JEG-3 nuclear extract lysate (ab14841)

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Overview

  • Product name

    JEG-3 nuclear extract lysate

  • General notes

    We recommend aliquoting the extracts into single use fractions and then storing them.

  • Tested applications

    Suitable for: EMSA, WBmore details

Properties

  • Mycoplasma free

    Yes

  • Form

    Liquid

  • Storage instructions

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7.90
    Constituents: 20% Glycerol, 0.48% HEPES, 0.008% DTT, 0.01% Magnesium chloride, 0.009% PMSF, 0.75% Potassium chloride
  • Concentration information loading...

  • Research areas

  • Background

    The human placenta is a highly invasive tumor-like structure in which a subpopulation of placental trophoblast cells known as the "extravillous trophoblast" (EVT) invades the uterine decidua and its vasculature to establish adequate fetal-maternal exchange of molecules. In vitro-propagated short-lived EVT cell lines such as JEG-3 cells are used to study molecular mechanisms responsible for this invasiveness, which are identical to those of cancer cells; however, unlike cancer cells, their proliferation, migration, and invasiveness in situ are stringently controlled by decidua-derived transforming growth factor (TGF)-beta. These epithelial-trophoblast-like cells are therefore used in identifying genetic changes underlying epithelial tumor progression as well as gonadotrophin secretion studies.

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab14841 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
EMSA
Use at an assay dependent dilution.
WB
Use at an assay dependent dilution.
Notes
EMSA
Use at an assay dependent dilution.
WB
Use at an assay dependent dilution.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab14841? Please let us know so that we can cite the reference in this datasheet.

ab14841 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Question

For the product ab14841 (JEG-3 cell line), do you know if it is HLA-G positive?

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Answer

Thank you for your enquiry. JEG-3 cells are known to express the HLA-G protein. Please note though that ab14841 is a nuclear lysate, so HLA-G protein levels are expected to be low, if at all. There are a lot of publications on JEG-3 expressing HLA-G. For example: http://www.ncbi.nlm.nih.gov/pubmed/9831130 and an update: http://www.ncbi.nlm.nih.gov/pubmed/19508383 I hope this information helps. Please do not hesitate to contact us if you need any more advice or information.

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