JEG-3 nuclear extract lysate (ab14841)
Overview
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Product name
JEG-3 nuclear extract lysate -
General notes
We recommend aliquoting the extracts into single use fractions and then storing them. -
Tested applications
Suitable for: EMSA, WBmore details
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.90
Constituents: 20% Glycerol, 0.48% HEPES, 0.008% DTT, 0.01% Magnesium chloride, 0.009% PMSF, 0.75% Potassium chloride -
Concentration information loading...
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Research areas
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Background
The human placenta is a highly invasive tumor-like structure in which a subpopulation of placental trophoblast cells known as the "extravillous trophoblast" (EVT) invades the uterine decidua and its vasculature to establish adequate fetal-maternal exchange of molecules. In vitro-propagated short-lived EVT cell lines such as JEG-3 cells are used to study molecular mechanisms responsible for this invasiveness, which are identical to those of cancer cells; however, unlike cancer cells, their proliferation, migration, and invasiveness in situ are stringently controlled by decidua-derived transforming growth factor (TGF)-beta. These epithelial-trophoblast-like cells are therefore used in identifying genetic changes underlying epithelial tumor progression as well as gonadotrophin secretion studies.
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab14841 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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EMSA |
Use at an assay dependent dilution.
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WB |
Use at an assay dependent dilution.
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Notes |
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EMSA
Use at an assay dependent dilution. |
WB
Use at an assay dependent dilution. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab14841 has not yet been referenced specifically in any publications.