Recombinant
RabMAb

Recombinant Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] (ab138005)

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Overview

  • Product name

    Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)]
    See all JAK1 primary antibodies

  • Description

    Rabbit monoclonal [EPR1899(2)] to JAK1 (phospho Y1022 + Y1023)

  • Host species

    Rabbit

  • Specificity

    According to our ELISA result, this antibody preferentially recognizes JAK1 with phospho Y1023, but also recognizes JAK1 with phospho Y1022 if high amount peptides were coated.

  • Tested applications

    Suitable for: WB, IHC-P, Dot blot, ELISAmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human JAK1 aa 1000-1100. The exact sequence is proprietary.
    Database link: P23458

  • Positive control

    • WB: Ramos whole cell lysate. IHC-P: Human colon adenocarcinoma and liver carcinoma tissue.

  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab138005 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 133 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.
Dot blot Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

Target

  • Function

    Tyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway. Kinase partner for the interleukin (IL)-2 receptor.

  • Tissue specificity

    Expressed at higher levels in primary colon tumors than in normal colon tissue. The expression level in metastatic colon tumors is comparable to the expression level in normal colon tissue.

  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.
    Contains 1 FERM domain.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.

  • Domain

    Possesses two phosphotransferase domains. The second one probably contains the catalytic domain (By similarity), while the presence of slight differences suggest a different role for domain 1.
    The FERM domain mediates interaction with JAKMIP1.

  • Cellular localization

    Endomembrane system. Wholly intracellular, possibly membrane associated.
  • Information by UniProt

  • Database links

    • Alternative names

      • JAK 1 antibody
      • JAK 1A antibody
      • JAK 1B antibody
      • JAK-1 antibody
      • JAK1 antibody
      • JAK1_HUMAN antibody
      • JAK1A antibody
      • JAK1B antibody
      • Janus kinase 1 (a protein tyrosine kinase) antibody
      • Janus kinase 1 antibody
      • JTK3 antibody
      • Tyrosine protein kinase JAK 1 antibody
      • Tyrosine protein kinase JAK1 antibody
      • Tyrosine-protein kinase JAK1 antibody
      see all

    Images

    • Western blot - Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] (ab138005)
      Western blot - Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] (ab138005)
      All lanes : Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] (ab138005) at 1/5000 dilution

      Lane 1 : Untreated Ramos whole cell lysate
      Lane 2 : Ramos treated with 1mM pervanadate for 30 minutes whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 133 kDa
      Observed band size: 133 kDa



      Blocking and diluting buffer 5% NFDM/TBST

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] (ab138005)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] (ab138005)

      Immunohistochemical analysis of paraffin-embedded human liver carcinoma sections labelling JAK1 (phospho Y1022 + Y1023) with purified ab138005 at dilution of 1/50. The secondary antibody used was ImmunoHistoProbe HRP Polymer for Rabbit IgG at dilution of 1:0. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0.

      PBS was used instead of the primary antibody as the negative control and is shown in the inset.

    • Dot Blot - Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] (ab138005)
      Dot Blot - Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] (ab138005)

      Primary antibody: ab138005 at a 1/1000 dilution (unpurified).

      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at a 1/100,000 dilution.

      Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

      Lane 1: JAK1 (pY1022) phospho peptide

      Lane 2: JAK1 (pY1023) phospho peptide

      Lane 3: JAK1 (pY1022/pY1023) phospho peptide

      Lane 4: JAK1 non-phospho peptide

      Exposure time: 3 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] (ab138005)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] (ab138005)

      Immunohistochemical analysis of paraffin embedded human colon adenocarcinoma tissue labelling JAK1 with unpurified ab138005 antibody at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Western blot - Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] (ab138005)
      Western blot - Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] (ab138005)
      All lanes : Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] (ab138005) at 1/1000 dilution (unpurified)

      Lane 1 : Ramos cell lysates
      Lane 2 : Ramos cell lysates, treated with pervanadate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 133 kDa

    • ELISA - Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] (ab138005)
      ELISA - Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] (ab138005)

      Antigen:

      1. JAK1 (pY1022) phospho peptide
      2. JAK1 (pY1023) phospho peptide
      3. JAK1 non-phospho peptide

      Antigen concentration: 0~1000ng/ml

      Primary antibody concentration range: 1000ng/ml

      Secondary antibody: Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), 1/2500 dilution

       

    • Other - Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] (ab138005)
      Other - Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] (ab138005)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    See all 12 Publications for this product

    Publishing research using ab138005? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Show All Reviews Q&A
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    Question

    Could I get the details of your pervanadate treatment in the WB image on the datasheet? The concentration, incubation time, and method of reconstituting would be very helpful.

    Read More
    Answer

    Thank you for contacting us. Please find below the notes on the image with pervanadate.


    Prepare 500mM stock Sodium Orthovanadate in PBS. To make
    100X Pervanadate right before experiment, add 400ul 500mM Sodium Orthovanadate to 1560ul H2O and then 40ul H2O2(Fisher #H325-100), vortex and incubate at RT for 5-10min.

    Cells are serum-starved for overnight, and then treated with freshly made Pervanadate for 20min at 37 degrees C.


    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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