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Stem Cells Signaling Pathways Hedgehog Surface Molecules
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Jervine, Hedgehog (Hh) signaling inhibitor (ab120815)

  • Datasheet
  • SDS
  • COA
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Functional Studies - Jervine, Hedgehog (Hh) signaling inhibitor (ab120815)

    Key features and details

    • Smoothened inhibitor
    • CAS Number: 469-59-0
    • Purity: > 98%
    • Soluble in DMSO to 10 mM and in ethanol to 10 mM
    • Form / State: Solid
    • Source: Veratrum nigrum L.

    Overview

    • Product name

      Jervine, Hedgehog (Hh) signaling inhibitor
    • Description

      Smoothened inhibitor
    • Alternative names

      • 11-Ketocyclopamine
    • Biological description

      Smoothened inhibitor (IC50 = 500-700 nM). Steroidal alkaloid. Prevents hedgehog signaling and therefore Gli1 transcription.

    • Purity

      > 98%
    • CAS Number

      469-59-0
    • Chemical structure

      Chemical Structure

    Properties

    • Chemical name

      (3β,23β)-17,23-Epoxy-3-hydroxyveratraman-11-one
    • Molecular weight

      425.61
    • Molecular formula

      C27H39NO3
    • Storage instructions

      Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
    • Solubility overview

      Soluble in DMSO to 10 mM and in ethanol to 10 mM
    • Handling

      Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

      Toxic, refer to SDS for further information.

      Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

    • Source

      Veratrum nigrum L.

    • Research areas

      • Stem Cells
      • Signaling Pathways
      • Hedgehog
      • Surface Molecules
      • Biochemicals
      • Chemical Type
      • Biochemicals
      • Biochemicals
      • Pharmacology
      • Receptors & Transporters
      • Smoothened
      • Biochemicals
      • Pharmacology
      • Signaling
      • Signal transduction
      • Hedgehog signaling
      • Inhibitors
      • Biochemicals
      • Research Area
      • Pain & inflammation
      • Signaling
      • Hedgehog signaling
      • Inhibitors
      • Biochemicals
      • Research Area
      • Alzheimer's Disease
      • Signaling
      • Signal transduction
      • Hedgehog signaling
      • Inhibitors
      • Biochemicals
      • Research Area
      • Diabetes
      • Signaling
      • Signal transduction
      • Hedgehog signaling
      • Inhibitors
      • Biochemicals
      • Research Area
      • Hypertension
      • Signaling
      • Signal transduction
      • Hedgehog signaling
      • Inhibitors
      • Biochemicals
      • Research Area
      • Obesity
      • Signaling
      • Signal transduction
      • Hedgehog signaling
      • Inhibitors
      • Biochemicals
      • Research Area
      • Respiratory disease
      • Signaling
      • Hedgehog signaling
      • Inhibitors
      • Biochemicals
      • Research Area
      • Stroke
      • Signaling
      • Heat Shock Proteins
      • Biochemicals
      • Pharmacology
      • Signaling
      • Stem Cells
      • Hedgehog signaling
      • Biochemicals
      • Research Area
      • Cancer
      • Signal transduction
      • Hedgehog signaling
      • Inhibitors
      • Biochemicals
      • Research Area
      • Stem Cells
      • Hedgehog signaling

    Applications

    Our Abpromise guarantee covers the use of ab120815 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Functional Studies Use at an assay dependent concentration.

    Images

    • Functional Studies - Jervine, Hedgehog (Hh) signaling inhibitor (ab120815)
      Functional Studies - Jervine, Hedgehog (Hh) signaling inhibitor (ab120815)

      NIH-3T3 cells were incubated at 37°C for 7h with vehicle control (0 µM) and 5 µM of jervine (ab120815) in DMSO. Decreased expression of smoothened (ab38686) in NIH-3T3 cells correlates with an increase in jervine concentration, as described in literature.

      Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 20 µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 3% milk before being incubated with ab38686 at 1 µg /ml and ab8227 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
    • COA
  • References (0)

    Publishing research using ab120815? Please let us know so that we can cite the reference in this datasheet.

    ab120815 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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