Overview

  • Product name

  • Description

    Rabbit polyclonal to JMJD6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Zebrafish
  • Immunogen

    A region within synthetic peptide: VLADSVDLQE STGIASDSSS DSSSSSSSSS SDSDSECESG SEGDGTVHRR, corresponding to C terminal amino acids 325-374 of Human JMJD6

  • Positive control

    • Fetal thymus lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab50720 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Predicted molecular weight: 46 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function

    Dioxygenase that can both act as a histone arginine demethylase and a lysyl-hydroxylase. Acts as a lysyl-hydroxylase that catalyzes 5-hydroxylation on specific lysine residues of target proteins such as U2AF2/U2AF65 and LUC7L2. Acts as a regulator of RNA splicing by mediating 5-hydroxylation of U2AF2/U2AF65, affecting the pre-mRNA splicing activity of U2AF2/U2AF65. In addition to peptidyl-lysine 5-dioxygenase activity, may act as a RNA hydroxylase, as suggested by its ability to bind single strand RNA. Also acts as an arginine demethylase which demethylates histone H3 at 'Arg-2' (H3R2me) and histone H4 at 'Arg-3' (H4R3me), thereby playing a role in histone code. However, histone arginine demethylation may not constitute the primary activity in vivo. Has no histone lysine demethylase activity. Required for differentiation of multiple organs during embryogenesis. Acts as a key regulator of hematopoietic differentiation: required for angiogenic sprouting by regulating the pre-mRNA splicing activity of U2AF2/U2AF65. Seems to be necessary for the regulation of macrophage cytokine responses.
  • Tissue specificity

    Highly expressed in the heart, skeletal muscle and kidney. Expressed at moderate or low level in brain, placenta, lung, liver, pancreas, spleen, thymus, prostate, testis and ovary. Up-regulated in many patients with chronic pancreatitis. Expressed in nursing thymic epithelial cells.
  • Sequence similarities

    Belongs to the JMJD6 family.
    Contains 1 JmjC domain.
  • Domain

    The nuclear localization signal motifs are necessary and sufficient to target it into the nucleus.
  • Cellular localization

    Nucleus > nucleoplasm. Nucleus > nucleolus. Mainly found throughout the nucleoplasm outside of regions containing heterochromatic DNA, with some localization in nucleolus. During mitosis, excluded from the nucleus and reappears in the telophase of the cell cycle.
  • Information by UniProt
  • Database links

  • Alternative names

    • Apoptotic cell clearance receptor antibody
    • Bifunctional arginine demethylase and lysyl-hydroxylase JMJD6 antibody
    • Histone arginine demethylase JMJD6 antibody
    • JmjC domain-containing protein 6 antibody
    • JMJD 6 antibody
    • JMJD6 antibody
    • JMJD6_HUMAN antibody
    • Jumonji domain containing 6 antibody
    • Jumonji domain-containing protein 6 antibody
    • KIAA0585 antibody
    • Lysyl-hydroxylase JMJD6 antibody
    • Peptide-lysine 5-dioxygenase JMJD6 antibody
    • Phosphatidylserine receptor antibody
    • Protein PTDSR antibody
    • PSR antibody
    • PTDSR 1 antibody
    • PTDSR antibody
    • PTDSR1 antibody
    see all

Images

  • Anti-JMJD6 antibody (ab50720) at 1.25 µg/ml + Fetal thymus lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 46 kDa



    Gel concentration 12%

References

This product has been referenced in:

  • Chung J  et al. Protein arginine methyltransferase 5 (PRMT5) promotes survival of lymphoma cells via activation of WNT/ß-catenin and AKT/GSK3ß proliferative signaling. J Biol Chem 294:7692-7710 (2019). Read more (PubMed: 30885941) »
  • Ruvolo PP  et al. Role of MSC-derived galectin 3 in the AML microenvironment. Biochim Biophys Acta 1865:959-969 (2018). Read more (PubMed: 29655803) »
See all 2 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Sample
Human Cell (293T)
Specification
293T
Permeabilization
Yes - Triton
Fixative
Formaldehyde

Herr Dr. Alexander Wolf

Verified customer

Submitted Jul 30 2014

Question
Answer

Thank you for contacting Abcam.

I am sorry this product has not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a blocking peptide (ab110802) to you with the order number 1021613.

To check the status of the order please contact our Customer Service team and reference this number.

I have also attached the protocol for use of immungen blocking peptides for reference.

Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Read More
Application
Western blot
Sample
Human Recombinant protein (N/A)
Loading amount
0.01 µg
Specification
N/A
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Feb 07 2008

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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