Overview

  • Product name
  • Description
    Rabbit polyclonal to JM4
  • Host species
    Rabbit
  • Specificity
    ab53113 detects endogenous levels of total JM4 protein.
  • Tested applications
    Suitable for: IHC-P, ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    A synthetic peptide derived from human JM4.

  • Positive control
    • Human brain tissue and extracts from LOVO cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS

    Without Mg+2 and Ca+2
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab53113 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
ELISA 1/40000.
WB 1/300 - 1/1000. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).

Target

  • Function
    May be involved in ER/Golgi transport and vesicular traffic. Plays a proapoptic role in cerulenin-induced neuroblastoma apoptosis.
  • Tissue specificity
    Strong expression in the brain, small intestine, lung, spleen, and pancreas as well as in tumor tissues of the breast, colon, lung and ovary, with a weaker expression in normal tissues of the same patient. High expression in neuroblastic tumors. Strongly expressed in Purkinje cells and more moderately in cells of the molecular and the granular layers in the cerebellum. Detected in neuronal cells, but not in non-neuronal cells in the cerebral cortex, hippocampus, and lateral ventricles.
  • Sequence similarities
    Belongs to the PRA1 family.
  • Cellular localization
    Endosome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Jena Muenchen 4 antibody
    • JM 4 antibody
    • PRA1 domain family member 2 antibody
    • PRA1 family protein 2 antibody
    • PRAF2 antibody
    • PRAF2_HUMAN antibody
    see all

Images

  • All lanes : Anti-JM4 antibody (ab53113) at 1/300 dilution

    Lane 1 : Mouse spleen whole tissue lysate
    Lane 2 : Mouse braint whole tissue lysate
    Lane 3 : Mouse heart whole tissue lysate
    Lane 4 : Mouse kidney whole tissue lysate
    Lane 5 : Mouse liver whole tissue lysate
    Lane 6 : Mouse embryonic fibroblasts lysate

    Lysates/proteins at 40 µg per lane.

    Secondary
    All lanes : HRP-conjugated Goat anti-rabbit at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 19 kDa
    Observed band size: 22 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 55 kDa (possible non-specific binding)


    Exposure time: 20 seconds

    See Abreview

  • All lanes : Anti-JM4 antibody (ab53113) at 1/300 dilution

    Lane 1 : LOVO cell extract
    Lane 2 : LOVO cell extract with immunising peptide

    Predicted band size: 19 kDa
    Observed band size: 19 kDa

  • ab53113 at 1/50 dilution staining JM4 in human brain tissue by Immunohistochemistry, Paraffin embedded tissue, in the absence and presence of the immunising peptide.

References

This product has been referenced in:
  • Chiu HC  et al. High-throughput quantitative proteomic analysis of dengue virus type 2 infected A549 cells. PLoS One 9:e93305 (2014). WB ; Human . Read more (PubMed: 24671231) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Perfused/frozen brain slices)
Permeabilization
Yes - 0.2% TX-100
Specification
Perfused/frozen brain slices
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative
Formaldehyde

Mrs. Sandra Braz

Verified customer

Submitted Sep 25 2018

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (12,5)
Sample
Mouse Tissue lysate - whole (Spleen, Liver, Heart, Brain, kidney)
Specification
Spleen, Liver, Heart, Brain, kidney
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 2%

Abcam user community

Verified customer

Submitted Sep 16 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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