For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
If you continue without changing your cookie settings, we'll assume you’re happy with this.
I am currently using your JC-10 assay kit (ab112134). My colleague recommended the Mito-ID membrane potential cytotoxicity kit (please see product sheet attached). Enzo compares its sensitivity with JC-1, from which JC-10 has been developed as far as I understood. Do you have information how the sensitivity of the JC-10 assay kit compares to the Mito-ID assay kit?
Also, do you have any data on the JC-10 assay for primary mouse neurons? Performing the assay I get quite a big standard deviation, which makes it difficult to identify subtle differences between samples.
Asked on Dec 23 2014
Thank you for your enquiry.
JC-1, a lipophiliccationic dye, is widely used to detect mitochondrial de-polarization. However it has poor water solubility which means it precipitates in aqueous buffer even at 1 μM concentration. Compared to JC-1, JC-10 has much better water solubility. As in the case of JC-1, JC-10 is capable of entering selectively into mitochondria, and changes reversibly its color from green to orange as membrane potentials increase.
From assays performed on a fluorescence plate reader and fluorescence microscope, JC-10 outperforms JC-1 in several different cell types (e.g., primary rat hepatocytes, CHO-K1, HeLa, Jurkatand HepG2 cell lines). In most of the cell lines JC-10 has superior signal to background ratios. JC 10 also has smaller assay deviations due to its enhanced water solubility and higher sensitivity.
I am sorry we have no data from primary mouse neurons specifically in this case.
If you have some concerns regarding the results, please do not hesitate to contact me again with further details. The kit is covered by our 12 month guarantee and I will be pleased to provide further support. I would appreciate if it is possible to send:
1. Details of sample preparation.
2. The protocol used, and instrument used for reading.
3. A copy of the data.
I hope this will be helpful. I look forward to hearing from you with the requested information.
Sam WasherAbcam Scientific Support
Answered on Dec 23 2014