Product nameAnti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] (HRP)
See all JAK1 primary antibodies
DescriptionRabbit monoclonal [EPR1899(2)] to JAK1 (phospho Y1022 + Y1023) (HRP)
Tested applicationsSuitable for: Dot blot, WBmore details
Species reactivityReacts with: Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human JAK1 aa 1000-1100 (phospho Y1022 + Y1023). The exact sequence is proprietary.
Database link: P23458
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.1% Proclin
Constituents: 1% BSA, 30% Glycerol, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab215338 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 133 kDa (predicted molecular weight: 133 kDa).|
FunctionTyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway. Kinase partner for the interleukin (IL)-2 receptor.
Tissue specificityExpressed at higher levels in primary colon tumors than in normal colon tissue. The expression level in metastatic colon tumors is comparable to the expression level in normal colon tissue.
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.
Contains 1 FERM domain.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
DomainPossesses two phosphotransferase domains. The second one probably contains the catalytic domain (By similarity), while the presence of slight differences suggest a different role for domain 1.
The FERM domain mediates interaction with JAKMIP1.
Cellular localizationEndomembrane system. Wholly intracellular, possibly membrane associated.
- Information by UniProt
- JAK 1 antibody
- JAK 1A antibody
- JAK 1B antibody
All lanes : Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] (HRP) (ab215338) at 1/1000 dilution
Lane 1 : Untreated Ramos cells
Lanes 2-3 : Ramos cells treated with pervanadateat at 1mM for 30min
Predicted band size: 133 kDa
Observed band size: 133 kDa
Exposure time: 5 seconds
Dot blot analysis of JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] (HRP) (ab215338) at 1/1000 dilution.
Lane 1: JAK1 (phospho Y1022) phospho peptide.
Lane 2: JAK1 (phospho Y1023) phospho peptide.
Lane 3: JAK1 (phospho Y1022 + Y1023) phospho peptide.
Lane 4: JAK1 non phospho peptide.
Blocking and diluting buffer and concentration: 5% NFDM/TBST. Exposure time: 3 minutes.