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  1. Link

    jak2-antibody-epr1082-ab108596.pdf

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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-JAK2 antibody [EPR108(2)] (ab108596)

  • Datasheet
  • SDS
Reviews (3) Submit a question References (95)

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Western blot - Anti-JAK2 antibody [EPR108(2)] (ab108596)
  • Immunocytochemistry/ Immunofluorescence - Anti-JAK2 antibody [EPR108(2)] (ab108596)
  • Western blot - Anti-JAK2 antibody [EPR108(2)] (ab108596)
  • Western blot - Anti-JAK2 antibody [EPR108(2)] (ab108596)
  • Immunocytochemistry/ Immunofluorescence - Anti-JAK2 antibody [EPR108(2)] (ab108596)
  • Immunocytochemistry/ Immunofluorescence - Anti-JAK2 antibody [EPR108(2)] (ab108596)
  • Immunoprecipitation - Anti-JAK2 antibody [EPR108(2)] (ab108596)
  • Anti-JAK2 antibody [EPR108(2)] (ab108596)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR108(2)] to JAK2
  • Suitable for: ICC/IF, WB, IP
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free HRP

You may also be interested in

Primary
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Anti-JAK2 (phospho Y1007 + Y1008) antibody [E132] - BSA and Azide free (ab219728)
ELISA
Product image
JAK2 ELISA Kit (ab253224)
Knockout
Product image
Human JAK2 knockout A549 cell line (ab267113)

View more associated products

Overview

  • Product name

    Anti-JAK2 antibody [EPR108(2)]
    See all JAK2 primary antibodies
  • Description

    Rabbit monoclonal [EPR108(2)] to JAK2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IPmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: A549, TF-1, K562, THP-1, Jurkat, NIH 3T3, Ramos, C6 and IM-9 cell lysates. ICC/IF: K562, Jurkat and Ramos cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR108(2)
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Kinases/Phosphatases
    • Other
    • Cell Biology
    • Cell Cycle
    • Cell Cycle Inhibitors
    • Other
    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Other
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • STATs
    • Microbiology
    • Organism
    • Virus
    • RNA Virus
    • ssRNA positive strand virus
    • SARS Coronavirus
    • Cancer
    • Cell cycle
    • Cell cycle inhibitors
    • Other
    • Cancer
    • Cell cycle
    • Kinases/phosphatases
    • Other
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Signal transducers
    • Neuroscience
    • Development

Associated products

  • Alternative Versions

    • Anti-JAK2 antibody [EPR108(2)] - BSA and Azide free (ab170718)
    • HRP Anti-JAK2 antibody [EPR108(2)] (ab200783)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human JAK2 knockout A549 cell line (ab267113)
  • KO cell lysates

    • Human JAK2 knockout A549 cell lysate (ab256963)
  • KO cell pellets

    • Human JAK2 knockout A549 cell pellet (ab278814)
  • Positive Controls

    • NIH/3T3 nuclear extract lysate (ab14874)
    • THP-1 nuclear extract lysate (ab14900)
    • Jurkat membrane extract lysate (ab30130)
    • Ramos whole cell lysate (ab3955)
  • Recombinant Protein

    • Recombinant human JAK2 protein (ab42619)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab108596 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF (1)
1/150.

For unpurified use at 1/100 - 1/500.

WB (2)
1/5000. Detects a band of approximately 130 kDa (predicted molecular weight: 131 kDa).

For unpurified use at 1/1000.

IP
1/80.
Notes
ICC/IF
1/150.

For unpurified use at 1/100 - 1/500.

WB
1/5000. Detects a band of approximately 130 kDa (predicted molecular weight: 131 kDa).

For unpurified use at 1/1000.

IP
1/80.
Application notes
Is unsuitable for IHC-P.

Target

  • Function

    Non-receptor tyrosine kinase involved in various processes such as cell cycle progression, apoptosis, mitotic recombination, genetic instability and histone modifications. In the cytoplasm, plays a pivotal role in signal transduction via its association with cytokine receptors, which constitutes an initiating step in signaling for many members of the cytokine receptor superfamily including the receptors for growth hormone (GHR), prolactin (PRLR), leptin (LEPR), erythropoietin (EPOR), granulocyte-macrophage colony-stimulating factor (CSF2), thrombopoietin (THPO) and multiple interleukins. Following stimulation with erythropoietin (EPO) during erythropoiesis, it is autophosphorylated and activated, leading to its association with erythropoietin receptor (EPOR) and tyrosine phosphorylation of residues in the EPOR cytoplasmic domain. Also involved in promoting the localization of EPOR to the plasma membrane. Also acts downstream of some G-protein coupled receptors. Plays a role in the control of body weight (By similarity). Mediates angiotensin-2-induced ARHGEF1 phosphorylation. In the nucleus, plays a key role in chromatin by specifically mediating phosphorylation of 'Tyr-41' of histone H3 (H3Y41ph), a specific tag that promotes exclusion of CBX5 (HP1 alpha) from chromatin.
  • Tissue specificity

    Expressed in blood, bone marrow and lymph node.
  • Involvement in disease

    Note=Chromosomal aberrations involving JAK2 are found in both chronic and acute forms of eosinophilic, lymphoblastic and myeloid leukemia. Translocation t(8;9)(p22;p24) with PCM1 links the protein kinase domain of JAK2 to the major portion of PCM1. Translocation t(9;12)(p24;p13) with ETV6.
    Defects in JAK2 are a cause of susceptibility to Budd-Chiari syndrome (BCS) [MIM:600880]. It is a syndrome caused by obstruction of hepatic venous outflow involving either the hepatic veins or the terminal segment of the inferior vena cava. Obstructions are generally caused by thrombosis and lead to hepatic congestion and ischemic necrosis. Clinical manifestations observed in the majority of patients include hepatomegaly, right upper quadrant pain and abdominal ascites. Budd-Chiari syndrome is associated with a combination of disease states including primary myeloproliferative syndromes and thrombophilia due to factor V Leiden, protein C deficiency and antithrombin III deficiency. Budd-Chiari syndrome is a rare but typical complication in patients with polycythemia vera.
    Defects in JAK2 are a cause of polycythemia vera (PV) [MIM:263300]. A myeloproliferative disorder characterized by abnormal proliferation of all hematopoietic bone marrow elements, erythroid hyperplasia, an absolute increase in total blood volume, but also by myeloid leukocytosis, thrombocytosis and splenomegaly.
    Defects in JAK2 gene may be a cause of essential thrombocythemia (ET) [MIM:187950]. ET is characterized by elevated platelet levels due to sustained proliferation of megakaryocytes, and frequently lead to thrombotic and haemorrhagic complications.
    Defects in JAK2 are a cause of myelofibrosis (MYELOF) [MIM:254450]. Myelofibrosis is a disorder characterized by replacement of the bone marrow by fibrous tissue, occurring in association with a myeloproliferative disorder. Clinical manifestations may include anemia, pallor, splenomegaly, hypermetabolic state, petechiae, ecchymosis, bleeding, lymphadenopathy, hepatomegaly, portal hypertension.
    Defects in JAK2 are a cause of acute myelogenous leukemia (AML) [MIM:601626]. AML is a malignant disease in which hematopoietic precursors are arrested in an early stage of development.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.
    Contains 1 FERM domain.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
  • Domain

    Possesses 2 protein kinase domains. The second one probably contains the catalytic domain, while the presence of slight differences suggest a different role for protein kinase 1.
  • Post-translational
    modifications

    Autophosphorylated, leading to regulate its activity. Leptin promotes phosphorylation on tyrosine residues, including phosphorylation on Tyr-813. Autophosphorylation on Tyr-119 in response to EPO down-regulates its kinase activity. Autophosphorylation on Tyr-868, Tyr-966 and Tyr-972 in response to growth hormone (GH) are required for maximal kinase activity.
  • Cellular localization

    Endomembrane system. Nucleus.
  • Target information above from: UniProt accession O60674 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3717 Human
    • Entrez Gene: 16452 Mouse
    • Entrez Gene: 24514 Rat
    • Omim: 147796 Human
    • SwissProt: O60674 Human
    • SwissProt: Q62120 Mouse
    • SwissProt: Q62689 Rat
    • Unigene: 656213 Human
    • Unigene: 275839 Mouse
    • Unigene: 18909 Rat
    see all
  • Alternative names

    • JAK 2 antibody
    • JAK-2 antibody
    • JAK2 antibody
    • JAK2_HUMAN antibody
    • Janus Activating Kinase 2 antibody
    • Janus kinase 2 (a protein tyrosine kinase) antibody
    • Janus kinase 2 antibody
    • JTK 10 antibody
    • JTK10 antibody
    • kinase Jak2 antibody
    • OTTHUMP00000043260 antibody
    • THCYT3 antibody
    • Tyrosine protein kinase JAK2 antibody
    • Tyrosine-protein kinase JAK2 antibody
    see all

Images

  • Western blot - Anti-JAK2 antibody [EPR108(2)] (ab108596)
    Western blot - Anti-JAK2 antibody [EPR108(2)] (ab108596)
    All lanes : Anti-JAK2 antibody [EPR108(2)] (ab108596) at 1/1000 dilution

    Lane 1 : Wild-Type A549 cell lysate
    Lane 2 : JAK2 knockout A549 cell lysate
    Lane 3 : K562 cell lysate
    Lane 4 : Daudi cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 131 kDa
    Observed band size: 131 kDa



    Lanes 1- 4: Merged signal (red and green). Green - ab108596 observed at 131 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

     ab108596 was shown to react with JAK2 in Wild-Type A549 cells in western blot. Loss of signal was observed when knockout cell line ab267113 (knockout cell lysate ab256963) was used. Wild-Type A549 and JAK2 knockout A549 cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab108596 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-JAK2 antibody [EPR108(2)] (ab108596)
    Immunocytochemistry/ Immunofluorescence - Anti-JAK2 antibody [EPR108(2)] (ab108596)

    Immunocytochemistry/ Immunofluorescence analysis of K562 (Human chronic myelogenous leukemia cell line from bone marrow) cells labeling JAK2 with purified ab108596 at 1/150 dilution (8.5μg/ml). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, a goat anti-rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1/1000 dilution. Ab195889, anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used as the counter stain at 1/200 (2.5 μg/ml). PBS instead of the primary antibody was the negative control. DAPI was used as a nuclear counterstain.

  • Western blot - Anti-JAK2 antibody [EPR108(2)] (ab108596)
    Western blot - Anti-JAK2 antibody [EPR108(2)] (ab108596)
    All lanes : Anti-JAK2 antibody [EPR108(2)] (ab108596) at 1/5000 dilution (purified)

    Lane 1 : TF-1 (Human bone marrow erythroleukemia cell line) whole cell lysate
    Lane 2 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
    Lane 3 : C6 (Rat glial tumour cell line) whole cell lysate
    Lane 4 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

    Predicted band size: 131 kDa
    Observed band size: 130 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  • Western blot - Anti-JAK2 antibody [EPR108(2)] (ab108596)
    Western blot - Anti-JAK2 antibody [EPR108(2)] (ab108596)
    All lanes : Anti-JAK2 antibody [EPR108(2)] (ab108596) at 1/1000 dilution (unpurified)

    Lane 1 : TF-1 cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : THP-1 cell lysate
    Lane 4 : Jurkat cell lysate
    Lane 5 : NIH3T3 cell lysate
    Lane 6 : IM-9 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 131 kDa
    Observed band size: 130 kDa why is the actual band size different from the predicted?

  • Immunocytochemistry/ Immunofluorescence - Anti-JAK2 antibody [EPR108(2)] (ab108596)
    Immunocytochemistry/ Immunofluorescence - Anti-JAK2 antibody [EPR108(2)] (ab108596)

    Immunocytochemistry/Immunofluorescence analysis of Jurkat (Human T cell leukemia cells from peripheral blood) cells labelling JAK2 with unpurified ab108596 at 1/300 (7.0 μg/mL). Cells were fixed with 4% Paraformaldehydeand permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000, 2 μg/mL) was used as the secondary antibody. Nuclei were stained with DAPI (blue).

    Confocal image showing nuclear and cytoplasmic staining on Jurkat cell line. 

  • Immunocytochemistry/ Immunofluorescence - Anti-JAK2 antibody [EPR108(2)] (ab108596)
    Immunocytochemistry/ Immunofluorescence - Anti-JAK2 antibody [EPR108(2)] (ab108596)

    Immunocytochemistry/Immunofluorescence analysis of Ramos (Human Burkitt's lymphoma) cells labelling JAK2 with unpurified 108596 at 1/300 (7.0 μg/mL). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000, 2 μg/mL) was used as the secondary antibody. Nuclei were stained with DAPI (blue).

    Confocal image showing nuclear and cytoplasmic staining on Ramos cell line.

  • Immunoprecipitation - Anti-JAK2 antibody [EPR108(2)] (ab108596)
    Immunoprecipitation - Anti-JAK2 antibody [EPR108(2)] (ab108596)

    ab108596 at 1/80 dilution (20 µg/mL) immunoprecipitating JAK2 in K562 (Human chronic myelogenous leukemia lymphoblast)  cell lysate.

    Lane 1 (input): K562(Human chronic myelogenous leukemia lymphoblast) whole cell lysate 10µg

    Lane 2 (+): K562 whole cell lysate, 350µg  + ab108596, 2µg

    Lane 3 (-): K562 cell lysate, 350µg  + rabbit IgG (ab172730), 2µg

    For western blotting, ab108596 at 1/500 dilution (3.0 µg/mL) and ab131366 VeriBlot for IP (HRP) was used at 1/1000.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Anti-JAK2 antibody [EPR108(2)] (ab108596)
    Anti-JAK2 antibody [EPR108(2)] (ab108596)

Protocols

  • Western blot protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (95)

Publishing research using ab108596? Please let us know so that we can cite the reference in this datasheet.

ab108596 has been referenced in 95 publications.

  • Bian G  et al. DGT, a novel heterocyclic diterpenoid, effectively suppresses psoriasis via inhibition of STAT3 phosphorylation. Br J Pharmacol 178:636-653 (2021). PubMed: 33140855
  • Wang R  et al. Amygdalin promotes the activity of T cells to suppress the progression of HBV-related hepatocellular carcinoma via the JAK2/STAT3 signaling pathway. BMC Infect Dis 21:56 (2021). PubMed: 33435880
  • Qu H  et al. HIF-3a affects preeclampsia development by regulating EVT growth via activation of the Flt-1/JAK/STAT signaling pathway in hypoxia. Mol Med Rep 23:N/A (2021). PubMed: 33215219
  • Li Y  et al. Potential effect of Maxing Shigan decoction against coronavirus disease 2019 (COVID-19) revealed by network pharmacology and experimental verification. J Ethnopharmacol 271:113854 (2021). PubMed: 33513419
  • Qu L  et al. Fraxetin Inhibits the Proliferation and Metastasis of Glioma Cells by Inactivating JAK2/STAT3 Signaling. Evid Based Complement Alternat Med 2021:5540139 (2021). PubMed: 33959183
View all Publications for this product

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-JAK2 antibody [EPR108(2)]

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (C42B Prostate cancer cell line)
Permeabilization
Yes - 0.1% Triton X-100
Specification
C42B Prostate cancer cell line
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 12 2020

Western blot abreview for Anti-JAK2 antibody [EPR108(2)]

Average
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (DU145 Prostate cancer cell line)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
DU145 Prostate cancer cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 04 2020

Western blot abreview for Anti-JAK2 antibody [EPR108(2)]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing (4–15%)
Sample
Mouse Tissue lysate - whole (Lung)
Specification
Lung
Blocking step
BSA as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jun 23 2014

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