• Product name
  • Description
    Goat polyclonal to JIP3
  • Host species
  • Specificity
    This antibody is expected to recognise both human isoforms of this protein.
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Cow, Human
  • Immunogen

    Synthetic peptide:


    , corresponding to N terminal amino acids 2-13 of Human JIP3.

  • General notes
    GenBank Accession Number – NP_055948; NP_203750. LocusLink ID - 23162 (human); 30957 (mouse). Gene Ontology terms - vesicle-mediated transport; kinesin complex; cytoplasm; kinesin binding; MAP-kinase scaffold activity; protein kinase binding; regulation of JNK cascade.



Our Abpromise guarantee covers the use of ab4545 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
IHC-P Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • Function
    The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. May function as a regulator of vesicle transport, through interations with the JNK-signaling components and motor proteins.
  • Sequence similarities
    Belongs to the JIP scaffold family.
  • Post-translational
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • C-jun-amino-terminal kinase-interacting protein 3 antibody
    • FLJ00027 antibody
    • Homolog of Drosophila Sunday driver 2 antibody
    • JIP-3 antibody
    • JIP3 antibody
    • JIP3_HUMAN antibody
    • JNK / Stress Activated Protein Kinase Associated Protein 1 antibody
    • JNK Interacting Protein 3 antibody
    • JNK MAP kinase scaffold protein 3 antibody
    • JNK-interacting protein 3 antibody
    • JNK/SAPK associated protein 1 antibody
    • JSAP1 antibody
    • KIAA1066 antibody
    • MAPK8IP3 antibody
    • Mitogen Activated Protein kinase 8 Interacting Protein 3 antibody
    • Mitogen-activated protein kinase 8-interacting protein 3 antibody
    • Syd antibody
    • SYD2 antibody
    see all


  • ab4545 (4µg/ml) staining of paraffin embedded Mouse Brain. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining. Shows axonal staining in various regions


ab4545 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Western blot
Rabbit Tissue lysate - whole (heart)
Loading amount
60 µg
Gel Running Conditions
Non-reduced Denaturing (7.5)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Feb 15 2012


Thank you for your email. To ship to Canada, the cost is 60 USD (United States Dollar). If you have any more questions, please let me know.

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Thank you for your email. We have not yet received feedback regarding this antibody so it is still possible for you to obtain the $300 Conference Travel Award. A discount is not available for this antibody. If you have any more questions, just let us know.

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Thank you for your enquiry. Ab4545 is a Fast-Track antibody; Fast-Track antibodies are immunogen affinity purified and are shown by ELISA to have high titre values against their immunogen. We are currently testing them for use in Western blotting. At this stage, there can be delays. We sometimes experience problems in searching for / developing the correct lysate or knock-out cell-line. In addition, certain products can be difficult to characterise, due, for instance, to low expression levels or a lack of expression in easily available lysates. More details regarding the Fast-Track antibody terms of use can be found on the online datasheet for ab4545. Ab4545 has been tested in peptide ELISA, antibody detection limit at a dilution of 1/4000. In Western blotting, no signal has been obtained yet, but low background was observed in Human Heart lysate at up to 2µg/ml. We don't have an image available at this time or any additional information. If you choose to purchase this antibody, we would greatly appreciate your feedback on these antibodies, whether positive or negative. We will award $300 of Conference Travel Money to the first researcher who sends us positive feedback (including an image and details of materials & methods). Conclusive negative feedback that leads us to withdraw the antibody will also be rewarded with $300 of Conference Travel Money. Ab4545 is currently out of stock; if you placed an order tomorrow, you would get it in approximately 2-3 weeks time. If you have any more questions, please contact us again.

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