Recombinant Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] (ab138005)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1899(2)] to JAK1 (phospho Y1022 + Y1023)
- Suitable for: WB, IHC-P, Dot blot, ELISA
- Reacts with: Human
Overview
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Product name
Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)]
See all JAK1 primary antibodies -
Description
Rabbit monoclonal [EPR1899(2)] to JAK1 (phospho Y1022 + Y1023) -
Host species
Rabbit -
Specificity
According to our ELISA result, this antibody preferentially recognizes JAK1 with phospho Y1023, but also recognizes JAK1 with phospho Y1022 if high amount peptides were coated. -
Tested applications
Suitable for: WB, IHC-P, Dot blot, ELISAmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human JAK1 aa 1000-1100. The exact sequence is proprietary.
Database link: P23458 -
Positive control
- WB: Ramos whole cell lysate. IHC-P: Human colon adenocarcinoma and liver carcinoma tissue.
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General notes
Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Dissociation constant (KD)
KD = 1.99 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.21% BSA, 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR1899(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab138005 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 133 kDa.
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Dot blot |
Use at an assay dependent concentration.
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ELISA |
Use at an assay dependent concentration.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 133 kDa. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Dot blot
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent concentration. |
Target
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Function
Tyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway. Kinase partner for the interleukin (IL)-2 receptor. -
Tissue specificity
Expressed at higher levels in primary colon tumors than in normal colon tissue. The expression level in metastatic colon tumors is comparable to the expression level in normal colon tissue. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.
Contains 1 FERM domain.
Contains 1 protein kinase domain.
Contains 1 SH2 domain. -
Domain
Possesses two phosphotransferase domains. The second one probably contains the catalytic domain (By similarity), while the presence of slight differences suggest a different role for domain 1.
The FERM domain mediates interaction with JAKMIP1. -
Cellular localization
Endomembrane system. Wholly intracellular, possibly membrane associated. - Information by UniProt
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Database links
- Entrez Gene: 3716 Human
- Omim: 147795 Human
- SwissProt: P23458 Human
- Unigene: 207538 Human
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Alternative names
- JAK 1 antibody
- JAK 1A antibody
- JAK 1B antibody
see all
Images
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All lanes : Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] (ab138005) at 1/5000 dilution
Lane 1 : Untreated Ramos whole cell lysate
Lane 2 : Ramos treated with 1mM pervanadate for 30 minutes whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 133 kDa
Observed band size: 133 kDaBlocking and diluting buffer 5% NFDM/TBST
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] (ab138005)
Immunohistochemical analysis of paraffin-embedded human liver carcinoma sections labelling JAK1 (phospho Y1022 + Y1023) with purified ab138005 at dilution of 1/50. The secondary antibody used was ImmunoHistoProbe HRP Polymer for Rabbit IgG at dilution of 1:0. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0.
PBS was used instead of the primary antibody as the negative control and is shown in the inset.
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Primary antibody: ab138005 at a 1/1000 dilution (unpurified).
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at a 1/100,000 dilution.
Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
Lane 1: JAK1 (pY1022) phospho peptide
Lane 2: JAK1 (pY1023) phospho peptide
Lane 3: JAK1 (pY1022/pY1023) phospho peptide
Lane 4: JAK1 non-phospho peptide
Exposure time: 3 mins.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] (ab138005)
Immunohistochemical analysis of paraffin embedded human colon adenocarcinoma tissue labelling JAK1 with unpurified ab138005 antibody at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] (ab138005) at 1/1000 dilution (unpurified)
Lane 1 : Ramos cell lysates
Lane 2 : Ramos cell lysates, treated with pervanadate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 133 kDa -
Antigen:
- JAK1 (pY1022) phospho peptide
- JAK1 (pY1023) phospho peptide
- JAK1 non-phospho peptide
Antigen concentration: 0~1000ng/ml
Primary antibody concentration range: 1000ng/ml
Secondary antibody: Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), 1/2500 dilution
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (19)
ab138005 has been referenced in 19 publications.
- Liu G et al. Circ_0038467 regulates lipopolysaccharide-induced inflammatory injury in human bronchial epithelial cells through sponging miR-338-3p. Thorac Cancer 11:1297-1308 (2020). PubMed: 32181994
- Gao F et al. MALAT1 is involved in type I IFNs-mediated systemic lupus erythematosus by up-regulating OAS2, OAS3, and OASL. Braz J Med Biol Res 53:e9292 (2020). PubMed: 32321151
- Bradley JR et al. Tumor necrosis factor receptor-2 signaling pathways promote survival of cancer stem-like CD133+ cells in clear cell renal carcinoma. FASEB Bioadv 2:126-144 (2020). PubMed: 32123862
- Wang S et al. Syndecan-1 suppresses cell growth and migration via blocking JAK1/STAT3 and Ras/Raf/MEK/ERK pathways in human colorectal carcinoma cells. BMC Cancer 19:1160 (2019). PubMed: 31783811
- Li X et al. Silence of MEG3 intensifies lipopolysaccharide-stimulated damage of human lung cells through modulating miR-4262. Artif Cells Nanomed Biotechnol 47:2369-2378 (2019). PubMed: 31184231