Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 0.88% Sodium chloride, 99% Tris glycine
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab178561 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 2 µg/ml. Predicted molecular weight: 138 kDa.|
|ChIP||Use at an assay dependent concentration.|
|ICC/IF||1/50 - 1/200.|
FunctionRegulator of histone methyltransferase complexes that plays an essential role in embryonic development, including heart and liver development, neural tube fusion process and hematopoiesis. Acts by modulating histone methyltransferase activity and promoting the recruitment of histone methyltransferase complexes to their target genes. Binds DNA and mediates the recruitment of the PRC2 complex to target genes in embryonic stem cells. Does not have histone demethylase activity but regulates activity of various histone methyltransferase complexes. In embryonic stem cells, it associates with the PRC2 complex and inhibits trimethylation of 'Lys-27' of histone H3 (H3K27me3) by the PRC2 complex, thereby playing a key role in differentiation of embryonic stem cells and normal development. In cardiac cells, it is required to repress expression of cyclin-D1 (CCND1) by activating methylation of 'Lys-9' of histone H3 (H3K9me) by the GLP1/EHMT1 and G9a/EHMT2 histone methyltransferases. Also acts as a transcriptional repressor of ANF via its interaction with GATA4 and NKX2-5. Participates in the negative regulation of cell proliferation signaling.
Tissue specificityDuring embryogenesis, predominantly expressed in neurons and particularly in dorsal root ganglion cells.
Sequence similaritiesContains 1 ARID domain.
Contains 1 JmjC domain.
Contains 1 JmjN domain.
DomainThe ARID domain is required to target the PRC2 complex to its target genes.
The GSGFP motif is required for the interaction with SUZ12.
Cellular localizationNucleus. Colocalizes with the PRC2 complex on chromatin.
- Information by UniProt
- JARD2 antibody
- JARD2_HUMAN antibody
- JARID2 antibody
This product has been referenced in:
- Dahle Ø & Kuehn MR Inhibiting Smad2/3 signaling in pluripotent mouse embryonic stem cells enhances endoderm formation by increasing transcriptional priming of lineage-specifying target genes. Dev Dyn 245:807-15 (2016). Read more (PubMed: 27012147) »