Recombinant
RabMAb

Recombinant Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] (HRP) (ab215338)

Overview

  • Product name
    Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] (HRP)
    See all JAK1 primary antibodies
  • Description
    Rabbit monoclonal [EPR1899(2)] to JAK1 (phospho Y1022 + Y1023) (HRP)
  • Host species
    Rabbit
  • Conjugation
    HRP
  • Tested applications
    Suitable for: Dot blot, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human JAK1 aa 1000-1100 (phospho Y1022 + Y1023). The exact sequence is proprietary.
    Database link: P23458

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab215338 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot 1/1000.
WB 1/1000. Detects a band of approximately 133 kDa (predicted molecular weight: 133 kDa).

Target

  • Function
    Tyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway. Kinase partner for the interleukin (IL)-2 receptor.
  • Tissue specificity
    Expressed at higher levels in primary colon tumors than in normal colon tissue. The expression level in metastatic colon tumors is comparable to the expression level in normal colon tissue.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.
    Contains 1 FERM domain.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
  • Domain
    Possesses two phosphotransferase domains. The second one probably contains the catalytic domain (By similarity), while the presence of slight differences suggest a different role for domain 1.
    The FERM domain mediates interaction with JAKMIP1.
  • Cellular localization
    Endomembrane system. Wholly intracellular, possibly membrane associated.
  • Information by UniProt
  • Database links
  • Alternative names
    • JAK 1 antibody
    • JAK 1A antibody
    • JAK 1B antibody
    • JAK-1 antibody
    • JAK1 antibody
    • JAK1_HUMAN antibody
    • JAK1A antibody
    • JAK1B antibody
    • Janus kinase 1 (a protein tyrosine kinase) antibody
    • Janus kinase 1 antibody
    • JTK3 antibody
    • Tyrosine protein kinase JAK 1 antibody
    • Tyrosine protein kinase JAK1 antibody
    • Tyrosine-protein kinase JAK1 antibody
    see all

Images

  • All lanes : Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] (HRP) (ab215338) at 1/1000 dilution

    Lane 1 : Untreated Ramos cells
    Lanes 2-3 : Ramos cells treated with pervanadateat at 1mM for 30min

    Predicted band size: 133 kDa
    Observed band size: 133 kDa


    Exposure time: 5 seconds
  • Dot blot analysis of JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] (HRP) (ab215338) at 1/1000 dilution.
    Lane 1: JAK1 (phospho Y1022) phospho peptide.
    Lane 2: JAK1 (phospho Y1023) phospho peptide.
    Lane 3: JAK1 (phospho Y1022 + Y1023) phospho peptide.
    Lane 4: JAK1 non phospho peptide.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST. Exposure time: 3 minutes.

References

This product has been referenced in:
  • Li Y  et al. Curcumin inhibits proliferation, migration, invasion and promotes apoptosis of retinoblastoma cell lines through modulation of miR-99a and JAK/STAT pathway. BMC Cancer 18:1230 (2018). Read more (PubMed: 30526546) »
See 1 Publication for this product

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