Recombinant
RabMAb

Recombinant Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] (ab138005)

Overview

  • Product name
    Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)]
    See all JAK1 primary antibodies
  • Description
    Rabbit monoclonal [EPR1899(2)] to JAK1 (phospho Y1022 + Y1023)
  • Host species
    Rabbit
  • Specificity
    According to our ELISA result, this antibody preferentially recognizes JAK1 with phospho Y1023, but also recognizes JAK1 with phospho Y1022 if high amount peptides were coated.
  • Tested applications
    Suitable for: WB, IHC-P, Dot blot, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human JAK1. The exact sequence is proprietary.

  • Positive control
    • Ramos cell lysates, treated with pervanadate; Human colon adenocarcinoma tissue, Human liver carcinoma.
  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab138005 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 133 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

Dot blot Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

Target

  • Function
    Tyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway. Kinase partner for the interleukin (IL)-2 receptor.
  • Tissue specificity
    Expressed at higher levels in primary colon tumors than in normal colon tissue. The expression level in metastatic colon tumors is comparable to the expression level in normal colon tissue.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.
    Contains 1 FERM domain.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
  • Domain
    Possesses two phosphotransferase domains. The second one probably contains the catalytic domain (By similarity), while the presence of slight differences suggest a different role for domain 1.
    The FERM domain mediates interaction with JAKMIP1.
  • Cellular localization
    Endomembrane system. Wholly intracellular, possibly membrane associated.
  • Information by UniProt
  • Database links
  • Alternative names
    • JAK 1 antibody
    • JAK 1A antibody
    • JAK 1B antibody
    • JAK-1 antibody
    • JAK1 antibody
    • JAK1_HUMAN antibody
    • JAK1A antibody
    • JAK1B antibody
    • Janus kinase 1 (a protein tyrosine kinase) antibody
    • Janus kinase 1 antibody
    • JTK3 antibody
    • Tyrosine protein kinase JAK 1 antibody
    • Tyrosine protein kinase JAK1 antibody
    • Tyrosine-protein kinase JAK1 antibody
    see all

Images

  • All lanes : Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] (ab138005) at 1/5000 dilution

    Lane 1 : Untreated Ramos whole cell lysate
    Lane 2 : Ramos treated with 1mM pervandate for 30 minutes whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 133 kDa
    Observed band size: 133 kDa



    Blocking and Diluting buffer 5% NFDM/TBST
  • Primary antibody: 1/1000 (unpurified)

    Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051)

    Secondary ab dilution: 1/100,000

    Blocking buffer and concentration: 5% NFDM/TBST

    Diluting buffer and concentration: 5% NFDM /TBST

    Lane 1: JAK1 (pY1022) phospho peptide

    Lane 2: JAK1 (pY1023) phospho peptide

    Lane 3: JAK1 (pY1022/pY1023) phospho peptide

    Lane 4: JAK1 non-phospho peptide

    Exposure time: 3 minutes.

  • Immunohistochemical analysis of paraffin-embedded human liver carcinoma sections labelling JAK1 (phospho Y1022 + Y1023) with purified ab138005 at dilution of 1/50. The secondary antibody used was ImmunoHistoProbe HRP Polymer for Rabbit IgG at dilution of 1:0. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.

  • Immunohistochemical analysis of paraffin embedded Human colon adenocarcinoma tissue labelling JAK1 with unpurified ab138005 antibody at 1/50 dilution.

  • All lanes : Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] (ab138005) at 1/1000 dilution (unpurified)

    Lane 1 : Ramos cell lysates
    Lane 2 : Ramos cell lysates, treated with pervanadate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 133 kDa

  • Antigen:

    1. JAK1 (pY1022) phospho peptide
    2. JAK1 (pY1023) phospho peptide
    3. JAK1 non-phospho peptide

    Antigen concentration: 0~1000ng/ml

    Primary antibody concentration range: 1000ng/ml

    Secondary antibody: Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), 1/2500 dilution

     

  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

References

This product has been referenced in:
  • Jiang J  et al. Polydatin inhibits hepatocellular carcinoma via the AKT/STAT3-FOXO1 signaling pathway. Oncol Lett 17:4505-4513 (2019). Read more (PubMed: 30944640) »
  • Zhu J  et al. Blueberry, combined with probiotics, alleviates non-alcoholic fatty liver disease via IL-22-mediated JAK1/STAT3/BAX signaling. Food Funct 9:6298-6306 (2018). Read more (PubMed: 30411754) »
See all 9 Publications for this product

Customer reviews and Q&As

Answer

Thank you for contacting us. Please find below the notes on the image with pervanadate.


Prepare 500mM stock Sodium Orthovanadate in PBS. To make
100X Pervanadate right before experiment, add 400ul 500mM Sodium Orthovanadate to 1560ul H2O and then 40ul H2O2(Fisher #H325-100), vortex and incubate at RT for 5-10min.

Cells are serum-starved for overnight, and then treated with freshly made Pervanadate for 20min at 37 degrees C.


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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