For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    jlp-antibody-ab12332.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Epigenetics and Nuclear Signaling Transcription Domain Families HLH / Leucine Zipper Leucine Zipper
Share by email

Anti-JLP antibody (ab12332)

  • Datasheet
  • SDS
Submit a review Submit a question References (1)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JLP antibody (ab12332)
  • Western blot - Anti-JLP antibody (ab12332)
  • Western blot - Anti-JLP antibody (ab12332)
  • Western blot - Anti-JLP antibody (ab12332)
  • Immunoprecipitation - Anti-JLP antibody (ab12332)

Key features and details

  • Rabbit polyclonal to JLP
  • Suitable for: IHC-P, WB, IP
  • Reacts with: Mouse, Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-JLP antibody
    See all JLP primary antibodies
  • Description

    Rabbit polyclonal to JLP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Chimpanzee, Baboon, Rhesus monkey, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human JLP aa 775-825.
    Database link: O60271

  • Positive control

    • HeLa nuclear extract.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7
    Preservative: 0.1% Sodium azide
    Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    This antibody is affinity purified.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • HLH / Leucine Zipper
    • Leucine Zipper
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Enzymes
    • Other Enzymes
    • Stem Cells
    • Germline Stem Cells
    • Embryonic Germ Cells
    • Developmental Biology
    • Reproduction
    • Germ cell markers

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab12332 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/1000 - 1/10000. Predicted molecular weight: 156.8 kDa.
IP
Use at 6 µg/mg of lysate.
Notes
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/1000 - 1/10000. Predicted molecular weight: 156.8 kDa.
IP
Use at 6 µg/mg of lysate.

Target

  • Relevance

    JLP is a member of the JNK-interacting protein (JIP) group of scaffold proteins which selectively mediate JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module.
  • Cellular localization

    Cytoplasm. Cytoplasm, perinuclear region. Note: Perinuclear distribution in response to stress signals such as UV radiation.
  • Database links

    • Entrez Gene: 9043 Human
    • Entrez Gene: 70834 Mouse
    • Omim: 605430 Human
    • SwissProt: O60271 Human
    • SwissProt: Q58A65 Mouse
    • SwissProt: Q8CJC2 Mouse
    • Alternative names

      • C jun amino terminal kinase interacting protein 4 antibody
      • c Jun NH2 terminal kinase associated leucine zipper protein antibody
      • FLJ13450 antibody
      • FLJ14006 antibody
      • FLJ34602 antibody
      • HLC 4 antibody
      • HLC 4 protein antibody
      • HLC 6 antibody
      • HLC4 antibody
      • HLC4 protein antibody
      • HLC6 antibody
      • HSS antibody
      • Human lung cancer protein 6 antibody
      • JIP 4 antibody
      • JIP4 antibody
      • JNK associated leucine zipper protein 1 antibody
      • JNK associated leucine zipper protein antibody
      • JNK interacting protein 4 antibody
      • JNK interacting protein antibody
      • JNK/SAPK associated protein antibody
      • KIAA0516 antibody
      • Lung cancer oncogene 4 antibody
      • MAPK8IP4 antibody
      • Max binding protein antibody
      • MGC117291 antibody
      • MGC14967 antibody
      • MGC74461 antibody
      • Mitogen activated protein kinase 8 interacting protein 4 antibody
      • PHET antibody
      • PIG 6 antibody
      • PIG6 antibody
      • Proliferation inducing gene 6 antibody
      • Protein highly expressed in testis antibody
      • SPAG 9 antibody
      • SPAG9 antibody
      • Sperm associated antigen 9 antibody
      • Sperm specific protein antibody
      • Sperm surface protein antibody
      • Sunday driver (drosophila) homolog antibody
      • Sunday driver 1 antibody
      • SYD 1 antibody
      • SYD1 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JLP antibody (ab12332)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JLP antibody (ab12332)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma tissue labelling JLP with ab12332 at 1/1000 (1µg/ml). Detection: DAB.
    • Western blot - Anti-JLP antibody (ab12332)
      Western blot - Anti-JLP antibody (ab12332)
      All lanes : Anti-JLP antibody (ab12332) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : 293T whole cell lysate at 50 µg

      Developed using the ECL technique.

      Predicted band size: 156.8 kDa


      Exposure time: 30 seconds
    • Western blot - Anti-JLP antibody (ab12332)
      Western blot - Anti-JLP antibody (ab12332)
      All lanes : Anti-JLP antibody (ab12332) at 0.1 µg/ml

      Lane 1 : TCKM whole cell lysate at 50 µg
      Lane 2 : Renca whole cell lysate at 15 µg
      Lane 3 : CT26 whole cell lysate at 50 µg

      Developed using the ECL technique.

      Predicted band size: 156.8 kDa


      Exposure time: 10 seconds
    • Western blot - Anti-JLP antibody (ab12332)
      Western blot - Anti-JLP antibody (ab12332)

      Samples:

      Nuclear extract (NE, 20 µg) from HeLa cells. Antibody: Affinity purified rabbit anti-JLP antibody ab12332 used at 0.3 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

       

      Samples: Nuclear extract (NE, 20 µg) from HeLa cells. Antibody: Affinity purified rabbit anti-JLP antibody ab12332 used at 0.3 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
    • Immunoprecipitation - Anti-JLP antibody (ab12332)
      Immunoprecipitation - Anti-JLP antibody (ab12332)

      Immunoprecipitation analaysis of HeLa whole cell lysate (1 mg for IP; 20% of IP loaded). ab12332 was used for immunoprecipitation at 6 µg/mg lysate (lanes 1 and 2). Lane 3 - control IgG. For western blot ab12332 was used at 1 µg/ml.

    Protocols

    • Western blot protocols
    • Immunoprecipitation protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab12332? Please let us know so that we can cite the reference in this datasheet.

    ab12332 has been referenced in 1 publication.

    • Blüher M  et al. Activated Ask1-MKK4-p38MAPK/JNK stress signaling pathway in human omental fat tissue may link macrophage infiltration to whole-body Insulin sensitivity. J Clin Endocrinol Metab 94:2507-15 (2009). PubMed: 19351724

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab12332.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2021 Abcam plc. All rights reserved.