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Regarding ab112134, what is the way to measure the blank? should it be growth medium only? Could you please explain how to make sense of the measurements? How do you go from fluorescence values to calculating apoptotic cells percentage? Thank you for your kind help.
Asked on Dec 27 2012
Thank you for contacting us about ab112134. Here is some further information: - The blank contains no cells and is growth media only. This gives the background and should be subtracted from all readings. - The control sample should have cells with no treatment. - ex/em = 490/520 (for green) and ex/em = 540/590 (for red) - The data is presented as a ratio of apoptotic (green) / normal cells (red). Therefore divide the 520 emission reading by the 590 emission reading. - In the graph, the ratio for control cells is set as 100%, and other ratio readings are shown relative to that, to show the proportion increase/decrease in apoptosis. I hope this information is helpful. Please do not hesitate to contact me should you have further questions.
Answered on Dec 27 2012