Anti-JMJD1A antibody (ab106456)
Key features and details
- Rabbit polyclonal to JMJD1A
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-JMJD1A antibody -
Description
Rabbit polyclonal to JMJD1A -
Host species
Rabbit -
Specificity
ab106456 does not not cross-react with JMJD1B or JMJD1C. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human JMJD1A (N terminal).
Database link: Q9Y4C1 -
Positive control
- WB: 293, A549, HeLa, HepG2, MCF-7 and SK-N-SH cell lysates;
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at 4°C (stable for up to 12 months). -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab106456 is purified by affinity chromatography via a peptide column. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab106456 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 147 kDa.
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Notes |
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 147 kDa. |
Target
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Relevance
This gene encodes a zinc finger protein that contains a jumonji domain and may play a role in hormone-dependent transcriptional activation. Alternative splicing results in multiple transcript variants. -
Cellular localization
Cytoplasmic and Nuclear -
Database links
- Entrez Gene: 55818 Human
- Omim: 611512 Human
- SwissProt: Q9Y4C1 Human
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Alternative names
- JHDM2A antibody
- JmjC domain containing histone demethylation protein 2A antibody
- JMJD1 antibody
see all
Images
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All lanes : Anti-JMJD1A antibody (ab106456) at 2 µg
Lane 1 : 293 cell lysate
Lane 2 : A549 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : HepG2 cell lysate
Lane 5 : MCF-7 cell lysate
Lane 6 : SK-N-SH cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 147 kDa -
Anti-JMJD1A antibody (ab106456) at 1 µg/ml + HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Predicted band size: 147 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab106456 has been referenced in 9 publications.
- Guo X et al. The histone demthylase KDM3A protects the myocardium from ischemia/reperfusion injury via promotion of ETS1 expression. Commun Biol 5:270 (2022). PubMed: 35338235
- Ding H et al. Upregulation of miR-101a Suppresses Chronic Renal Fibrosis by Regulating KDM3A via Blockade of the YAP-TGF-ß-Smad Signaling Pathway. Mol Ther Nucleic Acids 19:1276-1289 (2020). PubMed: 32092824
- Zang R et al. JMJD1A Represses the Development of Cardiomyocyte Hypertrophy by Regulating the Expression of Catalase. Biomed Res Int 2020:5081323 (2020). PubMed: 32461996
- Wang T et al. Dihydroartemisinin suppresses bladder cancer cell invasion and migration by regulating KDM3A and p21. J Cancer 11:1115-1124 (2020). PubMed: 31956358
- Huang B et al. KDM3A and KDM4C Regulate Mesenchymal Stromal Cell Senescence and Bone Aging via Condensin-mediated Heterochromatin Reorganization. iScience 21:375-390 (2019). PubMed: 31704649
- Liu X et al. KDM3A inhibition modulates macrophage polarization to aggravate post-MI injuries and accelerates adverse ventricular remodeling via an IRF4 signaling pathway. Cell Signal 64:109415 (2019). PubMed: 31513837
- Cui X et al. ZNFX1 anti-sense RNA 1 promotes the tumorigenesis of prostate cancer by regulating c-Myc expression via a regulatory network of competing endogenous RNAs. Cell Mol Life Sci N/A:N/A (2019). PubMed: 31321444
- Zhang BF et al. KDM3A inhibition attenuates high concentration insulin-induced vascular smooth muscle cell injury by suppressing MAPK/NF-?B pathways. Int J Mol Med 41:1265-1274 (2018). PubMed: 29286083
- Shi ZH et al. Mitochondrial ferritin, a new target for inhibiting neuronal tumor cell proliferation. Cell Mol Life Sci N/A:N/A (2014). WB . PubMed: 25213357