Anti-JAM-C antibody [19 H36] (ab16889)

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Key features and details

  • Rat monoclonal [19 H36] to JAM-C
  • Suitable for: IP, Flow Cyt, IHC-Fr
  • Reacts with: Mouse, Human
  • Isotype: IgG2a

Overview

  • Product name

    Anti-JAM-C antibody [19 H36]
    See all JAM-C primary antibodies

  • Description

    Rat monoclonal [19 H36] to JAM-C

  • Host species

    Rat

  • Tested applications

    Suitable for: IP, Flow Cyt, IHC-Frmore details
    Unsuitable for: IHC-P or WB

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    This information is considered to be commercially sensitive.

  • Positive control

    • High endothelial venules of lymphoid organs, lymphoendothelial cells and endothelial cells of the kidney

  • General notes

    Previously labelled as Junctional Adhesion Molecule C.

Properties

Applications

Our Abpromise guarantee covers the use of ab16889 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 
IHC-Fr Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IHC-P or WB.

    • Target

    • Function

      Participates in cell-cell adhesion. It is a counterreceptor for ITGAM, mediating leukocyte-platelet interactions and is involved in the regulation of transepithelial migration of polymorphonuclear neutrophils (PMN). The soluble form is a mediator of angiogenesis.

    • Tissue specificity

      Highest expression in placenta, brain and kidney. Significant expression is detected on platelets. Expressed in intestinal mucosa cells. Expressed in the vascular endothelium. Found in serum (at protein level). Also detected in the synovial fluid of patients with rheumatoid arthritis, psoriatic arthritis or ostearthritis (at protein level).

    • Involvement in disease

      Defects in JAM3 are the cause of hemorrhagic destruction of the brain with subependymal calcification and cataracts (HDBSCC) [MIM:613730]. A syndrome characterized by congenital cataracts and severe brain abnormalities apparently resulting from hemorrhagic destruction of the brain tissue, including the cerebral white matter and basal ganglia. Patients manifest profound developmental delay, and other neurologic features included seizures, spasticity, and hyperreflexia. Brain imaging shows multifocal intraparenchymal hemorrhage with associated liquefaction and massive cystic degeneration, and calcification in the subependymal region and in brain tissue.

    • Sequence similarities

      Belongs to the immunoglobulin superfamily.
      Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.

    • Post-translational
      modifications

      Proteolytically cleaved from endothelial cells surface into a soluble form by ADAM10 and ADAM17; the release of soluble JAM3 is increased by proinflammatory factors.

    • Cellular localization

      Cell membrane. Cell junction > desmosome. Secreted > extracellular space. In epithelial cells, it is expressed at desmosomes but not at tight junctions. Localizes at the cell surface of endothelial cells; treatment of endothelial cells with vascular endothelial growth factor stimulates recruitement of JAM3 to cell-cell contacts.
    • Information by UniProt

    • Database links

      • Alternative names

        • FLJ14529 antibody
        • JAM 2 antibody
        • JAM 3 antibody
        • JAM C antibody
        • JAM-2 antibody
        • JAM-3 antibody
        • JAM-C antibody
        • JAM2 antibody
        • Jam3 antibody
        • JAM3_HUMAN antibody
        • JAMC antibody
        • Junctional adhesion molecule 3 antibody
        • Junctional adhesion molecule 3 precursor antibody
        • Junctional adhesion molecule C antibody
        see all

      References

      Publishing research using ab16889? Please let us know so that we can cite the reference in this datasheet.

      ab16889 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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      1-2 of 2 Abreviews or Q&A

      Question

      Please confirm which target these antibodies are detecting, as they have the same clone name [CRAM-19 H36]:  Junctional Adhesion Molecule C antibodies ab16906 ab16889 Junctional Adhesion Molecule 2 antibody ab22539 ab23643 There is some controversy over the naming of this protein family group. Please confirm.

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      Answer

      Thank you once again for your recent enquiries. I have investigated this case and have obtained the following information for you regarding out JAMC and JAM 2 antibodies. I can understand the confusion, and hope this will help. The SwissProt/Uniprot entry for JAM C states this as JAM 3 and JAM 2, which is where some of the confusion may be. The SwisProt/Uniprot entry link on the datasheet will be the correct one for the target of that antibody. I can confirm the following: ab16906 Junctional Adhesion Molecule C antibody [CRAM-18 F26]        This is detecting JAM 3, Junctional adhesion molecule C. The correct Swissprot/Uniprot ID is on the datasheet: http://www.uniprot.org/uniprot/Q9BX67 http://www.uniprot.org/uniprot/B3KWG9 ab22539 Junctional Adhesion Molecule 2 antibody [CRAM-18 F26]    This is the same clone, detecing JAM 3. junctional adhesion molecule C. http://www.uniprot.org/uniprot/Q9D8B7 (I have requested that the SwissProt/Uniprot ID link on our datasheet is corrected as I as there has been an error with this entry on the datasheet, my apologies) ab23643 Junctional Adhesion Molecule 2 antibody [CRAM-19 H36]     This is a different clone. It detects JAM 2, or JAM C. http://www.uniprot.org/uniprot/P57087 ab16889 Junctional Adhesion Molecule C antibody [19 H36]     Regrettably, I am sorry we had the incorrect clone number for this antibody. I have been informed this this is clone [19 H36]. This is detecting JAM C (JAM 3). http://www.uniprot.org/uniprot/B3KWG9 I hope this will be helpful. If you have any further questions, please do not hesitate to contact us.

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      Question

      We received an inquiry from the customer who purcahsed Jam-2 antibody, #ab16889 Lot 115504. Although he used it for staining of frozen sections, it did not work well. His conditions were as follows: 1) Type of sample: 4% PFA fixed, frozen sections of mouse testis 2) Blocking: for 1 hour at room temp in 0.5% milk PBST 3) 1st antibody: 1:10, for 72 minutes at 4 degrees in 0.5% milk TPBS 4) 2nd antibody: anti rat IgG-FITC from [a competitor] This 2nd antibody is working well using other primary antibodies. 5) Description of his problem: No signal 6) Any other 1st antibody(i.g. nectin2 and c-Kit) are working in these systems. He also didn't see any signals using JAM-2 antibody from another company. Would you please advise me how I should advise him in this case? Also, please let me know positive control you recommend. Best Regards,

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      Answer

      I'm sorry you have been experiencing a problem with ab16889. As positive control for staining JAM-2 on frozen sections murine tissue of high endothelial venules of lymphoid organs can be used. Or lymphoendothelial cells and the endothelial cells of the kidney. Because this antibody is cross-reactive with human it is possible to use human HUVEC monolayers as positive control to. I would recommend the following modifications to your protocol to improve the staining: -try fresh frozen sections postfixed with methanol (10min), -block with normal serum -incubate the primary antibody overnight in PBST -try with biotin-ABC-DAB detection rather than IF -make sure the amount of tritonx100 in the dilution and blocking buffer is adequate, typically 0.3% v/v in PBS. I hope these suggestions will help, please do not hesitate to contact us if you require further assistance,

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