Recombinant Anti-JAK1 (phospho Y1034 + Y1035) antibody [EPR1899(2)] - BSA and Azide free (ab203784)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1899(2)] to JAK1 (phospho Y1034 + Y1035) - BSA and Azide free
- Suitable for: ELISA, Dot blot, WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-JAK1 (phospho Y1034 + Y1035) antibody [EPR1899(2)] - BSA and Azide free
See all JAK1 primary antibodies -
Description
Rabbit monoclonal [EPR1899(2)] to JAK1 (phospho Y1034 + Y1035) - BSA and Azide free -
Host species
Rabbit -
Specificity
According to our ELISA result, this antibody preferentially recognizes JAK1 with phospho Y1035, but also recognizes JAK1 with phospho Y1034 if high amount peptides were coated.
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Tested applications
Suitable for: ELISA, Dot blot, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Ramos treated with 1mM pervanadate for 30 minutes whole cell lysate. IHC-P: Human colon adenocarcinoma and liver carcinoma tissue. Dot: JAK1 (pY1034), JAK1 (pY1035) and JAK1 (pY1034/pY1035) phospho peptides. ELISA: JAK1 (pY1034), JAK1 (pY1035) and JAK1 non-phospho peptides.
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General notes
ab203784 is the carrier-free version of ab138005.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Dissociation constant (KD)
KD = 1.99 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR1899(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-JAK1 (phospho Y1034 + Y1035) antibody [EPR1899(2)] (ab138005)
- HRP Anti-JAK1 (phospho Y1034 + Y1035) antibody [EPR1899(2)] (ab215338)
- Alexa Fluor® 488 Anti-JAK1 (phospho Y1034 + Y1035) antibody [EPR1899(2)] (ab310950)
- Alexa Fluor® 647 Anti-JAK1 (phospho Y1034 + Y1035) antibody [EPR1899(2)] (ab311068)
- Alexa Fluor® 568 Anti-JAK1 (phospho Y1034 + Y1035) antibody [EPR1899(2)] (ab312925)
- Alexa Fluor® 555 Anti-JAK1 (phospho Y1034 + Y1035) antibody [EPR1899(2)] (ab313138)
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab203784 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent concentration.
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Dot blot |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 133 kDa.
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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ELISA
Use at an assay dependent concentration. |
Dot blot
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 133 kDa. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Tyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway. Kinase partner for the interleukin (IL)-2 receptor. -
Tissue specificity
Expressed at higher levels in primary colon tumors than in normal colon tissue. The expression level in metastatic colon tumors is comparable to the expression level in normal colon tissue. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.
Contains 1 FERM domain.
Contains 1 protein kinase domain.
Contains 1 SH2 domain. -
Domain
Possesses two phosphotransferase domains. The second one probably contains the catalytic domain (By similarity), while the presence of slight differences suggest a different role for domain 1.
The FERM domain mediates interaction with JAKMIP1. -
Cellular localization
Endomembrane system. Wholly intracellular, possibly membrane associated. - Information by UniProt
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Database links
- Entrez Gene: 3716 Human
- Omim: 147795 Human
- SwissProt: P23458 Human
- Unigene: 207538 Human
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Alternative names
- JAK 1 antibody
- JAK 1A antibody
- JAK 1B antibody
see all
Images
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All lanes : Anti-JAK1 (phospho Y1034 + Y1035) antibody [EPR1899(2)] (ab138005) at 1/500 dilution
Lane 1 : Untreated Ramos whole cell lysate
Lane 2 : Ramos treated with 1mM pervanadate for 30 minutes whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 133 kDa
Observed band size: 133 kDaBlocking and diluting buffer 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab138005).
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Immunohistochemical analysis of paraffin-embedded human liver carcinoma sections labelling JAK1 (phospho Y1034 + Y1035) with purified ab138005 at dilution of 1/50. The secondary antibody used was ImmunoHistoProbe HRP Polymer for Rabbit IgG at dilution of 1:0. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab138005).
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Primary antibody: 1/1000 (unpurified)
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051)
Secondary ab dilution: 1/100,000
Blocking buffer and concentration: 5% NFDM/TBST
Diluting buffer and concentration: 5% NFDM /TBST
Lane 1: JAK1 (pY1034) phospho peptide
Lane 2: JAK1 (pY1035) phospho peptide
Lane 3: JAK1 (pY1034/pY1035) phospho peptide
Lane 4: JAK1 non-phospho peptide
Exposure time: 3 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab138005).
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Immunohistochemical analysis of paraffin embedded Human colon adenocarcinoma tissue labelling JAK1 with unpurified ab138005 antibody at 1/50 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab138005).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Antigen:
- JAK1 (pY1034) phospho peptide
- JAK1 (pY1035) phospho peptide
- JAK1 non-phospho peptide
Antigen concentration: 0~1000ng/ml
Primary antibody concentration range: 1000ng/ml
Secondary antibody: Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), 1/2500 dilution
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab138005).
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Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KDThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab138005).
Protocols
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (3)
ab203784 has been referenced in 3 publications.
- Li T et al. Overexpression of miRNA-93-5p Promotes Proliferation and Migration of Bladder Urothelial Carcinoma via Inhibition of KLF9. Comput Math Methods Med 2022:8911343 (2022). PubMed: 35495878
- Wang X et al. Silence of lncRNA ANRIL represses cell growth and promotes apoptosis in retinoblastoma cells through regulating miR-99a and c-Myc. Artif Cells Nanomed Biotechnol 47:2265-2273 (2019). PubMed: 31184221
- Liu W et al. Epigallocatechin-3-gallate ameliorates seawater aspiration-induced acute lung injury via regulating inflammatory cytokines and inhibiting JAK/STAT1 pathway in rats. Mediators Inflamm 2014:612593 (2014). WB ; Rat . PubMed: 24692852