Overview

  • Product name

    Anti-JNK1 antibody [EPR17557]
    See all JNK1 primary antibodies
  • Description

    Rabbit monoclonal [EPR17557] to JNK1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Chicken, Hamster, Dog, Human, Monkey, Xenopus tropicalis
  • Immunogen

    Recombinant fragment within Mouse JNK1 aa 200 to the C-terminus. The exact sequence is proprietary.
    Database link: Q91Y86

  • Positive control

    • WB: Full length human JNK1 recombinant protein; HeLa, U937, MCF7, Jurkat, C6, RAW 264.7, PC-12, NIH/3T3, MDCK, COS-1, UMNSAH/DF-1 and BHK whole cell lysates; Human fetal brain, fetal heart and fetal kidney lysates; Rat brain, heart and spleen lysates; Xenopus (X. tropicalis) muscle lysates.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab199380 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2500. Detects a band of approximately 54, 46 kDa (predicted molecular weight: 44 kDa).

Target

  • Function

    Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
    JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • Domain

    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • Post-translational
    modifications

    Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme.
  • Information by UniProt
  • Database links

  • Alternative names

    • AI849689 antibody
    • c Jun N terminal kinase 1 antibody
    • C-JUN kinase 1 antibody
    • c-Jun N-terminal kinase 1 antibody
    • EC 2.7.11.24 antibody
    • JNK 1 antibody
    • JNK antibody
    • JNK-46 antibody
    • JNK1A2 antibody
    • JNK21B1/2 antibody
    • MAP kinase 8 antibody
    • MAPK 8 antibody
    • mapk8 antibody
    • Mitogen activated protein kinase 8 antibody
    • Mitogen-activated protein kinase 8 antibody
    • MK08_HUMAN antibody
    • p54 gamma antibody
    • Prkm8 antibody
    • Protein kinase JNK1 antibody
    • Protein kinase, mitogen-activated, 8 antibody
    • SAPK 1 antibody
    • SAPK gamma antibody
    • SAPK1 antibody
    • Stress-activated protein kinase 1 antibody
    • Stress-activated protein kinase JNK1 antibody
    see all

Images

  • Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: JNK1 knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: MCF7 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab199380 observed at 46 and 54 kDa. Red - loading control, ab8226, observed at 42 kDa.

    ab199380 was shown to recognize JNK1 when JNK1 knockout samples were used, along with additional cross-reactive bands. Wild-type and JNK1 knockout samples were subjected to SDS-PAGE. ab199380 and ab8226 (loading control to beta Actin) were diluted to 1/2500 and 1/1000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

  • All lanes : Anti-JNK1 antibody [EPR17557] (ab199380) at 1/5000 dilution

    Lane 1 : Full length Human JNK1 recombinant protein
    Lane 2 : Full length Human JNK2 recombinant protein
    Lane 3 : Full length Human JNK3 recombinant protein

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 44 kDa
    Observed band size: 48 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Recombinant full length JNK1 protein contains aa1-427 with His-Tag®. Recombinant full length JNK2 protein contains aa1-424 with GST-tag and JNK3 protein contains aa1-464 with GST-tag.

  • All lanes : Anti-JNK1 antibody [EPR17557] (ab199380) at 1/10000 dilution

    Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
    Lane 2 : U937 (Human histiocytic lymphoma cells) whole cell lysate
    Lane 3 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
    Lane 4 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 44 kDa
    Observed band size: 46,54 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-JNK1 antibody [EPR17557] (ab199380) at 1/2500 dilution + Human fetal brain lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 44 kDa
    Observed band size: 46,54 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-JNK1 antibody [EPR17557] (ab199380) at 1/2500 dilution

    Lane 1 : Human fetal heart lysate
    Lane 2 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 44 kDa
    Observed band size: 46,54 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-JNK1 antibody [EPR17557] (ab199380) at 1/5000 dilution

    Lane 1 : Rat brain lysate
    Lane 2 : Rat heart lysate
    Lane 3 : Rat spleen lysate
    Lane 4 : C6 (Rat glial tumor cells) whole cell lysate
    Lane 5 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 6 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
    Lane 7 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 44 kDa
    Observed band size: 46,54 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-JNK1 antibody [EPR17557] (ab199380) at 1/2500 dilution

    Lane 1 : MDCK (Canine kidney cell line) whole cell lysates
    Lane 2 : COS-1 (African green monkey kidney fibroblast-like cell line) whole cell lysates
    Lane 3 : UMNSAH/DF-1 (Transformed chicken embyronic fibroblast cells) whole cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 44 kDa
    Observed band size: 46,54 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-JNK1 antibody [EPR17557] (ab199380) at 1/2500 dilution

    Lane 1 : Xenopus (X. tropicalis) muscle lysates
    Lane 2 : BHK (Hamster kidney fibroblast cells) whole cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 44 kDa
    Observed band size: 46,54 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

References

This product has been referenced in:

  • Ji J  et al. Kangxianling decoction prevents renal fibrosis in rats with 5/6 nephrectomy and inhibits Ang II-induced ECM production in glomerular mesangial cells. J Pharmacol Sci 139:367-372 (2019). Read more (PubMed: 30929858) »
  • Liang T  et al. Long noncoding RNA PEG10 facilitates bladder cancer cells proliferation, migration, and invasion via repressing microRNA-29b. J Cell Physiol N/A:N/A (2019). Read more (PubMed: 30941768) »
See all 9 Publications for this product

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