Janus Green cell normalization stain (ab111622)

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Overview

  • Product name
    Janus Green cell normalization stain
  • Tested applications
    Suitable for: In-Cell ELISAmore details
  • General notes
    Cells fixed to a solid surface (typically in microplate wells) can be stained by adding 1X Janus Green Stain. Incubate cells for 5 minutes at room temperature. Dye should be removed and washed 5 times in ultrapure water or until excess dye is removed. The last wash should be removed and add 0.1 mL 0.5 M HCl per well and incubate for 10 minutes. Shake the plate for 10 seconds, and measure the OD 595 nm using a standard microplate spectrophotometer.

Properties

  • Form
    Liquid
  • Storage instructions
    Store at +4°C.
  • Storage buffer
    Constituents: 0.02% Monobasic dihydrogen potassium phosphate, 0.216% Dibasic monohydrogen sodium phosphate, 0.02% Potassium chloride, 0.81% Sodium chloride, Water, 0.3% Janus Green B
  • Concentration information loading...
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab111622 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
In-Cell ELISA Use at an assay dependent dilution. 50 µL/ well fixed cells

References

ab111622 has not yet been referenced specifically in any publications.

Publishing research using ab111622? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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2 reviews or Q&A

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For this janus green assay, the protocol states to read the plate at 595nM. Can the absorbance of the 0.1 ml HCl solution be read in the same wells the cells were grown or do you recommend spinning down the plate, removing the solution to clean well pr...

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Yes, the OD 595 can be read directly on the plate where the cells were grown and the assay was performed. The HCL will disrupt the cells and the janus green (which stains the nuclei) will be released into the well. No need to spin or move the HCL to a ...

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Dear Sirs,
a customer of ours would like to know which is the right wavelenght to read the signal of Janus Green.

Thanks in advance
Kind regards

Thank you for your enquiry,

We recommend to measure the assays results at OD595 nm using a standard microplate spectrophotometer. We will be amending the datasheet to make this clearer.

Whole Cell Staining with Janus Green:
...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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