Validated using a knockout cell line

Anti-JNK1 antibody [EPR140(2)] (ab110724)


  • Product name
    Anti-JNK1 antibody [EPR140(2)]
    See all JNK1 primary antibodies
  • Description
    Rabbit monoclonal [EPR140(2)] to JNK1
  • Host species
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human JNK1 aa 300-400. The exact sequence is proprietary.

  • Positive control
    • HeLa, 293T, K562, and MCF7 cell lysates.
  • General notes

    A trial size is available to purchase for this antibody.


    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.


Associated products


Our Abpromise guarantee covers the use of ab110724 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 48 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Function
      Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
      JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.
    • Sequence similarities
      Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
      Contains 1 protein kinase domain.
    • Domain
      The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
    • Post-translational
      Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme.
    • Information by UniProt
    • Database links
    • Alternative names
      • AI849689 antibody
      • c Jun N terminal kinase 1 antibody
      • C-JUN kinase 1 antibody
      • c-Jun N-terminal kinase 1 antibody
      • EC antibody
      • JNK 1 antibody
      • JNK antibody
      • JNK-46 antibody
      • JNK1A2 antibody
      • JNK21B1/2 antibody
      • MAP kinase 8 antibody
      • MAPK 8 antibody
      • mapk8 antibody
      • Mitogen activated protein kinase 8 antibody
      • Mitogen-activated protein kinase 8 antibody
      • MK08_HUMAN antibody
      • p54 gamma antibody
      • Prkm8 antibody
      • Protein kinase JNK1 antibody
      • Protein kinase, mitogen-activated, 8 antibody
      • SAPK 1 antibody
      • SAPK gamma antibody
      • SAPK1 antibody
      • Stress-activated protein kinase 1 antibody
      • Stress-activated protein kinase JNK1 antibody
      see all


    • Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: JNK1 knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: MCF7 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab110724 observed at 46 and 54 kDa. Red - loading control, ab8226, observed at 42 kDa.

      ab110724 was shown to specifically react with JNK1 when JNK1 knockout samples were used. Wild-type and ProteinX knockout samples were subjected to SDS-PAGE. ab110724 and ab8226 (loading control to beta actin) were both diluted 1/1000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • All lanes : Anti-JNK1 antibody [EPR140(2)] (ab110724) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : K562 cell lysate
      Lane 4 : MCF7 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 48 kDa

    • Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: JNK1 knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: MCF7 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green).

      Green - target observed at 46 and 54 kDa. Red - loading control, ab8226, observed at 42 kDa.

      This western blot image is a comparison between ab110724 and a competitor's top cited mouse monoclonal antibody.



    This product has been referenced in:
    • Han X  et al. Mapping human pluripotent stem cell differentiation pathways using high throughput single-cell RNA-sequencing. Genome Biol 19:47 (2018). Read more (PubMed: 29622030) »
    • Wang N  et al. Hydroxytyrosol ameliorates insulin resistance by modulating endoplasmic reticulum stress and prevents hepatic steatosis in diet-induced obesity mice. J Nutr Biochem 57:180-188 (2018). Mouse . Read more (PubMed: 29747118) »

    See all 14 Publications for this product

    Customer reviews and Q&As

    Abcam has not validated the combination of species/application used in this Abreview.
    Western blot
    Cat Cell lysate - whole cell (synovial)
    Gel Running Conditions
    Reduced Denaturing (12%)
    Loading amount
    10 µg
    siRNA for scramble control, JNK1 and JNK2
    Blocking step
    (agent) for 50 minute(s) · Concentration: 100% · Temperature: 25°C

    令 中野

    Verified customer

    Submitted Jun 30 2017


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