Overview

  • Product name

    Anti-JNK1 antibody [EPR18841-99]
    See all JNK1 primary antibodies
  • Description

    Rabbit monoclonal [EPR18841-99] to JNK1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human JNK1 aa 150-400. The exact sequence is proprietary.
    Database link: P45983

  • Positive control

    • WB: Human JNK1 full length recombinant protein; Human fetal brain, fetal heart and fetal kidney lysates; Jurkat, HeLa, K562, MCF7, C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; Mouse brain, kidney and spleen lysates; Rat brain, heart, kidney and spleen lysates. IP: HeLa whole cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab213521 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 46-54 kDa (predicted molecular weight: 48 kDa).
IP 1/40.

Target

  • Function

    Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
    JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • Domain

    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • Post-translational
    modifications

    Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme.
  • Information by UniProt
  • Database links

  • Alternative names

    • AI849689 antibody
    • c Jun N terminal kinase 1 antibody
    • C-JUN kinase 1 antibody
    • c-Jun N-terminal kinase 1 antibody
    • EC 2.7.11.24 antibody
    • JNK 1 antibody
    • JNK antibody
    • JNK-46 antibody
    • JNK1A2 antibody
    • JNK21B1/2 antibody
    • MAP kinase 8 antibody
    • MAPK 8 antibody
    • mapk8 antibody
    • Mitogen activated protein kinase 8 antibody
    • Mitogen-activated protein kinase 8 antibody
    • MK08_HUMAN antibody
    • p54 gamma antibody
    • Prkm8 antibody
    • Protein kinase JNK1 antibody
    • Protein kinase, mitogen-activated, 8 antibody
    • SAPK 1 antibody
    • SAPK gamma antibody
    • SAPK1 antibody
    • Stress-activated protein kinase 1 antibody
    • Stress-activated protein kinase JNK1 antibody
    see all

Images

  • All lanes : Anti-JNK1 antibody [EPR18841-99] (ab213521) at 1/1000 dilution

    Lane 1 : Human JNK1 full length recombinant protein
    Lane 2 : Human JNK2 full length recombinant protein
    Lane 3 : Human JNK3 full length recombinant protein

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 48 kDa
    Observed band size: 48 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 1 second; Lane 2 and 3: 3 minutes.

    Human JNK1 full length recombinant protein contain aa1-427 with a His-Tag®. Human JNK2 full length recombinant protein contain aa1-424 with a His-Tag®. Human JNK3 full length recombinant protein contain aa1-464 with a His-Tag®.

  • All lanes : Anti-JNK1 antibody [EPR18841-99] (ab213521) at 1/1000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Human fetal heart lysate
    Lane 3 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 48 kDa
    Observed band size: 46-54 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 11313405).

  • All lanes : Anti-JNK1 antibody [EPR18841-99] (ab213521) at 1/1000 dilution

    Lane 1 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 3 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
    Lane 4 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 48 kDa
    Observed band size: 46-54 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    observed is consistent with what has been described in the literature (PMID: 11313405).

  • All lanes : Anti-JNK1 antibody [EPR18841-99] (ab213521) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse kidney lysate
    Lane 3 : Mouse spleen lysate
    Lane 4 : Rat brain lysate
    Lane 5 : Rat heart lysate
    Lane 6 : Rat kidney lysate
    Lane 7 : Rat spleen lysate
    Lane 8 : C6 (Rat glial tumor cell line) whole cell lysate
    Lane 9 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 10 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 11 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 48 kDa
    Observed band size: 46-54 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1,2,3,4,5,6 and 7: 3 minutes; Lane 8,9,10 and 11: 15 seconds.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 11313405).

  • All lanes : Anti-JNK1 antibody [EPR18841-99] (ab213521) at 1/1000 dilution

    Lane 1 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 2 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate with Human JNK1 recombinant protein fragment (immunogen).

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 48 kDa
    Observed band size: 46-54 kDa why is the actual band size different from the predicted?


    Exposure time: 3 seconds
  • JNK1 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab213521 at 1/40 dilution.

    Western blot was performed from the immunoprecipitate using ab213521 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

    Lane 1: HeLa whole cell lysate, 10ug (Input).

    Lane 2: ab213521 IP in HeLa whole cell lysate.

    Lane 3: Rabbit IgG,monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab213521 in HeLa whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

References

This product has been referenced in:

  • Liang X  et al. Reactive oxygen species mediated oxidative stress links diabetes and atrial fibrillation. Mol Med Rep 17:4933-4940 (2018). Read more (PubMed: 29393403) »
  • Deng G  et al. BMP4 promotes hepatocellular carcinoma proliferation by autophagy activation through JNK1-mediated Bcl-2 phosphorylation. J Exp Clin Cancer Res 37:156 (2018). Read more (PubMed: 30012194) »
See all 2 Publications for this product

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