Anti-JMJD1A antibody (ab106456)
Key features and details
- Rabbit polyclonal to JMJD1A
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-JMJD1A antibody -
Description
Rabbit polyclonal to JMJD1A -
Host species
Rabbit -
Specificity
ab106456 does not not cross-react with JMJD1B or JMJD1C. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF RatIHC-P RatWB Human
-
Immunogen
Synthetic peptide corresponding to Human JMJD1A (N terminal).
Database link: Q9Y4C1 -
Positive control
- Mouse liver tissue lysate, 293, A549, HeLa, HepG2, MCF-7 and SK-N-SH cell lysates; Rat liver tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at 4°C (stable for up to 12 months). -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab106456 is purified by affinity chromatography via a peptide column. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab106456 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Guaranteed
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted
Predicted to work for this combination of applications and species but not guaranteed.
Incompatible
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Rat
|
| IHC-P |
Rat
|
| WB |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use a concentration of 20 µg/ml.
|
|
| WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 147 kDa.
|
|
| IHC-P |
Use a concentration of 5 µg/ml.
|
| Notes |
|---|
|
ICC/IF
Use a concentration of 20 µg/ml. |
|
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 147 kDa. |
|
IHC-P
Use a concentration of 5 µg/ml. |
Target
-
Relevance
This gene encodes a zinc finger protein that contains a jumonji domain and may play a role in hormone-dependent transcriptional activation. Alternative splicing results in multiple transcript variants. -
Cellular localization
Cytoplasmic and Nuclear -
Database links
- Entrez Gene: 55818 Human
- Entrez Gene: 312440 Rat
- Omim: 611512 Human
- SwissProt: Q9Y4C1 Human
- SwissProt: Q63679 Rat
-
Alternative names
- JHDM2A antibody
- JmjC domain containing histone demethylation protein 2A antibody
- JMJD1 antibody
see all
Images
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Western blot - Anti-JMJD1A antibody (ab106456)All lanes : Anti-JMJD1A antibody (ab106456) at 2 µg
Lane 1 : 293 cell lysate
Lane 2 : A549 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : HepG2 cell lysate
Lane 5 : MCF-7 cell lysate
Lane 6 : SK-N-SH cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 147 kDa -
Western blot - Anti-JMJD1A antibody (ab106456)Anti-JMJD1A antibody (ab106456) at 1 µg/ml + HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Predicted band size: 147 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JMJD1A antibody (ab106456)ab106456, at 5 µg/ml staining JMJD1A in Rat liver tissue by Immunohistochemistry, Paraffin-embedded tissue. -
Immunocytochemistry/ Immunofluorescence - Anti-JMJD1A antibody (ab106456)Immunofluorescence of JMJD1A in Rat Liver cells using ab106456 at 20 ug/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab106456 has been referenced in 7 publications.
- Wang T et al. Dihydroartemisinin suppresses bladder cancer cell invasion and migration by regulating KDM3A and p21. J Cancer 11:1115-1124 (2020). PubMed: 31956358
- Ding H et al. Upregulation of miR-101a Suppresses Chronic Renal Fibrosis by Regulating KDM3A via Blockade of the YAP-TGF-ß-Smad Signaling Pathway. Mol Ther Nucleic Acids 19:1276-1289 (2020). PubMed: 32092824
- Zang R et al. JMJD1A Represses the Development of Cardiomyocyte Hypertrophy by Regulating the Expression of Catalase. Biomed Res Int 2020:5081323 (2020). PubMed: 32461996
- Cui X et al. ZNFX1 anti-sense RNA 1 promotes the tumorigenesis of prostate cancer by regulating c-Myc expression via a regulatory network of competing endogenous RNAs. Cell Mol Life Sci N/A:N/A (2019). PubMed: 31321444
- Huang B et al. KDM3A and KDM4C Regulate Mesenchymal Stromal Cell Senescence and Bone Aging via Condensin-mediated Heterochromatin Reorganization. iScience 21:375-390 (2019). PubMed: 31704649
- Zhang BF et al. KDM3A inhibition attenuates high concentration insulin-induced vascular smooth muscle cell injury by suppressing MAPK/NF-?B pathways. Int J Mol Med 41:1265-1274 (2018). PubMed: 29286083
- Shi ZH et al. Mitochondrial ferritin, a new target for inhibiting neuronal tumor cell proliferation. Cell Mol Life Sci N/A:N/A (2014). WB . PubMed: 25213357