Recombinant
RabMAb

Recombinant Anti-JAML antibody [EPR15289] - BSA and Azide free (ab232632)

Overview

  • Product name

    Anti-JAML antibody [EPR15289] - BSA and Azide free
    See all JAML primary antibodies
  • Description

    Rabbit monoclonal [EPR15289] to JAML - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human JAML aa 250 to the C-terminus. The exact sequence is proprietary.
    Database link: Q86YT9

  • Positive control

    • WB: C6 and RAW 264.7 whole cell lysates.
  • General notes

    Ab232632 is the carrier-free version of ab183714. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab232632 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab232632 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 60 kDa (predicted molecular weight: 44 kDa).
IP Use at an assay dependent concentration.

Target

  • Function

    May function in transmigration of leukocytes through epithelial and endothelial tissues. Expressed at the plasma membrane of polymorphonuclear leukocytes, it mediates adhesive interactions with CXADR, a protein of the junctional complex of epithelial cells. Enhances myeloid leukemia cell adhesion to endothelial cells.
  • Tissue specificity

    Expression is restricted to the hematopoietic tissues with the exception of liver. Expressed in fetal liver, spleen and thymus.
  • Sequence similarities

    Belongs to the immunoglobulin superfamily.
    Contains 2 Ig-like V-type (immunoglobulin-like) domains.
  • Domain

    The Ig-like V-type domain 2 mediates interaction with CXADR.
  • Cellular localization

    Cell junction. Cell membrane. Localized at the plasma membrane in areas of cell-cell contacts and at the cell surface of PMN.
  • Information by UniProt
  • Database links

  • Alternative names

    • adhesion molecule AMICA antibody
    • Adhesion molecule interacting with CXADR antigen 1 antibody
    • adhesion molecule interacts with CXADR antigen 1 antibody
    • adhesion molecule that interacts with CXADR antigen 1 antibody
    • AMICA antibody
    • Amica1 antibody
    • CREA7-1 antibody
    • CREA7-4 antibody
    • Dendritic cell-specific protein CREA7-1 antibody
    • dendritic-cell specific protein CREA7-1 antibody
    • dendritic-cell specific protein CREA7-4 antibody
    • FLJ37080 antibody
    • Gm638 antibody
    • JAML1_HUMAN antibody
    • junctional adhesion molecule antibody
    • Junctional adhesion molecule-like antibody
    • Junctional adhesion molecule-like precursor antibody
    • MGC118814 antibody
    • MGC118815 antibody
    • UNQ722/PRO1387 antibody
    see all

Images

  • Western blot analysis of JAML in 293 cell lysate immunoprecipitated using ab183714 at 1/50 dilution (Lane 1). Lane 2 : TBS instead of 293 lysate.

    Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183714).

  • All lanes : Anti-JAML antibody [EPR15289] (ab183714) at 1/5000 dilution

    Lane 1 : C6 (rat glioma cell line) whole cell lysate
    Lane 2 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 44 kDa
    Observed band size: 60 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183714).

References

ab232632 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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