Recombinant Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] - BSA and Azide free (ab203784)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameAnti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] - BSA and Azide free
See all JAK1 primary antibodies -
DescriptionRabbit monoclonal [EPR1899(2)] to JAK1 (phospho Y1022 + Y1023) - BSA and Azide free
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Host speciesRabbit
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Tested applicationsSuitable for: ELISA, Dot blot, WB, IHC-Pmore details
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Species reactivityReacts with: Human
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Immunogen
A phospho specific peptide corresponding to residues surrounding both Tyrosine 1022 and 1023 of Human JAK1.
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Positive control
- Ramos cell lysates, treated with pervanadate; Human colon adenocarcinoma tissue.
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General notes
The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®).
Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at 4°C (stable for up to 12 months). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Dissociation constant (KD)KD = 1.99 x 10 -11 M Learn more about KD
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Storage bufferConstituent: PBS
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Concentration information loading... -
PurityAffinity purified
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ClonalityMonoclonal
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Clone numberEPR1899(2)
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab203784 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | Use at an assay dependent concentration. | |
| Dot blot | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. Predicted molecular weight: 133 kDa. | |
| IHC-P | Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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FunctionTyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway. Kinase partner for the interleukin (IL)-2 receptor.
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Tissue specificityExpressed at higher levels in primary colon tumors than in normal colon tissue. The expression level in metastatic colon tumors is comparable to the expression level in normal colon tissue.
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Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.
Contains 1 FERM domain.
Contains 1 protein kinase domain.
Contains 1 SH2 domain. -
DomainPossesses two phosphotransferase domains. The second one probably contains the catalytic domain (By similarity), while the presence of slight differences suggest a different role for domain 1.
The FERM domain mediates interaction with JAKMIP1. -
Cellular localizationEndomembrane system. Wholly intracellular, possibly membrane associated.
- Information by UniProt
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Database links
- Entrez Gene: 3716 Human
- Omim: 147795 Human
- SwissProt: P23458 Human
- Unigene: 207538 Human
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Alternative names
- JAK 1 antibody
- JAK 1A antibody
- JAK 1B antibody
see all
Images
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![Dot Blot - Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] - BSA and Azide free (ab203784)](https://www.abcam.com/ps/products/203/ab203784/Images/ab203784-5-ab138005271809ab138005.jpg)
Dot Blot - Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] - BSA and Azide free (ab203784)Primary antibody: 1/1000 (unpurified)
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051)
Secondary ab dilution: 1/100,000
Blocking buffer and concentration: 5% NFDM/TBST
Diluting buffer and concentration: 5% NFDM /TBST
Lane 1: JAK1 (pY1022) phospho peptide
Lane 2: JAK1 (pY1023) phospho peptide
Lane 3: JAK1 (pY1022/pY1023) phospho peptide
Lane 4: JAK1 non-phospho peptide
Exposure time: 3 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab138005).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] - BSA and Azide free (ab203784)](https://www.abcam.com/ps/products/203/ab203784/Images/ab203784-4-ab1380058ab138005IHC1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] - BSA and Azide free (ab203784)Immunohistochemical analysis of paraffin-embedded human liver carcinoma sections labelling JAK1 (phospho Y1022 + Y1023) with purified ab138005 at dilution of 1/50. The secondary antibody used was ImmunoHistoProbe HRP Polymer for Rabbit IgG at dilution of 1:0. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab138005).
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![ELISA - Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] - BSA and Azide free (ab203784)](https://www.abcam.com/ps/products/203/ab203784/Images/ab203784-3-ab138005257265ab138005ELISA.jpg)
ELISA - Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] - BSA and Azide free (ab203784)Antigen:
- JAK1 (pY1022) phospho peptide
- JAK1 (pY1023) phospho peptide
- JAK1 non-phospho peptide
Antigen concentration: 0~1000ng/ml
Primary antibody concentration range: 1000ng/ml
Secondary antibody: Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), 1/2500 dilution
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab138005).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] - BSA and Azide free (ab203784)](https://www.abcam.com/ps/products/203/ab203784/Images/ab203784-2-JAK1Primaryantibodiesab1380052.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] - BSA and Azide free (ab203784) -
![Other - Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] - BSA and Azide free (ab203784)](https://www.abcam.com/ps/products/203/ab203784/Images/ab203784-1-JAK1Primaryantibodiesab1380053.jpg)
Other - Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] - BSA and Azide free (ab203784)Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KDThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab138005).
Datasheets and documents
References
This product has been referenced in:
- Liu W et al. Epigallocatechin-3-gallate ameliorates seawater aspiration-induced acute lung injury via regulating inflammatory cytokines and inhibiting JAK/STAT1 pathway in rats. Mediators Inflamm 2014:612593 (2014). WB ; Rat . Read more (PubMed: 24692852) »
