• Product name
  • Description
    Rabbit polyclonal to JNK1+JNK2
  • Host species
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 300 - 400 of Human JNK1+JNK2.

    (Peptide available as ab95423.)

  • Positive control
    • This antibody gave a positive signal in human brain tissue lysate, and the following whole cell lysates: HEK293; HepG2; Jurkat; HeLa; Irradiated HeLa.



Our Abpromise guarantee covers the use of ab85139 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa). Also detects a band of approximately 44-kDa.


  • Function
    Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
    JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • Domain
    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • Post-translational
    Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme.
  • Information by UniProt
  • Database links
  • Alternative names
    • c jun N terminal kinase 2 antibody
    • c-Jun N-terminal kinase 1 antibody
    • cJun N terminal kinase 1 antibody
    • JNK 1 antibody
    • JNK 2 antibody
    • JNK-46 antibody
    • JNK2ALPHA antibody
    • JNK2BETA antibody
    • MAP kinase 8 antibody
    • MAP kinase 9 antibody
    • MAPK 8 antibody
    • mapk8 antibody
    • MAPK9 antibody
    • Mitogen activated protein kinase 8 antibody
    • Mitogen activated protein kinase 9 antibody
    • Mitogen-activated protein kinase 8 antibody
    • MK08_HUMAN antibody
    • PRKM 8 antibody
    • PRKM 9 antibody
    • Stress-activated protein kinase 1 antibody
    • Stress-activated protein kinase JNK1 antibody
    see all


  • All lanes : Anti-JNK1+JNK2 antibody (ab85139) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 6 : Irradiated HeLa

    Lysates/proteins at 10 µg per lane.

    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 48 kDa
    Observed band size: 48 kDa
    Additional bands at: 44 kDa (possible isoform), 54 kDa (possible post-translational modification)

    Exposure time: 3 minutes

    JNK1 has 4 isoforms.  JNK1 alpha 2 and JNK1 beta 2 are both 48-kDa.  JNK alpha 1 and JNK1 beta 1 are 44-kDa.  ab85139 detects bands at approximately 44-kDa, 48-kDa and 54-kDa.  We hypothesise that the bands at 44-kDa and 48-kDa correspond to different isoforms of JNK1.  JNK1 has several potential phosphorylation sites (Swissprot).  The band at 54-kDa may be a phosphorylated form of the JNK1 protein.   


This product has been referenced in:
  • Guo CH  et al. The analgesic effect of rolipram is associated with the inhibition of the activation of the spinal astrocytic JNK/CCL2 pathway in bone cancer pain. Int J Mol Med 38:1433-1442 (2016). WB . Read more (PubMed: 28025994) »
  • Krishna SN  et al. A fluorescence-based thermal shift assay identifies inhibitors of mitogen activated protein kinase kinase 4. PLoS One 8:e81504 (2013). WB . Read more (PubMed: 24339940) »

See all 2 Publications for this product

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