Anti-JMJD1A antibody (ab106456)
- Datasheet
- References (3)
- Protocols
Overview
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Product name
Anti-JMJD1A antibody -
Description
Rabbit polyclonal to JMJD1A -
Host species
Rabbit -
Specificity
ab106456 does not not cross-react with JMJD1B or JMJD1C. -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human JMJD1A (N terminal).
Database link: Q9Y4C1 -
Positive control
- Mouse liver tissue lysate; Rat liver tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at 4°C (stable for up to 12 months). -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab106456 is purified by affinity chromatography via a peptide column. -
Clonality
Polyclonal -
Isotype
IgG
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab106456 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 20 µg/ml. | |
| WB | Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 147 kDa. | |
| IHC-P | Use a concentration of 5 µg/ml. |
Target
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Relevance
This gene encodes a zinc finger protein that contains a jumonji domain and may play a role in hormone-dependent transcriptional activation. Alternative splicing results in multiple transcript variants. -
Cellular localization
Cytoplasmic and Nuclear -
Database links
- Entrez Gene: 55818 Human
- Entrez Gene: 104263 Mouse
- Entrez Gene: 312440 Rat
- Omim: 611512 Human
- SwissProt: Q9Y4C1 Human
- SwissProt: Q6PCM1 Mouse
- SwissProt: Q63679 Rat
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Alternative names
- JHDM2A antibody
- JmjC domain containing histone demethylation protein 2A antibody
- JMJD1 antibody
see all
Images
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Western blot - Anti-JMJD1A antibody (ab106456)Anti-JMJD1A antibody (ab106456) at 1 µg/ml + HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Predicted band size: 147 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JMJD1A antibody (ab106456)ab106456, at 5 µg/ml staining JMJD1A in Rat liver tissue by Immunohistochemistry, Paraffin-embedded tissue. -
Immunocytochemistry/ Immunofluorescence - Anti-JMJD1A antibody (ab106456)Immunofluorescence of JMJD1A in Rat Liver cells using ab106456 at 20 ug/ml.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Cui X et al. ZNFX1 anti-sense RNA 1 promotes the tumorigenesis of prostate cancer by regulating c-Myc expression via a regulatory network of competing endogenous RNAs. Cell Mol Life Sci N/A:N/A (2019). Read more (PubMed: 31321444) »
- Zhang BF et al. KDM3A inhibition attenuates high concentration insulin-induced vascular smooth muscle cell injury by suppressing MAPK/NF-?B pathways. Int J Mol Med 41:1265-1274 (2018). Read more (PubMed: 29286083) »
