antibody
    • Janus kinase 1 (a protein tyrosine kinase) antibody
    • Janus kinase 1 antibody
    • JTK3 antibody
    • Tyrosine protein kinase JAK 1 antibody
    • Tyrosine protein kinase JAK1 antibody
    • Tyrosine-protein kinase JAK1 antibody
    see all

Images

  • All lanes : Anti-JAK1 antibody (ab47435) at 1/500 dilution

    Lane 1 : MCF7 cell extract treated with EGF
    Lane 2 : MCF7 cell extract treated with EGF with peptide

    Predicted band size: 132 kDa

  • Immunohistochemical staining of paraffin embedded human breast carcinoma tissue using ab47435 at dilution of 1/50-1/100. Right panel treated with peptide and left panel untreated
  • ICC/IF image of ab47435 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47435, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Chen X  et al. MicroRNA-214 inhibits the proliferation and invasion of lung carcinoma cells by targeting JAK1. Am J Transl Res 10:1164-1171 (2018). Read more (PubMed: 29736209) »
  • Zhao L  et al. Long non-coding RNA HULC promotes UVB-induced injury by up-regulation of BNIP3 in keratinocytes. Biomed Pharmacother 104:672-678 (2018). WB ; Human . Read more (PubMed: 29803927) »
See all 8 Publications for this product

Customer reviews and Q&As

Answer

Please accept my apologies for you having trouble with ab47435. The anti-JAK1 antibody is covered under our Abpromise, which states that we guarantee all of our antibodies for 6 months to work as stated on the datasheet. In the case of ab47435, it is guaranteed to work in ICC and WB on human samples. If the antibody fails to work as guaranteed if we cannot help resolve these issues, then will gladly replace the antibody or process a refund. I would be very happy to help you try and troubleshoot some of the issues you are having. To do this could you please provide me some information on the protocols that you have tried? For the western blot, are you seeing no bands when you develop your blot? Or are you seeing multiple bands, or high background? If you are seeing nothing, what primary antibody concentrations have you tried and what is the total protein concentration that you are loading on your gel? Also do you have a positive control on your gel? I ask as may be either increasing the antibody concentration or the protein concentration loaded onto the gel may help resolve seeing no bands. Incubating the antibody O/N at 4C would also be beneficial. If you are having issues with high background on your membrane, then if you are using milk as your blocking reagent, switching to BSA may help and vice-versa. For the ICC, what tissue/cell samples are you using? Again depending on whether you are seeing no signal or high background, either increasing the antibody concentration or increasing the blocking (or changing the blocking reagent) could prove helpful. As I mentioned previously, this antibody is covered under our Abpromise and so if we cannot resolve these issues I would be happy to send you another JAK1 antibody or a refund. I look forward to your repl

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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