• Product name

  • Description

    Rabbit polyclonal to JDP2
  • Host species

  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: RatDoes not react with: Mouse
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Human JDP2.

    Read Abcam's proprietary immunogen policy (Peptide available as ab41625.)

  • Positive control

    • ab40916 gave a positive signal in WB on the following lysates: HeLa whole cell, HeLa nuclear, Jurkat whole cell, Jurkat nuclear, HepG2 nuclear, Du145 whole cell and LnCAP nuclear. ICC/IF: HepG2 cells. IHC-P: human cervix.



Our Abpromise guarantee covers the use of ab40916 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).
ICC/IF Use a concentration of 1 µg/ml.


  • Function

    Component of the AP-1 transcription factor that represses transactivation mediated by the Jun family of proteins. Involved in a variety of transcriptional responses associated with AP-1 such as UV-induced apoptosis, cell differentiation, tumorigenesis and antitumogeneris. Can also function as a repressor by recruiting histone deacetylase 3/HDAC3 to the promoter region of JUN. May control transcription via direct regulation of the modification of histones and the assembly of chromatin.
  • Sequence similarities

    Belongs to the bZIP family. ATF subfamily.
    Contains 1 bZIP domain.
  • Post-translational

    Phosphorylation of Thr-148 by MAPK8 in response to different stress conditions such as, UV irradiation, oxidatives stress and anisomycin treatments.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • Jdp2 antibody
    • JDP2_HUMAN antibody
    • Jun dimerization protein 2 antibody
    • JUNDM2 antibody
    • Progesterone receptor co-activator antibody
    see all


  • All lanes : Anti-JDP2 antibody (ab40916) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
    Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 4 : Jurkat nuclear extract lysate (ab14844)
    Lane 5 : HepG2 nuclear extract lysate (ab14660)
    Lane 6 : Du145 (Human prostate carcinoma cell line) Whole Cell Lysate
    Lane 7 : LNCaP (Human prostate adenocarcinoma cell line) Nuclear lysate

    Lysates/proteins at 10 µg per lane.

    All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 19 kDa
    Observed band size: 19 kDa

  • ICC/IF image of ab40916 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab40916, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab40916 staining in human cervix formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab40916, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.


This product has been referenced in:

  • Mansour MR  et al. JDP2: An oncogenic bZIP transcription factor in T cell acute lymphoblastic leukemia. J Exp Med 215:1929-1945 (2018). Read more (PubMed: 29941549) »
  • Tanigawa S  et al. Jun dimerization protein 2 is a critical component of the Nrf2/MafK complex regulating the response to ROS homeostasis. Cell Death Dis 4:e921 (2013). WB ; Human . Read more (PubMed: 24232097) »
See all 3 Publications for this product

Customer reviews and Q&As

This product is known to not work in this application or species.
Western blot
Mouse Cell lysate - whole cell (CD4+ T cells)
Loading amount
1e+006 cells
CD4+ T cells
Gel Running Conditions
Non-reduced Denaturing (4 - 12 % gradient)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

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Submitted Jun 26 2008

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