Anti-JLP antibody (ab12331)
Key features and details
- Rabbit polyclonal to JLP
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-JLP antibody
See all JLP primary antibodies -
Description
Rabbit polyclonal to JLP -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Opossum, Orangutan, Elephant -
Immunogen
Synthetic peptide within Human JLP aa 1-100. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: O60271 -
Positive control
- IP: HeLa nuclear extract. WB: HeLa, 293T,Jurkat, 3T3 cell lysates IHC-P: Human testis, and seminoma tissues
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
This antibody is affinity purified. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab12331 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 156.8 kDa.
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IP |
Use a concentration of 2 - 5 µg/ml.
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Notes |
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IHC-P
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/1000 - 1/10000. Predicted molecular weight: 156.8 kDa. |
IP
Use a concentration of 2 - 5 µg/ml. |
Target
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Relevance
JLP is a member of the JNK-interacting protein (JIP) group of scaffold proteins which selectively mediate JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. -
Cellular localization
Cytoplasm. Cytoplasm, perinuclear region. Note: Perinuclear distribution in response to stress signals such as UV radiation. -
Database links
- Entrez Gene: 9043 Human
- Entrez Gene: 70834 Mouse
- Omim: 605430 Human
- SwissProt: O60271 Human
- SwissProt: Q58A65 Mouse
- SwissProt: Q8CJC2 Mouse
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Alternative names
- C jun amino terminal kinase interacting protein 4 antibody
- c Jun NH2 terminal kinase associated leucine zipper protein antibody
- FLJ13450 antibody
see all
Images
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All lanes : Anti-JLP antibody (ab12331) at 0.1 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : 3T3 cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 156.8 kDa -
Immunoprecipitation analysis of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded). ab12331 was used for immunoprecipitation at 3 µg/mg lysate and used for western blot at 1 µg/ml. Lanes 1 and 2 - ab12331 IP, Lane 3 - control IgG.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of Human seminoma using ab12331 at 1/1000 with and HRP- conjugated goat anti-rabbit secondary antibody.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (21)
ab12331 has been referenced in 21 publications.
- Tian M et al. Loss of JNK-Associated Leucine Zipper Protein Promotes Peritoneal Dialysis-Related Peritoneal Fibrosis. Kidney Dis (Basel) 8:168-179 (2022). PubMed: 35527988
- Okumura F et al. ZSWIM8 is a myogenic protein that partly prevents C2C12 differentiation. Sci Rep 11:20880 (2021). PubMed: 34686700
- Yan Q et al. A negative feedback loop between JNK-associated leucine zipper protein and TGF-ß1 regulates kidney fibrosis. Commun Biol 3:288 (2020). PubMed: 32504044
- Yan P et al. LncRNA NEAT1 enhances the resistance of anaplastic thyroid carcinoma cells to cisplatin by sponging miR-9-5p and regulating SPAG9 expression. Int J Oncol 55:988-1002 (2019). PubMed: 31485599
- Luo S et al. SPAG9/MKK3/p38 axis is a novel therapeutic target for liver cancer. Oncol Rep 41:2329-2336 (2019). PubMed: 30720101