Jervine, Hedgehog (Hh) signaling inhibitor (ab120815)
- Datasheet
- References
Overview
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Product name
Jervine, Hedgehog (Hh) signaling inhibitor -
Description
Smoothened inhibitor -
Alternative names
- 11-Ketocyclopamine
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Biological description
Smoothened inhibitor (IC50 = 500-700 nM). Steroidal alkaloid. Prevents hedgehog signaling and therefore Gli1 transcription. -
Purity
> 98% -
CAS Number
469-59-0 -
Chemical structure
Properties
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Chemical name
(3β,23β)-17,23-Epoxy-3-hydroxyveratraman-11-one -
Molecular weight
425.61 -
Molecular formula
C27H39NO3 -
Storage instructions
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in DMSO to 10 mM and in ethanol to 10 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Toxic, refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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Source
Veratrum nigrum L.
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Research areas
Applications
Our Abpromise guarantee covers the use of ab120815 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Functional Studies | Use at an assay dependent concentration. |
Images
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Functional Studies - Jervine, Hedgehog (Hh) signaling inhibitor (ab120815)NIH-3T3 cells were incubated at 37°C for 7h with vehicle control (0 µM) and 5 µM of jervine (ab120815) in DMSO. Decreased expression of smoothened (ab38686) in NIH-3T3 cells correlates with an increase in jervine concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 20 µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 3% milk before being incubated with ab38686 at 1 µg /ml and ab8227 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.
References
This product has been referenced in:
- Williams JA et al. Identification of a small molecule inhibitor of the hedgehog signaling pathway: effects on basal cell carcinoma-like lesions. Proc Natl Acad Sci U S A 100:4616-21 (2003). Read more (PubMed: 12679522) »
- Incardona JP & Eaton S Cholesterol in signal transduction. Curr Opin Cell Biol 12:193-203 (2000). Read more (PubMed: 10712926) »
