Key features and details
- Rabbit polyclonal to JMY - C-terminal
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-JMY antibody - C-terminal
See all JMY primary antibodies
DescriptionRabbit polyclonal to JMY - C-terminal
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
- Human brain cortex tissue; COLO cell lysate
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 0.87% Sodium chloride, 50% Glycerol
PBS is without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab190212 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 10 µg/ml.|
|WB||1/500 - 1/1000. Predicted molecular weight: 111 kDa.|
FunctionActs both as a nuclear p53/TP53-cofactor and a cytoplasmic regulator of actin dynamics depending on conditions. In nucleus, acts as a cofactor that increases p53/TP53 response via its interaction with p300/EP300. Increases p53/TP53-dependent transcription and apoptosis, suggesting an important role in p53/TP53 stress response such as DNA damage. In cytoplasm, acts as a nucleation-promoting factor for both branched and unbranched actin filaments. Activates the Arp2/3 complex to induce branched actin filament networks. Also catalyzes actin polymerization in the absence of Arp2/3, creating unbranched filaments. Contributes to cell motility by controlling actin dynamics. May promote the rapid formation of a branched actin network by first nucleating new mother filaments and then activating Arp2/3 to branch off these filaments. The p53/TP53-cofactor and actin activator activities are regulated via its subcellular location.
Sequence similaritiesBelongs to the JMY family.
Contains 1 WH2 domain.
modificationsUbiquitinated by MDM2, leading to its subsequent degradation by the proteasome. In case of DNA damage, the interaction with MDM2 is altered, preventing degradation and allowing interaction with p300/EP300 and its function in p53/TP53 stress response.
Cellular localizationNucleus. Cytoplasm > cytoskeleton. Localizes to the nucleus in most cell types. Accumulates in nucleus under DNA damage conditions, increasing p53/TP53 transcription response and reducing its influence on cell motility (By similarity). In primary neutrophils, it colocalizes with actin filaments at the leading edge and is excluded from the nucleus. Localization correlates with motility, because it moves from the nucleus to the cytoplasmic compartment when cells are differentiated from nonmotile cells into highly motile neutrophil-like cells.
- Information by UniProt
- FLJ37870 antibody
- Jmy antibody
- JMY protein antibody
Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human brain, cortex, tissue labeling JMY using ab190212 at 10 µg/ml.
All lanes : Anti-JMY antibody - C-terminal (ab190212) at 1/500 dilution
Lane 1 : COLO cell lysate
Lane 2 : COLO cell lysate with Synthesized peptide
Predicted band size: 111 kDa
ab190212 has been referenced in 1 publication.
- Zuo W et al. Role of Junction-Mediating and Regulatory Protein in the Pathogenesis of Glucocorticoid-Induced Endothelial Cell Lesions. Orthop Surg 12:964-973 (2020). PubMed: 32363797