Anti-Japanese encephalitis virus NS1 glycoprotein antibody [JN1] (ab41651)
Key features and details
- Mouse monoclonal [JN1] to Japanese encephalitis virus NS1 glycoprotein
- Suitable for: IHC-FoFr, Flow Cyt, WB, ICC/IF, ELISA
- Reacts with: Japanese encephalitis virus
- Isotype: IgG3
Overview
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Product name
Anti-Japanese encephalitis virus NS1 glycoprotein antibody [JN1]
See all Japanese encephalitis virus NS1 glycoprotein primary antibodies -
Description
Mouse monoclonal [JN1] to Japanese encephalitis virus NS1 glycoprotein -
Host species
Mouse -
Tested applications
Suitable for: IHC-FoFr, Flow Cyt, WB, ICC/IF, ELISAmore details -
Species reactivity
Reacts with: Japanese encephalitis virus -
Immunogen
Full length native protein purified from Japanese encephalitis virus (Nakayama) supernatant
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Proclin 150
Constituents: 10% BSA, 89.9% RPMI 1640 -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
JN1 -
Isotype
IgG3 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab41651 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-FoFr |
Use at an assay dependent concentration. PubMed: 19635909
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Flow Cyt |
Use at an assay dependent concentration. PubMed: 20581148
ab18392 - Mouse monoclonal IgG3, is suitable for use as an isotype control with this antibody. |
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WB |
1/50 - 1/100. Use under non reducing condition. Predicted molecular weight: 46 kDa.
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ICC/IF |
1/5 - 1/20.
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ELISA |
Use at an assay dependent concentration.
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Notes |
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IHC-FoFr
Use at an assay dependent concentration. PubMed: 19635909 |
Flow Cyt
Use at an assay dependent concentration. PubMed: 20581148 ab18392 - Mouse monoclonal IgG3, is suitable for use as an isotype control with this antibody. |
WB
1/50 - 1/100. Use under non reducing condition. Predicted molecular weight: 46 kDa. |
ICC/IF
1/5 - 1/20. |
ELISA
Use at an assay dependent concentration. |
Target
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Relevance
The Japanese encephalitis viral genome encodes 7 non-structural proteins NS1-NS5. NS1 contains N-linked carbohydrate chains at positions 130 and 207. It is not incorporated into the virion but exists in the host cell, on the cell surface and can also be extracellular. -
Database links
- SwissProt: P27395 Japanese encephalitis virus
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Alternative names
- Japanese encephalitis non-structural protein 1 antibody
- Japanese encephalitis NS1 antibody
- Japanese encephalitis virus non-structural protein 1 antibody
Images
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Japanese encephalitis virus (Nakayama) infected PS clone D cells stained with ab41651 (green).
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All lanes : Anti-Japanese encephalitis virus NS1 glycoprotein antibody [JN1] (ab41651)
Lane 1 : Japanese encephalitis virus infected C6/36 cell lysate (unheated)
Lane 2 : Japanese encephalitis virus infected C6/36 cell lysate (boiled)
Predicted band size: 46 kDa
Observed band size: 46,92 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa (possible cleavage fragment), 50 kDa (possible cleavage fragment)
This antibody recognsies 2 forms of NS1 - NS1 and NS1' (46 and 53 kDa respectively). NS1' is thought to be formed when NS1 is cleaved from NS2A at an alternative site. Both NS1 and NS1' exist as dimers in untreated samples but are dissociated into monomers when samples are boiled.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab41651 has been referenced in 9 publications.
- Sarkar R et al. Japanese encephalitis virus capsid protein interacts with non-lipidated MAP1LC3 on replication membranes and lipid droplets. J Gen Virol 102:N/A (2021). PubMed: 33095129
- Li XF et al. Development of a chimeric Zika vaccine using a licensed live-attenuated flavivirus vaccine as backbone. Nat Commun 9:673 (2018). PubMed: 29445153
- Sharma N et al. Japanese Encephalitis Virus exploits the microRNA-432 to regulate the expression of Suppressor of Cytokine Signaling (SOCS) 5. Sci Rep 6:27685 (2016). WB ; Japanese encephalitis virus . PubMed: 27282499
- Sharma N et al. miR-146a suppresses cellular immune response during Japanese encephalitis virus JaOArS982 strain infection in human microglial cells. J Neuroinflammation 12:30 (2015). WB ; Japanese encephalitis virus . PubMed: 25889446
- Sharma M et al. Japanese encephalitis virus replication is negatively regulated by autophagy and occurs on LC3-I- and EDEM1-containing membranes. Autophagy 10:1637-51 (2014). PubMed: 25046112
- Li XF et al. A chimeric dengue virus vaccine using Japanese encephalitis virus vaccine strain SA14-14-2 as backbone is immunogenic and protective against either parental virus in mice and nonhuman primates. J Virol 87:13694-705 (2013). PubMed: 24109223
- Zhang T et al. Anti- Japanese-Encephalitis-Viral Effects of Kaempferol and Daidzin and Their RNA-Binding Characteristics. PLoS One 7:e30259 (2012). WB ; Japanese encephalitis virus . PubMed: 22276167
- Aleyas AG et al. Multifront Assault on Antigen Presentation by Japanese Encephalitis Virus Subverts CD8+ T Cell Responses. J Immunol : (2010). Flow Cyt . PubMed: 20581148
- Aleyas AG et al. Functional modulation of dendritic cells and macrophages by Japanese encephalitis virus through MyD88 adaptor molecule-dependent and -independent pathways. J Immunol 183:2462-74 (2009). IHC-FoFr . PubMed: 19635909