Anti-IB-2 antibody (ab154090)
Key features and details
- Rabbit polyclonal to IB-2
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-IB-2 antibody
See all IB-2 primary antibodies -
Description
Rabbit polyclonal to IB-2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow -
Immunogen
Recombinant fragment corresponding to Human IB-2 aa 529-804.
Database link: Q13387 -
Positive control
- 293T, A431, H1299, HeLa, HepG2, Molt4, Raji and mouse brain whole cell lysates; HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154090 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 88 kDa.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 88 kDa. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. JIP2 inhibits IL1 beta-induced apoptosis in insulin-secreting cells. May function as a regulator of vesicle transport, through interactions with the JNK-signaling components and motor proteins. -
Tissue specificity
Expressed mainly in the brain and pancreas, including insulin-secreting cells. In the nervous system, more abundantly expressed in the cerebellum, pituitary gland, occipital lobe and the amygdala. Also expressed in fetal brain. Very low levels found in uterus, ovary, prostate, colon, testis, adrenal gland, thyroid gland and salivary gland. -
Sequence similarities
Belongs to the JIP scaffold family.
Contains 1 PID domain.
Contains 1 SH3 domain. -
Cellular localization
Cytoplasm. Accumulates in cell surface projections. - Information by UniProt
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Database links
- Entrez Gene: 23542 Human
- Entrez Gene: 60597 Mouse
- Omim: 607755 Human
- SwissProt: Q13387 Human
- SwissProt: Q9ERE9 Mouse
- Unigene: 558180 Human
- Unigene: 173337 Mouse
- Unigene: 482169 Mouse
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Alternative names
- C jun amino terminal kinase interacting protein 2 antibody
- C-jun-amino-terminal kinase-interacting protein 2 antibody
- Homologous to mouse JIP 1 antibody
see all
Images
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Anti-IB-2 antibody (ab154090) at 1/1000 dilution + Molt4 whole cell lysate at 30 µg
Predicted band size: 88 kDa
7.5% SDS PAGE -
Anti-IB-2 antibody (ab154090) at 1/1000 dilution + mouse brain whole cell lysate at 50 µg
Predicted band size: 88 kDa
7.5% SDS PAGE -
Immunofluorescence analysis of methanol-fixed HeLa cells labeling IB-2 with ab154090 at 1/200 dilution. The image in the lower panel is costained with Hoechst 33342.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab154090 has been referenced in 2 publications.
- Goodwani S et al. Dual Leucine Zipper Kinase Is Constitutively Active in the Adult Mouse Brain and Has Both Stress-Induced and Homeostatic Functions. Int J Mol Sci 21:N/A (2020). WB ; Mouse . PubMed: 32659913
- Haidar M et al. miR-126-5p by direct targeting of JNK-interacting protein-2 (JIP-2) plays a key role in Theileria-infected macrophage virulence. PLoS Pathog 14:e1006942 (2018). PubMed: 29570727