Anti-JLP antibody (ab12332)
Key features and details
- Rabbit polyclonal to JLP
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-JLP antibody
See all JLP primary antibodies -
Description
Rabbit polyclonal to JLP -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Chimpanzee, Baboon, Rhesus monkey, Orangutan
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Immunogen
Synthetic peptide corresponding to Human JLP aa 775-825.
Database link: O60271 -
Positive control
- HeLa nuclear extract.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This antibody is affinity purified. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab12332 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
1/1000 - 1/10000. Predicted molecular weight: 156.8 kDa.
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| IP |
Use at 6 µg/mg of lysate.
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| Notes |
|---|
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IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
1/1000 - 1/10000. Predicted molecular weight: 156.8 kDa. |
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IP
Use at 6 µg/mg of lysate. |
Target
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Relevance
JLP is a member of the JNK-interacting protein (JIP) group of scaffold proteins which selectively mediate JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. -
Cellular localization
Cytoplasm. Cytoplasm, perinuclear region. Note: Perinuclear distribution in response to stress signals such as UV radiation. -
Database links
- Entrez Gene: 9043 Human
- Entrez Gene: 70834 Mouse
- Omim: 605430 Human
- SwissProt: O60271 Human
- SwissProt: Q58A65 Mouse
- SwissProt: Q8CJC2 Mouse
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Alternative names
- C jun amino terminal kinase interacting protein 4 antibody
- c Jun NH2 terminal kinase associated leucine zipper protein antibody
- FLJ13450 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JLP antibody (ab12332)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma tissue labelling JLP with ab12332 at 1/1000 (1µg/ml). Detection: DAB.
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Western blot - Anti-JLP antibody (ab12332)All lanes : Anti-JLP antibody (ab12332) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 156.8 kDa
Exposure time: 30 seconds -
Western blot - Anti-JLP antibody (ab12332)All lanes : Anti-JLP antibody (ab12332) at 0.1 µg/ml
Lane 1 : TCKM whole cell lysate at 50 µg
Lane 2 : Renca whole cell lysate at 15 µg
Lane 3 : CT26 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 156.8 kDa
Exposure time: 10 seconds -
Western blot - Anti-JLP antibody (ab12332)Samples:
Nuclear extract (NE, 20µ µ
Samples: Nuclear extract (NE, 20 µg) from HeLa cells. Antibody: Affinity purified rabbit anti-JLP antibody ab12332 used at 0.3 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds. -
Immunoprecipitation - Anti-JLP antibody (ab12332)Immunoprecipitation analaysis of HeLa whole cell lysate (1 mg for IP; 20% of IP loaded). ab12332 was used for immunoprecipitation at 6 µg/mg lysate (lanes 1 and 2). Lane 3 - control IgG. For western blot ab12332 was used at 1 µg/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab12332 has been referenced in 1 publication.
- Blüher M et al. Activated Ask1-MKK4-p38MAPK/JNK stress signaling pathway in human omental fat tissue may link macrophage infiltration to whole-body Insulin sensitivity. J Clin Endocrinol Metab 94:2507-15 (2009). PubMed: 19351724