Key features and details
- Rabbit polyclonal to JMY - C-terminal
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Chicken, Human
- Isotype: IgG
Product nameAnti-JMY antibody - C-terminal
See all JMY primary antibodies
DescriptionRabbit polyclonal to JMY - C-terminal
SpecificityThe immunogen has low homology to other WASP family members.
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Chicken, Human
Predicted to work with: Frog
Synthetic peptide coupled to a carrier protein, corresponding to C terminal amino acids of Mouse JMY.
- HeLa, Jurkat and PC12, Mouse brain and Mouse heart lysates; Chick fibroblasts.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 50% Glycerol, 0.1% BSA, 49% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab157586 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 111 kDa.|
FunctionActs both as a nuclear p53/TP53-cofactor and a cytoplasmic regulator of actin dynamics depending on conditions. In nucleus, acts as a cofactor that increases p53/TP53 response via its interaction with p300/EP300. Increases p53/TP53-dependent transcription and apoptosis, suggesting an important role in p53/TP53 stress response such as DNA damage. In cytoplasm, acts as a nucleation-promoting factor for both branched and unbranched actin filaments. Activates the Arp2/3 complex to induce branched actin filament networks. Also catalyzes actin polymerization in the absence of Arp2/3, creating unbranched filaments. Contributes to cell motility by controlling actin dynamics. May promote the rapid formation of a branched actin network by first nucleating new mother filaments and then activating Arp2/3 to branch off these filaments. The p53/TP53-cofactor and actin activator activities are regulated via its subcellular location.
Sequence similaritiesBelongs to the JMY family.
Contains 1 WH2 domain.
modificationsUbiquitinated by MDM2, leading to its subsequent degradation by the proteasome. In case of DNA damage, the interaction with MDM2 is altered, preventing degradation and allowing interaction with p300/EP300 and its function in p53/TP53 stress response.
Cellular localizationNucleus. Cytoplasm > cytoskeleton. Localizes to the nucleus in most cell types. Accumulates in nucleus under DNA damage conditions, increasing p53/TP53 transcription response and reducing its influence on cell motility (By similarity). In primary neutrophils, it colocalizes with actin filaments at the leading edge and is excluded from the nucleus. Localization correlates with motility, because it moves from the nucleus to the cytoplasmic compartment when cells are differentiated from nonmotile cells into highly motile neutrophil-like cells.
- Information by UniProt
- FLJ37870 antibody
- Jmy antibody
- JMY protein antibody
Immunofluorescent analysis of chick fibroblasts labeling JMY with ab157586 at 1/200 dilution (green); F-Actin (red).
All lanes : Anti-JMY antibody - C-terminal (ab157586) at 1/500 dilution
Lane 1 : Rat PC12 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : Adult Mouse heart lysate
Predicted band size: 111 kDa
ab157586 has not yet been referenced specifically in any publications.