• Product name
  • Description
    Rabbit polyclonal to JIP2
  • Host species
  • Tested applications
    Suitable for: WB, ELISA, ICC/IF, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Zebra finch
  • Immunogen

    A synthetic peptide derived from an internal of region human JIP2.

  • Positive control
    • Human brain tissue and extracts from mouse brain cells



Our Abpromise guarantee covers the use of ab65211 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 88 kDa (predicted molecular weight: 88 kDa).
ELISA 1/5000.
ICC/IF 1/500 - 1/1000.
IHC-P 1/50 - 1/100.
IHC-Fr 1/50.


  • Function
    The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. JIP2 inhibits IL1 beta-induced apoptosis in insulin-secreting cells. May function as a regulator of vesicle transport, through interactions with the JNK-signaling components and motor proteins.
  • Tissue specificity
    Expressed mainly in the brain and pancreas, including insulin-secreting cells. In the nervous system, more abundantly expressed in the cerebellum, pituitary gland, occipital lobe and the amygdala. Also expressed in fetal brain. Very low levels found in uterus, ovary, prostate, colon, testis, adrenal gland, thyroid gland and salivary gland.
  • Sequence similarities
    Belongs to the JIP scaffold family.
    Contains 1 PID domain.
    Contains 1 SH3 domain.
  • Cellular localization
    Cytoplasm. Accumulates in cell surface projections.
  • Information by UniProt
  • Database links
  • Alternative names
    • C jun amino terminal kinase interacting protein 2 antibody
    • C-jun-amino-terminal kinase-interacting protein 2 antibody
    • Homologous to mouse JIP 1 antibody
    • IB 2 antibody
    • IB-2 antibody
    • IB2 antibody
    • Islet brain 2 antibody
    • Islet-brain-2 antibody
    • JIP 2 antibody
    • JIP-2 antibody
    • JIP2 antibody
    • JIP2_HUMAN antibody
    • JNK interacting protein 2 antibody
    • JNK MAP kinase scaffold protein 2 antibody
    • JNK MAP kinase scaffold protein JIP2 antibody
    • JNK-interacting protein 2 antibody
    • MAPK8IP2 antibody
    • Mitogen activated protein kinase 8 interacting protein 2 antibody
    • Mitogen-activated protein kinase 8-interacting protein 2 antibody
    • PRKM8 interacting protein like antibody
    • PRKM8IPL antibody
    see all


  • All lanes : Anti-JIP2 antibody (ab65211) at 1/500 dilution

    Lane 1 : Extracts from mouse brain cells
    Lane 2 : Extracts from mouse brain cells, plus immunising peptide

    Predicted band size: 88 kDa
    Observed band size: 88 kDa
    Additional bands at: 30 kDa. We are unsure as to the identity of these extra bands.

    The amount of positive control loading for the WB is 5-30 ug of total protein.
    The amount of the peptide for the WB is 5-10 ug.
  • ab65211 staining the JIP2 (Red) in Zebra finch basal ganglia (striatum) tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with TBS + 0.3% Triton and blocked with 5% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/50 in PBS Tween) for 24 hours at 4°C. An Alexa Fluor®555-conjugated goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody. Nuclei were stained with DAPI (Blue).

    See Abreview

  • ICC/IF image of ab65211 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65211, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


ab65211 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Immunohistochemistry (Frozen sections)
Zebra finch Tissue sections (basal ganglia (striatum)-fixed frozen sections)
basal ganglia (striatum)-fixed frozen sections
Yes - TBS w/0.3% Triton
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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Submitted Jun 03 2010


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