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    jmjd1a-antibody-ab106456.pdf

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Epigenetics and Nuclear Signaling Histones H1 Methylated
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Anti-JMJD1A antibody (ab106456)

  • Datasheet
  • SDS
Submit a review Submit a question References (7)

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Western blot - Anti-JMJD1A antibody (ab106456)
  • Western blot - Anti-JMJD1A antibody (ab106456)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JMJD1A antibody (ab106456)
  • Immunocytochemistry/ Immunofluorescence - Anti-JMJD1A antibody (ab106456)

Key features and details

  • Rabbit polyclonal to JMJD1A
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Rat, Human
  • Isotype: IgG

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Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Primary
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Anti-KDM6B / JMJD3 antibody (ab38113)

View more associated products

Overview

  • Product name

    Anti-JMJD1A antibody
  • Description

    Rabbit polyclonal to JMJD1A
  • Host species

    Rabbit
  • Specificity

    ab106456 does not not cross-react with JMJD1B or JMJD1C.
  • Tested Applications & Species

    Application Species
    ICC/IF
    Rat
    IHC-P
    Rat
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human JMJD1A (N terminal).
    Database link: Q9Y4C1

  • Positive control

    • Mouse liver tissue lysate, 293, A549, HeLa, HepG2, MCF-7 and SK-N-SH cell lysates; Rat liver tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at 4°C (stable for up to 12 months).
  • Storage buffer

    pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab106456 is purified by affinity chromatography via a peptide column.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Histones
    • H1
    • Methylated

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab106456 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Rat
IHC-P
Rat
WB
Human
Application Abreviews Notes
ICC/IF
Use a concentration of 20 µg/ml.
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 147 kDa.
IHC-P
Use a concentration of 5 µg/ml.
Notes
ICC/IF
Use a concentration of 20 µg/ml.
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 147 kDa.
IHC-P
Use a concentration of 5 µg/ml.

Target

  • Relevance

    This gene encodes a zinc finger protein that contains a jumonji domain and may play a role in hormone-dependent transcriptional activation. Alternative splicing results in multiple transcript variants.
  • Cellular localization

    Cytoplasmic and Nuclear
  • Database links

    • Entrez Gene: 55818 Human
    • Entrez Gene: 312440 Rat
    • Omim: 611512 Human
    • SwissProt: Q9Y4C1 Human
    • SwissProt: Q63679 Rat
    • Alternative names

      • JHDM2A antibody
      • JmjC domain containing histone demethylation protein 2A antibody
      • JMJD1 antibody
      • Jumonji C domain containing histone demethylase 2A antibody
      • Jumonji domain containing 1 antibody
      • Jumonji domain containing 1A antibody
      • Jumonji domain containing protein 1A antibody
      • KDM3A antibody
      • KIAA0742 antibody
      • Lysine (K) specific demethylase 3A antibody
      • Lysine specific demethylase 3A antibody
      • Testis specific protein A antibody
      • TSGA antibody
      see all

    Images

    • Western blot - Anti-JMJD1A antibody (ab106456)
      Western blot - Anti-JMJD1A antibody (ab106456)
      All lanes : Anti-JMJD1A antibody (ab106456) at 2 µg

      Lane 1 : 293 cell lysate
      Lane 2 : A549 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : HepG2 cell lysate
      Lane 5 : MCF-7 cell lysate
      Lane 6 : SK-N-SH cell lysate

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

      Predicted band size: 147 kDa

    • Western blot - Anti-JMJD1A antibody (ab106456)
      Western blot - Anti-JMJD1A antibody (ab106456)
      Anti-JMJD1A antibody (ab106456) at 1 µg/ml + HepG2 (human liver hepatocellular carcinoma cell line) cell lysate

      Predicted band size: 147 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JMJD1A antibody (ab106456)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JMJD1A antibody (ab106456)
      ab106456, at 5 µg/ml staining JMJD1A in Rat liver tissue by Immunohistochemistry, Paraffin-embedded tissue.
    • Immunocytochemistry/ Immunofluorescence - Anti-JMJD1A antibody (ab106456)
      Immunocytochemistry/ Immunofluorescence - Anti-JMJD1A antibody (ab106456)
      Immunofluorescence of JMJD1A in Rat Liver cells using ab106456 at 20 ug/ml.

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (7)

    Publishing research using ab106456? Please let us know so that we can cite the reference in this datasheet.

    ab106456 has been referenced in 7 publications.

    • Wang T  et al. Dihydroartemisinin suppresses bladder cancer cell invasion and migration by regulating KDM3A and p21. J Cancer 11:1115-1124 (2020). PubMed: 31956358
    • Ding H  et al. Upregulation of miR-101a Suppresses Chronic Renal Fibrosis by Regulating KDM3A via Blockade of the YAP-TGF-ß-Smad Signaling Pathway. Mol Ther Nucleic Acids 19:1276-1289 (2020). PubMed: 32092824
    • Zang R  et al. JMJD1A Represses the Development of Cardiomyocyte Hypertrophy by Regulating the Expression of Catalase. Biomed Res Int 2020:5081323 (2020). PubMed: 32461996
    • Cui X  et al. ZNFX1 anti-sense RNA 1 promotes the tumorigenesis of prostate cancer by regulating c-Myc expression via a regulatory network of competing endogenous RNAs. Cell Mol Life Sci N/A:N/A (2019). PubMed: 31321444
    • Huang B  et al. KDM3A and KDM4C Regulate Mesenchymal Stromal Cell Senescence and Bone Aging via Condensin-mediated Heterochromatin Reorganization. iScience 21:375-390 (2019). PubMed: 31704649
    • Zhang BF  et al. KDM3A inhibition attenuates high concentration insulin-induced vascular smooth muscle cell injury by suppressing MAPK/NF-?B pathways. Int J Mol Med 41:1265-1274 (2018). PubMed: 29286083
    • Shi ZH  et al. Mitochondrial ferritin, a new target for inhibiting neuronal tumor cell proliferation. Cell Mol Life Sci N/A:N/A (2014). WB . PubMed: 25213357

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