Recombinant Anti-Jarid2 antibody [EPR6357(2)] - ChIP Grade (ab192252)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6357(2)] to Jarid2 - ChIP Grade
- Suitable for: WB, ICC/IF, ChIP
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Jarid2 antibody [EPR6357(2)] - ChIP Grade
See all Jarid2 primary antibodies -
Description
Rabbit monoclonal [EPR6357(2)] to Jarid2 - ChIP Grade -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, ChIPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- NCCIT, SH-SY5Y, 293 and U87-MG cell lysates; SH-SY5Y cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6357(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab192252 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
1/1000 - 1/10000. Detects a band of approximately 139 kDa (predicted molecular weight: 139 kDa).
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ICC/IF |
1/100.
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ChIP |
Use at an assay dependent concentration.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 139 kDa (predicted molecular weight: 139 kDa). |
ICC/IF
1/100. |
ChIP
Use at an assay dependent concentration. |
Target
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Function
Regulator of histone methyltransferase complexes that plays an essential role in embryonic development, including heart and liver development, neural tube fusion process and hematopoiesis. Acts by modulating histone methyltransferase activity and promoting the recruitment of histone methyltransferase complexes to their target genes. Binds DNA and mediates the recruitment of the PRC2 complex to target genes in embryonic stem cells. Does not have histone demethylase activity but regulates activity of various histone methyltransferase complexes. In embryonic stem cells, it associates with the PRC2 complex and inhibits trimethylation of 'Lys-27' of histone H3 (H3K27me3) by the PRC2 complex, thereby playing a key role in differentiation of embryonic stem cells and normal development. In cardiac cells, it is required to repress expression of cyclin-D1 (CCND1) by activating methylation of 'Lys-9' of histone H3 (H3K9me) by the GLP1/EHMT1 and G9a/EHMT2 histone methyltransferases. Also acts as a transcriptional repressor of ANF via its interaction with GATA4 and NKX2-5. Participates in the negative regulation of cell proliferation signaling. -
Tissue specificity
During embryogenesis, predominantly expressed in neurons and particularly in dorsal root ganglion cells. -
Sequence similarities
Contains 1 ARID domain.
Contains 1 JmjC domain.
Contains 1 JmjN domain. -
Domain
The ARID domain is required to target the PRC2 complex to its target genes.
The GSGFP motif is required for the interaction with SUZ12. -
Cellular localization
Nucleus. Colocalizes with the PRC2 complex on chromatin. - Information by UniProt
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Database links
- Entrez Gene: 3720 Human
- Entrez Gene: 16468 Mouse
- Entrez Gene: 681740 Rat
- Omim: 601594 Human
- SwissProt: Q92833 Human
- SwissProt: Q62315 Mouse
- Unigene: 269059 Human
- Unigene: 25059 Mouse
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Alternative names
- JARD2 antibody
- JARD2_HUMAN antibody
- JARID2 antibody
see all
Images
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Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: JARID2 (KO) knockout HAP1 whole cell lysate (20 µg)
Lane 3: SH-SY5Y whole cell lysate (20 µg)
Lane 4: U87MG whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab192252 observed at 138 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab192252 was shown to specifically recognize JARID2 in wild-type HAP1 cells along with additional cross reactive bands. No band was observed when JARID2 knockout samples were examined. Wild-type and JARID2 knockout samples were subjected to SDS-PAGE. Ab192252 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% triton X-100 permeabilized SH-SY5Y cells labeling Jarid2 with ab192252 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody (ab150078) at 1/500 dilution. Nuclear counter stain Dapi (blue).
The two negative controls are ab192252 at 1/100 dilution followed by Goat anti mouse IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution.
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Chromatin was prepared from HeLa cells according to the Abcam Dual X-ChIP protocol*. Cells were fixed with EGS for 30 minutes, then formaldehyde for 10 minutes.
The ChIP was performed with 25 µg of chromatin, 5 µg of ab192252 (red), and 20 µl of Protein A/G sepharose beads. 5 µg of rabbit normal IgG was added to the beads control (gray). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).
Primers and probes are located in the first kb of the transcribed region.
*http://www.abcam.com/resources?keywords=X%20ChIP%20protocol -
All lanes : Anti-Jarid2 antibody [EPR6357(2)] - ChIP Grade (ab192252) at 1/10000 dilution
Lane 1 : NCCIT cell lysate
Lane 2 : SH-SY5Y cell lysate
Lane 3 : 293 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 139 kDa
Observed band size: 139 kDaBlocking/Dilution buffer: 5% NFDM /TBST.
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Anti-Jarid2 antibody [EPR6357(2)] - ChIP Grade (ab192252) at 1/1000 dilution + U87-MG cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 139 kDa
Observed band size: 139 kDaBlocking/Dilution buffer: 5% NFDM /TBST.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (4)
ab192252 has been referenced in 4 publications.
- Liu Y et al. LINC00649 Facilitates the Cellular Process of Bladder Cancer Cells via Signaling Axis miR-16-5p/JARID2. Urol Int 106:304-312 (2022). PubMed: 33789312
- Kuang W et al. The function and mechanism of the JARID2/CCND1 axis in modulating glioma cell growth and sensitivity to temozolomide (TMZ). Cancer Biol Ther 22:392-403 (2021). PubMed: 34251962
- Liu Y et al. EZH2 regulates sFRP4 expression without affecting the methylation of sFRP4 promoter DNA in colorectal cancer cell lines. Exp Ther Med 20:33 (2020). PubMed: 32952624
- Nguyen K et al. Multiple Histone Lysine Methyltransferases Are Required for the Establishment and Maintenance of HIV-1 Latency. MBio 8:N/A (2017). WB, ChIP ; Human . PubMed: 28246360