Anti-JMJD6 antibody (ab64575)
- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameAnti-JMJD6 antibody
See all JMJD6 primary antibodies -
DescriptionRabbit polyclonal to JMJD6
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Host speciesRabbit
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Tested applicationsSuitable for: ICC/IF, WBmore details
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Species reactivityReacts with: Mouse, Rat
Predicted to work with: Human
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human JMJD6.
(Peptide available as ab64574.)
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Positive control
- This antibody gave a positive signal in the following Tissue Lysates: Rat Liver, Mouse Liver, Mouse Heart
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
Applications
Our Abpromise guarantee covers the use of ab64575 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 1 µg/ml. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 46 kDa).Can be blocked with Human JMJD6 peptide (ab64574). |
Target
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FunctionDioxygenase that can both act as a histone arginine demethylase and a lysyl-hydroxylase. Acts as a lysyl-hydroxylase that catalyzes 5-hydroxylation on specific lysine residues of target proteins such as U2AF2/U2AF65 and LUC7L2. Acts as a regulator of RNA splicing by mediating 5-hydroxylation of U2AF2/U2AF65, affecting the pre-mRNA splicing activity of U2AF2/U2AF65. In addition to peptidyl-lysine 5-dioxygenase activity, may act as a RNA hydroxylase, as suggested by its ability to bind single strand RNA. Also acts as an arginine demethylase which demethylates histone H3 at 'Arg-2' (H3R2me) and histone H4 at 'Arg-3' (H4R3me), thereby playing a role in histone code. However, histone arginine demethylation may not constitute the primary activity in vivo. Has no histone lysine demethylase activity. Required for differentiation of multiple organs during embryogenesis. Acts as a key regulator of hematopoietic differentiation: required for angiogenic sprouting by regulating the pre-mRNA splicing activity of U2AF2/U2AF65. Seems to be necessary for the regulation of macrophage cytokine responses.
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Tissue specificityHighly expressed in the heart, skeletal muscle and kidney. Expressed at moderate or low level in brain, placenta, lung, liver, pancreas, spleen, thymus, prostate, testis and ovary. Up-regulated in many patients with chronic pancreatitis. Expressed in nursing thymic epithelial cells.
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Sequence similaritiesBelongs to the JMJD6 family.
Contains 1 JmjC domain. -
DomainThe nuclear localization signal motifs are necessary and sufficient to target it into the nucleus.
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Cellular localizationNucleus > nucleoplasm. Nucleus > nucleolus. Mainly found throughout the nucleoplasm outside of regions containing heterochromatic DNA, with some localization in nucleolus. During mitosis, excluded from the nucleus and reappears in the telophase of the cell cycle.
- Information by UniProt
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Database links
- Entrez Gene: 23210 Human
- Entrez Gene: 107817 Mouse
- Entrez Gene: 360665 Rat
- Omim: 604914 Human
- SwissProt: Q6NYC1 Human
- SwissProt: Q9ERI5 Mouse
- SwissProt: Q6AYK2 Rat
- Unigene: 514505 Human
see all -
Alternative names
- Apoptotic cell clearance receptor antibody
- Bifunctional arginine demethylase and lysyl-hydroxylase JMJD6 antibody
- Histone arginine demethylase JMJD6 antibody
see all
Images
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Western blot - Anti-JMJD6 antibody (ab64575)All lanes : Anti-JMJD6 antibody (ab64575) at 1 µg/ml
Lane 1 : Liver (Rat) Tissue Lysate
Lane 2 : Liver (Mouse) Tissue Lysate
Lane 3 : Heart (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 46 kDa
Observed band size: 50 kDa (why is the actual band size different from the predicted?)
Additional bands at: 26 kDa, 30 kDa, 43 kDa (possible isoform). We are unsure as to the identity of these extra bands.
Exposure time: 16 minutes -
Immunocytochemistry/ Immunofluorescence - Anti-JMJD6 antibody (ab64575)ICC/IF image of ab64575 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64575, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) Hek293, HepG2 and MCF7 cells at 1µg/ml, and in 4% formaldehyde fixed (10 min) Hek293, HepG2 and MCF7 cells at 1µg/ml.
References
This product has been referenced in:
- Miller TE et al. Transcription elongation factors represent in vivo cancer dependencies in glioblastoma. Nature 547:355-359 (2017). CHIPseq . Read more (PubMed: 28678782) »
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