Recombinant
RabMAb

Recombinant Anti-JNK1 (phospho T183 + Y185) antibody [EPR20763] (ab215208)

Overview

  • Product name

    Anti-JNK1 (phospho T183 + Y185) antibody [EPR20763]
    See all JNK1 primary antibodies
  • Description

    Rabbit monoclonal [EPR20763] to JNK1 (phospho T183 + Y185)
  • Host species

    Rabbit
  • Specificity

    Based on sequence homology this antibody cross reacts with JNK2 (phospho T183/Y185) and JNK3 (phospho T221/Y223).
  • Tested applications

    Suitable for: Dot blot, IP, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human JNK1 aa 150-250. The exact sequence is proprietary.
    Database link: P45983

  • Positive control

    • WB: HEK-293 treated with 40 J/m² UV-C light, then cultivated in RPMI 1640 media containing 10% FBS for 30 minutes and U-2 OS treated with 50 J/m² UV-C light, then cultivated in RPMI 1640 media containing 10% FBS for 2 hours cell lysate. Dot Blot: JNK1 (phospho T183/Y185) peptide. IP: HEK-293 treated with 40 J/m² UV-C light, then cultivated in RPMI 1640 media containing 10% FBS for 30 minutes cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab215208 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot 1/1000.
IP 1/30.
WB 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).

Target

  • Function

    Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
    JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • Domain

    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • Post-translational
    modifications

    Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme.
  • Information by UniProt
  • Database links

  • Alternative names

    • AI849689 antibody
    • c Jun N terminal kinase 1 antibody
    • C-JUN kinase 1 antibody
    • c-Jun N-terminal kinase 1 antibody
    • EC 2.7.11.24 antibody
    • JNK 1 antibody
    • JNK antibody
    • JNK-46 antibody
    • JNK1A2 antibody
    • JNK21B1/2 antibody
    • MAP kinase 8 antibody
    • MAPK 8 antibody
    • mapk8 antibody
    • Mitogen activated protein kinase 8 antibody
    • Mitogen-activated protein kinase 8 antibody
    • MK08_HUMAN antibody
    • p54 gamma antibody
    • Prkm8 antibody
    • Protein kinase JNK1 antibody
    • Protein kinase, mitogen-activated, 8 antibody
    • SAPK 1 antibody
    • SAPK gamma antibody
    • SAPK1 antibody
    • Stress-activated protein kinase 1 antibody
    • Stress-activated protein kinase JNK1 antibody
    see all

Images

  • All lanes : Anti-JNK1 (phospho T183 + Y185) antibody [EPR20763] (ab215208) at 1/1000 dilution

    Lane 1 : Untreated HEK-293 (human epithelial cell line from embryonic kidney) cultivated in RPMI 1640 media containing 10% FBS for 30 minutes, whole cell lysate
    Lane 2 : HEK-293 treated with 40 J/m² UV-C light, then cultivated in RPMI 1640 media containing 10% FBS for 30 minutes, whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 48 kDa
    Observed band size: 46, 54 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 minute


    Blocking/Dilution: 5% NFDM/TBST.

    Phosphorylation of JNK at T183/Y185 can be induced by UV-C treatment (PMID: 15386344).

  • Dot blot analysis of JNK1 (phospho T183+ Y185) labeled with ab215208 at 1/1000 dilution.

    Lane 1: JNK1 (phospho T183/Y185) peptide.
    Lane 2: JNK1 (phospho T183) peptide.
    Lane 3: JNK1 (phospho Y185) peptide.
    Lane 4: JNK1 non-phospho peptide.

    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100,000 dilution was used as secondary antibody.

    Based on sequence homology this antibody also reacts with JNK2 (phospho T183/Y185) and JNK3 (phospho T221/Y223).

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • JNK1 (phospho T183 + Y185) was immunoprecipitated from 0.35mg of HEK-293 (human epithelial cell line from embryonic kidney) treated with 40 J/m² UV-C light, then cultivated in RPMI 1640 media containing 10% FBS for 30 minutes whole cell lysate with ab215208 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab215208 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

    Lane 1: HEK-293 (human epithelial cell line from embryonic kidney) treated with 40 J/m² UV-C light, then cultivated in RPMI 1640 media containing 10% FBS for 30 minutes whole cell lysate 10 µg (Input). 

    Lane 2: ab215208 IP in HEK-293 treated with 40 J/m² UV-C light, then cultivated in RPMI 1640 media containing 10% FBS for 30 minutes whole cell lysate (+). 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab215208 in HEK-293 treated with 40 J/m² UV-C light, then cultivated in RPMI 1640 media containing 10% FBS for 30 minutes whole cell lysate (-).

    Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 3 minutes.

  • All lanes : Anti-JNK1 (phospho T183 + Y185) antibody [EPR20763] (ab215208) at 1/1000 dilution

    Lane 1 : Untreated U-2 OS (human bone osteosarcoma epithelial cell line) cultivated in RPMI 1640 media containing 10% FBS for 2 hours, whole cell lysate
    Lane 2 : U-2 OS treated with 50 J/m² UV-C light, then cultivated in RPMI 1640 media containing 10% FBS for 2 hours, whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 48 kDa
    Observed band size: 46, 54 kDa why is the actual band size different from the predicted?



    Blocking/Dilution: 5% NFDM/TBST.

    Phosphorylation of JNK at T183/Y185 can be induced by UV-C treatment (PMID: 15386344).

References

ab215208 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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