Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal to JNK1 - BSA and Azide free (Capture)
- Suitable for: Sandwich ELISA
- Reacts with: Mouse
Product nameAnti-JNK1 antibody - BSA and Azide free (Capture)
See all JNK1 primary antibodies
DescriptionRabbit monoclonal to JNK1 - BSA and Azide free (Capture)
Tested applicationsSuitable for: Sandwich ELISAmore details
Species reactivityReacts with: Mouse
ab259519 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse JNK1. The recommended pair for sandwich ELISA is:
Capture: ab259519, Mouse JNK1 Capture Antibody (unconjugated)
Detector: ab259520, Mouse JNK1 Detector Antibody (unconjugated)
The reference range value is 391 - 25000 pg/ml.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is a recombinant monoclonal antibody, which offers several advantages including:
-High batch-to-batch consistency and reproducibility
-Improved sensitivity and specificity
-Long-term security of supply
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C.
Concentration information loading...
sELISA pair antibody
Our Abpromise guarantee covers the use of ab259519 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal to JNK1 - BSA and Azide free (Detector) (ab259520).|
FunctionResponds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.
Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain.
DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
modificationsDually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme.
- Information by UniProt
- AI849689 antibody
- c Jun N terminal kinase 1 antibody
- C-JUN kinase 1 antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab259519 has not yet been referenced specifically in any publications.