Recombinant
RabMAb

Recombinant Anti-JAML antibody [EPR15289] (ab183714)

Overview

  • Product name

    Anti-JAML antibody [EPR15289]
    See all JAML primary antibodies
  • Description

    Rabbit monoclonal [EPR15289] to JAML
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human JAML aa 250 to the C-terminus. The exact sequence is proprietary.
    Database link: Q86YT9

  • Positive control

    • Human fetal liver, Jurkat, Molt-4, 293, C6 and Raw264.7 cell lysates; Molt-4 cells
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab183714 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 44 kDa).
IP 1/40 - 1/60.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function

      May function in transmigration of leukocytes through epithelial and endothelial tissues. Expressed at the plasma membrane of polymorphonuclear leukocytes, it mediates adhesive interactions with CXADR, a protein of the junctional complex of epithelial cells. Enhances myeloid leukemia cell adhesion to endothelial cells.
    • Tissue specificity

      Expression is restricted to the hematopoietic tissues with the exception of liver. Expressed in fetal liver, spleen and thymus.
    • Sequence similarities

      Belongs to the immunoglobulin superfamily.
      Contains 2 Ig-like V-type (immunoglobulin-like) domains.
    • Domain

      The Ig-like V-type domain 2 mediates interaction with CXADR.
    • Cellular localization

      Cell junction. Cell membrane. Localized at the plasma membrane in areas of cell-cell contacts and at the cell surface of PMN.
    • Information by UniProt
    • Database links

    • Alternative names

      • adhesion molecule AMICA antibody
      • Adhesion molecule interacting with CXADR antigen 1 antibody
      • adhesion molecule interacts with CXADR antigen 1 antibody
      • adhesion molecule that interacts with CXADR antigen 1 antibody
      • AMICA antibody
      • Amica1 antibody
      • CREA7-1 antibody
      • CREA7-4 antibody
      • Dendritic cell-specific protein CREA7-1 antibody
      • dendritic-cell specific protein CREA7-1 antibody
      • dendritic-cell specific protein CREA7-4 antibody
      • FLJ37080 antibody
      • Gm638 antibody
      • JAML1_HUMAN antibody
      • junctional adhesion molecule antibody
      • Junctional adhesion molecule-like antibody
      • Junctional adhesion molecule-like precursor antibody
      • MGC118814 antibody
      • MGC118815 antibody
      • UNQ722/PRO1387 antibody
      see all

    Images

    • Western blot analysis of JAML in 293 cell lysate immunoprecipitated using ab183714 at 1/50 dilution (Lane 1). Lane 2 : TBS instead of 293 lysate.

      Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution

    • All lanes : Anti-JAML antibody [EPR15289] (ab183714) at 1/5000 dilution

      Lane 1 : C6 cell lysate
      Lane 2 : Raw264.7 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

      Predicted band size: 44 kDa
      Observed band size: 60 kDa
      why is the actual band size different from the predicted?

    • All lanes : Anti-JAML antibody [EPR15289] (ab183714) at 1/20000 dilution

      Lane 1 : Human fetal liver lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : Molt-4 cell lysate
      Lane 4 : 293 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

      Predicted band size: 44 kDa
      Observed band size: 60 kDa why is the actual band size different from the predicted?

    References

    ab183714 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (bone marrow)
    Gel Running Conditions
    Non-reduced Denaturing
    Loading amount
    1e+006 cells
    Specification
    bone marrow
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

    Abcam user community

    Verified customer

    Submitted Nov 29 2018

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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