Overview

  • Product name
    Anti-Japanese encephalitis virus E glycoprotein antibody [JE1]
  • Description
    Mouse monoclonal [JE1] to Japanese encephalitis virus E glycoprotein
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, ELISA, Dot blot, ICC/IFmore details
  • Species reactivity
    Reacts with: Japanese encephalitis virus
  • Immunogen

    Japanese encephalitis virus infected supernatant

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.065% Sodium azide
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    JE1
  • Isotype
    IgG2a
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab41671 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. PubMed: 21124882
ELISA Use at an assay dependent dilution.
Dot blot Use at an assay dependent dilution.
ICC/IF 1/5 - 1/20.

Target

  • Relevance
    Japanese encephalitis virus E glycoprotein is one of three structural proteins encoded by the viral genome. It is thought to be involved in viral adhesion and entry into host cells, hemagglutination, cellular tropism, viral virulence, and the induction of protective immune responses. Japanese encephalitis (JE) is a Flaviviruses; these are small, enveloped RNA viruses that use arthropods such as mosquitoes for transmission to their vertebrate hosts. Flaviviruses consist of three structural proteins: the core nucleocapsid protein C, and the envelope glycoproteins M and E. Glycoprotein E is a class II viral fusion protein that mediates both receptor binding and fusion. Glycoprotein E is comprised of three domains: domain I (dimerisation domain) is an 8-stranded beta barrel, domain II (central domain) is an elongated domain composed of twelve beta strands and two alpha helices, and domain III (immunoglobulin-like domain) is an IgC-like module with ten beta strands. Domains I and II are intertwined. Domain II can be divided into two structural components, both of which comprise alpha-beta sandwich folds.
  • Cellular localization
    membrane
  • Database links
    • Alternative names
      • Japanese encephalitis E glycoprotein antibody
      • Japanese encephalitis envelope glycoprotein antibody
      • Japanese encephalitis virus envelope glycoprotein antibody
      • JEV E glycoprotein antibody
      • JEV envelope glycoprotein antibody
      • Major envelope protein E antibody
      see all

    References

    This product has been referenced in:
    • Li XF  et al. Visualization of a neurotropic flavivirus infection in mouse reveals unique viscerotropism controlled by host type I interferon signaling. Theranostics 7:912-925 (2017). Read more (PubMed: 28382163) »
    • Nain M  et al. GRP78 Is an Important Host Factor for Japanese Encephalitis Virus Entry and Replication in Mammalian Cells. J Virol 91:N/A (2017). Read more (PubMed: 28053106) »
    See all 5 Publications for this product

    Customer reviews and Q&As

    1-6 of 6 Abreviews or Q&A

    Answer

    Thank you very much for your inquiry and please apologize for the delay in the answer.

    1.) The range of the concentration has not been determined, however the laboratory confirmed thatit is normally around 5-20ug/ml.

    2.) The QC for thismonoclonal antibody is as per datasheet for presence of specific reactivity. If your customer needs to know moreabout amount, the laboratory recommneds to titrate the reagent starting at 1:100 dilution in a simple mouse antibody sandwich assay. Briefly, the steps would be to

    coat wells with anti-mouse Ig, to block with BSA, to titrate the monoclonal antibody supernatantfor 1hr RT, to add the secondary antibody anti-mouse Ig-HRP 1hr RT then to add the substrate. I hope this helps. Please let me know what kind of documentation you were looking for. If zou canprovide me with the batch number the customer has, I can provide you also with a certificate of compliance.



    3.) We do not have more information in this regards. I still can however confirm that I would be very happy to provide a testing discount (https://www.abcam.com/test-vial) to the customer to test the ab26950 with either the ab81193 or the ab41671, I will be happy to provide it.



    I hope this information is helpful. Please let me know if you have any questions.

    Read More

    Answer

    Thank you for contacting us.

    The product ab41651 was used in IHC-FoFr (Paraffin perfused, frozen sections). We haven't tested this product in Immunohistochemistry in our own lab so I am sorry we will not be able to share the results with you. You have to try these product yourselves; we can only provide the testing discount if you are interested, the protocol conditions must also be optimized by the end user.

    Please let me know if you want to send you a 1005 Abreview discount code. Please also note that Abcam do not provide free of charge samples however we are happy to collaborate with you for the testing discount.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

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    Answer

    Thank you very much for your interest in ab41671.

    To our knowledge, ab41671 has not been tested in IHC. Therefore, I can offer a discount off a future purchase if you buy ab41671 now, test it inIHC and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free (PRIMARY ANTIBODY/PROTEIN) OR VALUE OFF OF NEXT ORDER.

    If you are interested in this offer, please follow these steps:

    1. Reply to this e-mail to let me know that you would like to proceed and test ab41671 in (IHC). I will then send a discount code. This code must be issued before purchasing ab41671 so please wait for my reply before ordering.

    2. Purchase ab41671 either by phone, fax, or online (www.abcam.com).

    3. Test it in IHC.

    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any [PRIMARY ANTIBODY /PROTEIN /PRODUCT] ordered and the discount code is valid for 4 months after issue.
    Please remember that submission of the Abreview is sufficient for the discount code to become active.

    We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab41671 turns out to be unsuitable for IHC, you will still receive the discount on your next purchase after your Abreview has been submitted.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: https://www.abcam.com/collaborationdiscount.

    PS: We have never tested this antibody with Indian strains so we will not be able to confirm its suitability. If you are tested in testing discount could you specify if you want testing discount for IHC-P, IHC-FR or IHC-FoFr.

    Read More

    Answer

    Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results.

    The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful.

    The protocol seems perfectly fine to me, but I would just want to confirm the transfection of JEV envelope has been done successfully, and checked by other techniques.

    I would also appreciate if you could please send me the order details, and if the antibody is still under the guarantee period I would be happy to send a replacement or reimbursement for it.

    I look forward for receiving your reply.

    Read More

    Answer

    Thank you for contacting us. Please find attached the COC documents you requested. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Answer

    Thank you for contacting us. We have not tested these antibodies together in sELISA so we unfortunately can't confirm their use. Theoretically it seems the antibodies will work however we cannot be sure until the tested data. As the customer has expressed interest in using these antibodies in sELISA I would be happy to provide Abreview discount codes if these antibodies tested together in sELISA. Let me know if you would like to receive discount codes. I hope this will info will be helpful. Should you have any other question please do not hesitate to ask.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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