Key features and details
- Mouse monoclonal [JN1] to Japanese encephalitis virus NS1 glycoprotein
- Suitable for: WB, ICC/IF, ELISA
- Reacts with: Japanese encephalitis virus
- Isotype: IgG3
Product nameAnti-Japanese encephalitis virus NS1 glycoprotein antibody [JN1]
See all Japanese encephalitis virus NS1 glycoprotein primary antibodies
DescriptionMouse monoclonal [JN1] to Japanese encephalitis virus NS1 glycoprotein
Tested applicationsSuitable for: WB, ICC/IF, ELISAmore details
Species reactivityReacts with: Japanese encephalitis virus
Full length native protein purified from Japanese encephalitis virus (Nakayama) supernatant
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.065% Sodium azide
Concentration information loading...
PurityTissue culture supernatant
Light chain typekappa
Our Abpromise guarantee covers the use of ab41651 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/50 - 1/100. Use under non reducing condition. Predicted molecular weight: 46 kDa.|
|ICC/IF||1/5 - 1/20.|
|ELISA||Use at an assay dependent concentration.|
RelevanceThe Japanese encephalitis viral genome encodes 7 non-structural proteins NS1-NS5. NS1 contains N-linked carbohydrate chains at positions 130 and 207. It is not incorporated into the virion but exists in the host cell, on the cell surface and can also be extracellular.
- SwissProt: P27395 Japanese encephalitis virus
- Japanese encephalitis non-structural protein 1 antibody
- Japanese encephalitis NS1 antibody
- Japanese encephalitis virus non-structural protein 1 antibody
Japanese encephalitis virus (Nakayama) infected PS clone D cells stained with ab41651 (green).
All lanes : Anti-Japanese encephalitis virus NS1 glycoprotein antibody [JN1] (ab41651)
Lane 1 : Japanese encephalitis virus infected C6/36 cell lysate (unheated)
Lane 2 : Japanese encephalitis virus infected C6/36 cell lysate (boiled)
Predicted band size: 46 kDa
Observed band size: 46,92 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa (possible cleavage fragment), 50 kDa (possible cleavage fragment)
This antibody recognsies 2 forms of NS1 - NS1 and NS1' (46 and 53 kDa respectively). NS1' is thought to be formed when NS1 is cleaved from NS2A at an alternative site. Both NS1 and NS1' exist as dimers in untreated samples but are dissociated into monomers when samples are boiled.
ab41651 has been referenced in 8 publications.
- Li XF et al. Development of a chimeric Zika vaccine using a licensed live-attenuated flavivirus vaccine as backbone. Nat Commun 9:673 (2018). PubMed: 29445153
- Sharma N et al. Japanese Encephalitis Virus exploits the microRNA-432 to regulate the expression of Suppressor of Cytokine Signaling (SOCS) 5. Sci Rep 6:27685 (2016). WB ; Japanese encephalitis virus . PubMed: 27282499
- Sharma N et al. miR-146a suppresses cellular immune response during Japanese encephalitis virus JaOArS982 strain infection in human microglial cells. J Neuroinflammation 12:30 (2015). WB ; Japanese encephalitis virus . PubMed: 25889446
- Sharma M et al. Japanese encephalitis virus replication is negatively regulated by autophagy and occurs on LC3-I- and EDEM1-containing membranes. Autophagy 10:1637-51 (2014). PubMed: 25046112
- Li XF et al. A chimeric dengue virus vaccine using Japanese encephalitis virus vaccine strain SA14-14-2 as backbone is immunogenic and protective against either parental virus in mice and nonhuman primates. J Virol 87:13694-705 (2013). PubMed: 24109223
- Zhang T et al. Anti- Japanese-Encephalitis-Viral Effects of Kaempferol and Daidzin and Their RNA-Binding Characteristics. PLoS One 7:e30259 (2012). WB ; Japanese encephalitis virus . PubMed: 22276167
- Aleyas AG et al. Multifront Assault on Antigen Presentation by Japanese Encephalitis Virus Subverts CD8+ T Cell Responses. J Immunol : (2010). Flow Cyt . PubMed: 20581148
- Aleyas AG et al. Functional modulation of dendritic cells and macrophages by Japanese encephalitis virus through MyD88 adaptor molecule-dependent and -independent pathways. J Immunol 183:2462-74 (2009). IHC-FoFr . PubMed: 19635909