Recombinant
RabMAb

Recombinant Anti-JNK1 + JNK3 antibody [EPR16797-194] (ab191603)

Overview

  • Product name

    Anti-JNK1 + JNK3 antibody [EPR16797-194]
    See all JNK1 + JNK3 primary antibodies
  • Description

    Rabbit monoclonal [EPR16797-194] to JNK1 + JNK3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Cow, Dog, Human, Xenopus tropicalis
  • Immunogen

    Recombinant fragment within Human JNK1 + JNK3 aa 1-400. The exact sequence is proprietary. Also P53779 (92% homology).
    Database link: P45983

  • Positive control

    • WB: Full length human JNK1 and JNK3 recombinant proteins; K562, HeLa, Jurkat, C6, RAW 264.7, PC-12, NIH/3T3, Neuro-2a, MDCK and MDBK whole cell lysates; Mouse brain, mouse heart, mouse kidney and rat kidney lysates; Xenopus (X. tropicalis) muscle lysates. IP: K562 whole cell extract.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR16797-194
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab191603 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 54, 46 kDa (predicted molecular weight: 48 kDa).
IP 1/30.

Target

Images

  • All lanes : Anti-JNK1 + JNK3 antibody [EPR16797-194] (ab191603) at 1/2000 dilution

    Lane 1 : Full length human JNK1 recombinant protein
    Lane 2 : Full length human JNK2 recombinant protein
    Lane 3 : Full length human JNK3 recombinant protein

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 48 kDa
    Observed band size: 48,71 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Recombinant full length JNK1 protein contains aa1-427 with His-Tag®. Recombinant full length JNK2 protein contains aa1-424 with GST-tag and JNK3 protein contains aa1-464 with GST-tag.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-JNK1 + JNK3 antibody [EPR16797-194] (ab191603) at 1/2000 dilution

    Lane 1 : K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysate
    Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
    Lane 3 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 48 kDa
    Observed band size: 46,54 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-JNK1 + JNK3 antibody [EPR16797-194] (ab191603) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse heart lysate
    Lane 3 : Mouse kidney lysate
    Lane 4 : Rat kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 48 kDa
    Observed band size: 46,54 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-JNK1 + JNK3 antibody [EPR16797-194] (ab191603) at 1/1000 dilution

    Lane 1 : C6 (Rat glial tumor cells) whole cell lysate
    Lane 2 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
    Lane 4 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 48 kDa
    Observed band size: 46,54 kDa why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-JNK1 + JNK3 antibody [EPR16797-194] (ab191603) at 1/2000 dilution

    Lane 1 : Neuro-2a (Mouse neuroblastoma cells) whole cell lysate
    Lane 2 : Xenopus (X. tropicalis) muscle lysates
    Lane 3 : MDCK (Canine kidney cell line) whole cell lysate
    Lane 4 : MDBK (Bovine kidney cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 48 kDa
    Observed band size: 46,54 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • JNK1 + JNK3 were immunoprecipitated from 1mg of K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell extract with ab191603 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab191603 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: K562 whole cell extract 10 µg (Input).

    Lane 2: ab191603 IP in K562 whole cell extract.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab191603 in K562 whole cell extract.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

References

ab191603 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab191603.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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