Recombinant Anti-JAML antibody [EPR15289] (ab183714)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15289] to JAML
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-JAML antibody [EPR15289]
See all JAML primary antibodies -
Description
Rabbit monoclonal [EPR15289] to JAML -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal liver, Jurkat, Molt-4, 293, C6 and Raw264.7 cell lysates; Molt-4 cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15289 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183714 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 44 kDa).
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IP |
1/40 - 1/60.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 44 kDa). |
IP
1/40 - 1/60. |
Target
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Function
May function in transmigration of leukocytes through epithelial and endothelial tissues. Expressed at the plasma membrane of polymorphonuclear leukocytes, it mediates adhesive interactions with CXADR, a protein of the junctional complex of epithelial cells. Enhances myeloid leukemia cell adhesion to endothelial cells. -
Tissue specificity
Expression is restricted to the hematopoietic tissues with the exception of liver. Expressed in fetal liver, spleen and thymus. -
Sequence similarities
Belongs to the immunoglobulin superfamily.
Contains 2 Ig-like V-type (immunoglobulin-like) domains. -
Domain
The Ig-like V-type domain 2 mediates interaction with CXADR. -
Cellular localization
Cell junction. Cell membrane. Localized at the plasma membrane in areas of cell-cell contacts and at the cell surface of PMN. - Information by UniProt
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Database links
- Entrez Gene: 120425 Human
- Entrez Gene: 270152 Mouse
- Entrez Gene: 315610 Rat
- Omim: 609770 Human
- SwissProt: Q86YT9 Human
- SwissProt: Q80UL9 Mouse
- Unigene: 16291 Human
- Unigene: 190461 Mouse
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Alternative names
- adhesion molecule AMICA antibody
- Adhesion molecule interacting with CXADR antigen 1 antibody
- adhesion molecule interacts with CXADR antigen 1 antibody
see all
Images
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Western blot analysis of JAML in 293 cell lysate immunoprecipitated using ab183714 at 1/50 dilution (Lane 1). Lane 2 : TBS instead of 293 lysate.
Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution
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All lanes : Anti-JAML antibody [EPR15289] (ab183714) at 1/5000 dilution
Lane 1 : C6 (rat glioma cell line) whole cell lysate
Lane 2 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 44 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted? -
All lanes : Anti-JAML antibody [EPR15289] (ab183714) at 1/20000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : Jurkat cell lysate
Lane 3 : Molt-4 cell lysate
Lane 4 : 293 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 44 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (2)
ab183714 has been referenced in 2 publications.
- Wu Q et al. Junctional adhesion molecule-like protein promotes tumor progression via the Wnt/β-catenin signaling pathway in lung adenocarcinoma. J Transl Med 20:260 (2022). PubMed: 35672776
- Fang Y et al. Junctional Adhesion Molecule-Like Protein Promotes Tumor Progression and Metastasis via p38 Signaling Pathway in Gastric Cancer. Front Oncol 11:565676 (2021). PubMed: 33777731