Anti-Japanese encephalitis virus E glycoprotein antibody [JE1] (ab41671)
Key features and details
- Mouse monoclonal [JE1] to Japanese encephalitis virus E glycoprotein
- Suitable for: WB, ELISA, Dot blot, ICC/IF
- Reacts with: Japanese encephalitis virus
- Isotype: IgG2a
Overview
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Product name
Anti-Japanese encephalitis virus E glycoprotein antibody [JE1] -
Description
Mouse monoclonal [JE1] to Japanese encephalitis virus E glycoprotein -
Host species
Mouse -
Tested applications
Suitable for: WB, ELISA, Dot blot, ICC/IFmore details -
Species reactivity
Reacts with: Japanese encephalitis virus -
Immunogen
Tissue, cells or virus corresponding to Japanese encephalitis virus Japanese encephalitis virus E glycoprotein.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Proclin 150
Constituents: 10% BSA, 89.9% RPMI 1640 -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
JE1 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab41671 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. PubMed: 21124882
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ELISA |
Use at an assay dependent concentration.
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Dot blot |
Use at an assay dependent concentration.
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ICC/IF |
1/5 - 1/20.
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Notes |
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WB
Use at an assay dependent concentration. PubMed: 21124882 |
ELISA
Use at an assay dependent concentration. |
Dot blot
Use at an assay dependent concentration. |
ICC/IF
1/5 - 1/20. |
Target
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Relevance
Japanese encephalitis virus E glycoprotein is one of three structural proteins encoded by the viral genome. It is thought to be involved in viral adhesion and entry into host cells, hemagglutination, cellular tropism, viral virulence, and the induction of protective immune responses. Japanese encephalitis (JE) is a Flaviviruses; these are small, enveloped RNA viruses that use arthropods such as mosquitoes for transmission to their vertebrate hosts. Flaviviruses consist of three structural proteins: the core nucleocapsid protein C, and the envelope glycoproteins M and E. Glycoprotein E is a class II viral fusion protein that mediates both receptor binding and fusion. Glycoprotein E is comprised of three domains: domain I (dimerisation domain) is an 8-stranded beta barrel, domain II (central domain) is an elongated domain composed of twelve beta strands and two alpha helices, and domain III (immunoglobulin-like domain) is an IgC-like module with ten beta strands. Domains I and II are intertwined. Domain II can be divided into two structural components, both of which comprise alpha-beta sandwich folds. -
Cellular localization
membrane -
Database links
- SwissProt: O09754 Japanese encephalitis virus
- SwissProt: P27395 Japanese encephalitis virus
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Alternative names
- Japanese encephalitis E glycoprotein antibody
- Japanese encephalitis envelope glycoprotein antibody
- Japanese encephalitis virus envelope glycoprotein antibody
see all
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab41671 has been referenced in 8 publications.
- Soni N et al. Bone marrow-derived extracellular vesicles modulate the abundance of infiltrating immune cells in the brain and exert an antiviral effect against the Japanese encephalitis virus. FASEB Bioadv 4:798-815 (2022). PubMed: 36479206
- Tripathi A et al. Development and characterization of an animal model of Japanese encephalitis virus infection in adolescent C57BL/6 mouse. Dis Model Mech 14:N/A (2021). PubMed: 34447981
- Li XF et al. Visualization of a neurotropic flavivirus infection in mouse reveals unique viscerotropism controlled by host type I interferon signaling. Theranostics 7:912-925 (2017). PubMed: 28382163
- Nain M et al. GRP78 Is an Important Host Factor for Japanese Encephalitis Virus Entry and Replication in Mammalian Cells. J Virol 91:N/A (2017). PubMed: 28053106
- Li M et al. Japanese encephalitis virus counteracts BST2 restriction via its envelope protein E. Virology 510:67-75 (2017). PubMed: 28710958
- Sharma M et al. Japanese encephalitis virus replication is negatively regulated by autophagy and occurs on LC3-I- and EDEM1-containing membranes. Autophagy 10:1637-51 (2014). PubMed: 25046112
- Das S et al. Critical role of lipid rafts in virus entry and activation of phosphoinositide 3' kinase/Akt signaling during early stages of Japanese encephalitis virus infection in neural stem/progenitor cells. J Neurochem 115:537-49 (2010). ICC/IF ; Mouse . PubMed: 20722967
- Nazmi A et al. Antiviral and neuroprotective role of octaguanidinium dendrimer-conjugated morpholino oligomers in Japanese encephalitis. PLoS Negl Trop Dis 4:e892 (2010). WB ; Japanese encephalitis virus . PubMed: 21124882