Overview

  • Product name

  • Description

    Rabbit polyclonal to JMJD6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Zebrafish
  • Immunogen

    Recombinant protein fragment corresponding to a region within amino acids 1 and 244 of Human JMJD6 (NP_055982).

  • Positive control

    • 293T, A431, H1299, HeLa, HepG2, MOLT4, Cal27, Raji or NIH3T3 cell lines.

Properties

Applications

Our Abpromise guarantee covers the use of ab96795 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 46 kDa.
IHC-P 1/100 - 1/250.

Target

  • Function

    Dioxygenase that can both act as a histone arginine demethylase and a lysyl-hydroxylase. Acts as a lysyl-hydroxylase that catalyzes 5-hydroxylation on specific lysine residues of target proteins such as U2AF2/U2AF65 and LUC7L2. Acts as a regulator of RNA splicing by mediating 5-hydroxylation of U2AF2/U2AF65, affecting the pre-mRNA splicing activity of U2AF2/U2AF65. In addition to peptidyl-lysine 5-dioxygenase activity, may act as a RNA hydroxylase, as suggested by its ability to bind single strand RNA. Also acts as an arginine demethylase which demethylates histone H3 at 'Arg-2' (H3R2me) and histone H4 at 'Arg-3' (H4R3me), thereby playing a role in histone code. However, histone arginine demethylation may not constitute the primary activity in vivo. Has no histone lysine demethylase activity. Required for differentiation of multiple organs during embryogenesis. Acts as a key regulator of hematopoietic differentiation: required for angiogenic sprouting by regulating the pre-mRNA splicing activity of U2AF2/U2AF65. Seems to be necessary for the regulation of macrophage cytokine responses.
  • Tissue specificity

    Highly expressed in the heart, skeletal muscle and kidney. Expressed at moderate or low level in brain, placenta, lung, liver, pancreas, spleen, thymus, prostate, testis and ovary. Up-regulated in many patients with chronic pancreatitis. Expressed in nursing thymic epithelial cells.
  • Sequence similarities

    Belongs to the JMJD6 family.
    Contains 1 JmjC domain.
  • Domain

    The nuclear localization signal motifs are necessary and sufficient to target it into the nucleus.
  • Cellular localization

    Nucleus > nucleoplasm. Nucleus > nucleolus. Mainly found throughout the nucleoplasm outside of regions containing heterochromatic DNA, with some localization in nucleolus. During mitosis, excluded from the nucleus and reappears in the telophase of the cell cycle.
  • Information by UniProt
  • Database links

  • Alternative names

    • Apoptotic cell clearance receptor antibody
    • Bifunctional arginine demethylase and lysyl-hydroxylase JMJD6 antibody
    • Histone arginine demethylase JMJD6 antibody
    • JmjC domain-containing protein 6 antibody
    • JMJD 6 antibody
    • JMJD6 antibody
    • JMJD6_HUMAN antibody
    • Jumonji domain containing 6 antibody
    • Jumonji domain-containing protein 6 antibody
    • KIAA0585 antibody
    • Lysyl-hydroxylase JMJD6 antibody
    • Peptide-lysine 5-dioxygenase JMJD6 antibody
    • Phosphatidylserine receptor antibody
    • Protein PTDSR antibody
    • PSR antibody
    • PTDSR 1 antibody
    • PTDSR antibody
    • PTDSR1 antibody
    see all

Images

  • Anti-JMJD6 antibody (ab96795) at 1/1000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size: 46 kDa



    10% SDS PAGE
  • Immunohistochemistry of paraffin-embedded Human Cal27 xenograft using ab96795 at 1/100 dilution.

  • Anti-JMJD6 antibody (ab96795) at 1/1000 dilution + NIH 3T3 whole cell lysate at 30 µg

    Predicted band size: 46 kDa



    10% SDS PAGE

References

ab96795 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

We have found some reference data which indicates an apparent MW of 70 kD for JMJD6. Please see below: "The predicted 427-amino acid protein has 1 predicted transmembrane domain and an area in the C terminus that contains a series of serines that represent potential O-glycosylation sites. The predicted molecular weight for this protein based on sequence analysis was 47 to 48 kD for human, mouse, and Drosophila gene products and 40 kD for that of the nematode. Western blot analysis suggested an apparent molecular weight of 70 kD; deglycosylation reduces the apparent size to approximately 50 kD, which is compatible with the predicted molecular weight of 47 kD." From PubMed OMIM: http://www.ncbi.nlm.nih.gov/gene? Db=omim&DbFrom=gene&Cmd=Link&LinkName=gene_omim&LinkReadableName=OMIM&Id sFromResult=23210 I hope you find this information useful. Please let me know if I can provide further information.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up